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Human Anti-Clostridium tetani toxin IgG ELISA Kit is a Indirect ELISA kit for the measurement of Human Anti-Clostridium tetani toxin IgG in Human in Citrate plasma, Heparin Plasma, Serum samples.
- Detection method
Colorimetric
- Sample types
Citrate plasma, Heparin Plasma, Serum
- Assay type
Indirect
- Reactive species
Human
- Range
0.01 - 1 IU/mL
- Sensitivity
< 0.01 IU/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
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Human Anti-Clostridium tetani toxin IgG ELISA Kit is a Indirect ELISA kit for the measurement of Human Anti-Clostridium tetani toxin IgG in Human in Citrate plasma, Heparin Plasma, Serum samples.
Key facts
- Detection method
Colorimetric
- Sample types
Citrate plasma, Heparin Plasma, Serum
- Assay type
Indirect
- Reactive species
Human
- Range
0.01 - 1 IU/mL
- Assay Platform
Microplate
- Sensitivity
< 0.01 IU/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 24 | mean 1.24 U/mL | SD - | C.V. = 4.9 |
Sample Weak + Serum | n 24 | mean 0.56 U/mL | SD - | C.V. = 7.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 10 | mean 0.54 U/mL | SD - | C.V. = 7.7 |
Sample Weak + Serum | n 10 | mean 0.22 U/mL | SD - | C.V. = 11.3 |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Abcam's anti-Clostridium tetani toxin IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Clostridium tetani toxin in Human serum and plasma.
A 96-well plate has been precoated with Clostridium tetani toxin antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Clostridium tetani toxin-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Clostridium tetani toxin IgG sample captured in plate.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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