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AB193696

Human CKMB ELISA Kit

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(4 Publications)

Human CKMB ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CKMB in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CKMM, CKM, Creatine kinase M-type, Creatine kinase M chain, Creatine phosphokinase M-type, M-CK, CPK-M

1 Images
Sandwich ELISA - Human CKMB ELISA Kit (AB193696)
  • sELISA

Supplier Data

Sandwich ELISA - Human CKMB ELISA Kit (AB193696)

Example Standard Curve

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.3 ng/mL

Range

0.3 - 80 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CKMB Human ELISA Kit (ab193696) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CKMB in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human CKMB coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures CKMB present in the samples. The wells are washed and biotinylated anti-Human CKMB antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of CKMB bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "71 - 123 %", "average": "= 97.17" }, { "sample": "Plasma", "range": "78 - 108 %", "average": "= 87.57" }, { "sample": "Cell culture media", "range": "67 - 106 %", "average": "= 86.45" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193696-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193696-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193696-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CKMB Standards (lyophilized)", "number":"AB193696-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated Human CKMB Microplate (12 strips x 8 wells)", "number":"AB193696-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193696-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193696-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human CKMB detection antibody", "number":"AB193696-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin Concentrate", "number":"AB193696-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB193696-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
See full target information CKM
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Environmental pollution (Barking, Essex : 1987) 355:124151 PubMed38740242

2024

Elevated plasma solMER concentrations link ambient PM and PAHs to myocardial injury and reduced left ventricular systolic function in children.

Applications

Unspecified application

Species

Unspecified reactive species

Zihan Chen,Xia Huo,Yu Huang,Zhiheng Cheng,Xijin Xu,Zhi Li

Nature protocols 16:2564-2593 PubMed33911259

2021

Microfluidic integration of regeneratable electrochemical affinity-based biosensors for continual monitoring of organ-on-a-chip devices.

Applications

Unspecified application

Species

Unspecified reactive species

Julio Aleman,Tugba Kilic,Luis S Mille,Su Ryon Shin,Yu Shrike Zhang

Molecular therapy. Nucleic acids 23:1304-1322 PubMed33717651

2021

Exosomal LINC00174 derived from vascular endothelial cells attenuates myocardial I/R injury via p53-mediated autophagy and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Su,Xiang-Wei Lv,Yu-Li Xu,Ru-Ping Cai,Ri-Xin Dai,Xi-Heng Yang,Wei-Kun Zhao,Bing-Hui Kong

Frontiers in pediatrics 6:139 PubMed29977885

2018

Improves Peripheral Treg Cell Immunity Imbalance in the Children With Viral Myocarditis by Reducing the Levels of miR-146b and miR-155.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Zhang,Xinlun Dai,Ji Qi,Yu Ao,Chunfeng Yang,Yumei Li
View all publications
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