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AB108771

Human Anti-Clostridium tetani toxin IgG ELISA Kit

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(1 Publication)

Human Anti-Clostridium tetani toxin IgG ELISA Kit is an indirect ELISA for the qualitative detection of IgG class antibodies against Clostridium tetani toxin in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included
1 Images
Indirect ELISA - Human Anti-Clostridium tetani toxin IgG ELISA Kit (AB108771)
  • I-ELISA

Supplier Data

Indirect ELISA - Human Anti-Clostridium tetani toxin IgG ELISA Kit (AB108771)

Representative Standard Curve using ab108771

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Sensitivity

< 0.01 IU/mL

Range

0.01 - 1 IU/mL

Assay Platform

Microplate

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Reactivity data

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Product details

Abcam's anti-Clostridium tetani toxin IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Clostridium tetani toxin in Human serum and plasma.

A 96-well plate has been precoated with Clostridium tetani toxin antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Clostridium tetani toxin-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Clostridium tetani toxin IgG sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 24, "mean": "1.24 U/mL", "standardDeviation": null, "coefficientOfVariability": "= 4.9" }, { "reproducibilityType": "Intra", "sample": "Weak + Serum", "replicates": 24, "mean": "0.56 U/mL", "standardDeviation": null, "coefficientOfVariability": "= 7.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 10, "mean": "0.54 U/mL", "standardDeviation": null, "coefficientOfVariability": "= 7.7" }, { "reproducibilityType": "Inter", "sample": "Weak + Serum", "replicates": 10, "mean": "0.22 U/mL", "standardDeviation": null, "coefficientOfVariability": "= 11.3" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Clostridium tetani toxin IgG also known as tetanus toxoid antibody targets the tetanospasmin produced by the bacterium Clostridium tetani. Tetanus toxin is a notable neurotoxic protein with a molecular mass of approximately 150 kDa. The toxin is expressed primarily by Clostridium tetani bacteria in contaminated wounds or environments such as soil or animal excretion. This protein targets neurons by inhibiting neurotransmitter release which can result in motor function impairment.
Biological function summary

The role of Clostridium tetani toxin involves its activity as a potent neurotoxin causing violent muscular contractions. The toxin enters motor neurons and travels retroaxonally to the central nervous system preventing the release of inhibitory neurotransmitters like glycine and gamma-aminobutyric acid (GABA). It does not form part of a complex but independently exerts its significant effects on the nervous system.

Pathways

Tetanus toxin adversely affects normal neurotransmission processes. It specifically interrupts synaptic vesicle release pathways by cleaving synaptobrevin a critical protein for neurotransmitter vesicle fusion. This action interferes with the Wnt signaling pathway and affects proteins related to synaptic vesicle recycling including synaptotagmin and SNAP-25 further exacerbating the impairment of neurotransmission.

Clostridium tetani toxin is directly involved in tetanus a life-threatening condition characterized by muscle stiffness and spasms. The disease can lead to severe complications such as respiratory failure if untreated. Additionally the toxin's disruption of inhibitory neurotransmitters links it to motor disorders wherein imbalance in neural signaling plays critical roles. Tetanospasmin has a significant connection with neural proteins like synaptobrevin influencing symptoms associated with nerve and muscle disorders.
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 106:105249 PubMed39024898

2024

Sustained chronic inflammation and altered childhood vaccine responses in children exposed to Zika virus.

Applications

Unspecified application

Species

Unspecified reactive species

Suan-Sin Foo,Weiqiang Chen,Tamiris Azamor,Kyle L Jung,Mary Catherine Cambou,Débora Familiar-Macedo,Gielenny M Salem,Ivonne Melano,Myung-Shin Sim,Maria Elisabeth Moreira,Patricia Brasil,Zilton Vasconcelos,Karin Nielsen-Saines,Jae U Jung
View all publications
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websiteProtocolBooklet
en
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Product promise

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