Human CLSTN1 ELISA Kit- extracellular is a single-wash 90-min Simplestep used to quantify Human CLSTN1 with a sensitivity of 48.36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Postsynaptic adhesion molecule that binds to presynaptic neurexins to mediate both excitatory and inhibitory synapse formation (By similarity). Promotes synapse development by acting as a cell adhesion molecule at the postsynaptic membrane, which associates with neurexin-alpha at the presynaptic membrane (By similarity). Also functions as a cargo in axonal anterograde transport by acting as a molecular adapter that promotes KLC1 association with vesicles (PubMed:21385839). Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation (PubMed:12972431). Soluble Alc-alpha. As intracellular fragment AlcICD, suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding (PubMed:15037614). CTF1-alpha. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP (PubMed:15037614).
CS1, KIAA0911, CLSTN1, Calsyntenin-1, Alcadein-alpha, Alzheimer-related cadherin-like protein, Non-classical cadherin XB31alpha, Alc-alpha
Human CLSTN1 ELISA Kit- extracellular is a single-wash 90-min Simplestep used to quantify Human CLSTN1 with a sensitivity of 48.36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 9.5 |
Sample Serum | n 8 | mean - | SD - | C.V. 2.5 |
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 2.5 |
Sample Serum | n 3 | mean - | SD - | C.V. 4 |
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 111 | Range 97 - 125 % |
Sample type EDTA Plasma | Average % = 101 | Range 97 - 105 % |
Sample type Citrate plasma | Average % = 92 | Range 85 - 105 % |
Sample type Heparin Plasma | Average % = 91 | Range 66 - 108 % |
Sample type Cerebral Spinal Fluid | Average % = 112 | Range 109 - 115 % |
Sample type Cell culture supernatant | Average % = 99 | Range 89 - 106 % |
Human CLSTN1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CLSTN1 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Cerebral Spinal Fluid, Cell culture supernatant. Quantitate Human CLSTN1 with 48.36 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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