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Human CKMB ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CKMB in Human in Plasma, Cell culture media, Serum samples.

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Sandwich ELISA - Human CKMB ELISA Kit (AB193696), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture media, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.3 - 80 ng/mL
Sensitivity
= 0.3 ng/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Alternative names

CKMM, CKM, Creatine kinase M-type, Creatine kinase M chain, Creatine phosphokinase M-type, M-CK, CPK-M

What's included?

1 x 96 Tests
Components
100X HRP-Streptavidin Concentrate
1 x 200 µL
20X Wash Buffer
1 x 25 mL
5X Assay Diluent B
1 x 15 mL
5X Assay Diluent D
1 x 15 mL
Biotinylated Human CKMB detection antibody
2 x 1 Vial
Human CKMB Standards (lyophilized)
2 x 1 Vial
Pre-coated Human CKMB Microplate (12 strips x 8 wells)
1 x 1 Unit
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human CKMB ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CKMB in Human in Plasma, Cell culture media, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture media, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.3 - 80 ng/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 0.3 ng/mL

Precision

Intra assay

Sample
Overall
n
0
mean
-
SD
-
C.V.
< 10

Inter assay

Sample
Overall
n
0
mean
-
SD
-
C.V.
< 12

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 97.17
Range
71 - 123 %
Sample type
Plasma
Average %
= 87.57
Range
78 - 108 %
Sample type
Cell culture media
Average %
= 86.45
Range
67 - 106 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Abcam's CKMB Human ELISA Kit (ab193696) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CKMB in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human CKMB coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures CKMB present in the samples. The wells are washed and biotinylated anti-Human CKMB antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of CKMB bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human CKMB ELISA Kit (ab193696), expandable thumbnail

    Sandwich ELISA - Human CKMB ELISA Kit (ab193696)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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