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AB277413

Human CLEC4C ELISA Kit

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Human CLEC4C ELISA Kit is a Sandwich ELISA for the measurement of Human CLEC4C in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD303, BDCA2, CLECSF11, CLECSF7, DLEC, HECL, UNQ9361/PRO34150, CLEC4C, C-type lectin domain family 4 member C, Blood dendritic cell antigen 2, C-type lectin superfamily member 7, Dendritic lectin, BDCA-2

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ELISA - Human CLEC4C ELISA Kit (AB277413)
  • ELISA

Supplier Data

ELISA - Human CLEC4C ELISA Kit (AB277413)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.082 ng/mL

Range

0.082 - 20 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CLEC4C ELISA Kit (ab277413) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CLEC4C in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human CLEC4C coated on a 96-well plate. Standards and samples are pipetted into the wells and Human CLEC4C present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human CLEC4C antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human CLEC4C bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "72 - 97 %", "average": "= 87.73" }, { "sample": "Plasma", "range": "75 - 87 %", "average": "= 82.11" }, { "sample": "Cell culture media", "range": "71 - 135 %", "average": "= 105.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277413-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277413-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277413-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277413-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human CLEC4C coated Microplate", "number":"AB277413-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CLEC4C standard protein (Lyophilized)", "number":"AB277413-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB277413-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human CLEC2C Antibody", "number":"AB277413-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277413-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Lectin-type cell surface receptor which may play a role in antigen capturing by dendritic cells (PubMed : 11748283, PubMed : 21880719, PubMed : 25995448). Specifically recognizes non-sialylated galactose-terminated biantennary glycans containing the trisaccharide epitope Gal(beta1-3/4)GlcNAc(beta1-2)Man (PubMed : 21880719, PubMed : 25995448). Binds to serum IgG (PubMed : 25995448). Efficiently targets ligand into antigen-processing and peptide-loading compartments for presentation to T-cells (PubMed : 11748283). May mediate potent inhibition of induction of IFN-alpha/beta expression in plasmacytoid dendritic cells (PubMed : 11748283, PubMed : 21880719). May act as a signaling receptor that activates protein-tyrosine kinases and mobilizes intracellular calcium (PubMed : 11748283).
See full target information CLEC4C
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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