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AB316262

Human CLSTN1 ELISA Kit- extracellular

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Human CLSTN1 ELISA Kit- extracellular is a single-wash 90-min Simplestep used to quantify Human CLSTN1 with a sensitivity of 48.36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CS1, KIAA0911, CLSTN1, Calsyntenin-1, Alcadein-alpha, Alzheimer-related cadherin-like protein, Non-classical cadherin XB31alpha, Alc-alpha

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Sandwich ELISA - Human CLSTN1 ELISA Kit- extracellular (AB316262)
  • sELISA

Supplier Data

Sandwich ELISA - Human CLSTN1 ELISA Kit- extracellular (AB316262)

Example of human CLSTN1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CLSTN1 ELISA Kit- extracellular (AB316262)
  • sELISA

Supplier Data

Sandwich ELISA - Human CLSTN1 ELISA Kit- extracellular (AB316262)

Interpolated concentration of native CLSTN1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 12.5, plasma (citrate) 1 : 12.5, plasma (EDTA) 1 : 12.5, plasma (heparin) 1 : 12.5, and U-87 MG cell culture supernatant 15%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human CLSTN1 ELISA Kit- extracellular (AB316262)
  • sELISA

Supplier Data

Sandwich ELISA - Human CLSTN1 ELISA Kit- extracellular (AB316262)

Example of human CLSTN1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CLSTN1 ELISA Kit- extracellular (AB316262)
  • sELISA

Supplier Data

Sandwich ELISA - Human CLSTN1 ELISA Kit- extracellular (AB316262)

Interpolated concentration of native CLSTN1 was measured in duplicate at different sample concentrations. The undiluted sample is 1 : 67 CSF. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 10BS.

Sandwich ELISA - Human CLSTN1 ELISA Kit- extracellular (AB316262)
  • sELISA

Supplier Data

Sandwich ELISA - Human CLSTN1 ELISA Kit- extracellular (AB316262)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CLSTN1 concentration was determined to be 30,644 pg/mL with a range of 20,727 – 40,091 pg/mL.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Cerebral Spinal Fluid, Cell culture supernatant, Citrate plasma, Heparin Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 48.36 pg/mL

Range

54.688 - 3500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CLSTN1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CLSTN1 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Cerebral Spinal Fluid, Cell culture supernatant. Quantitate Human CLSTN1 with 48.36 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]
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Recovery

[ { "sample": "Serum", "range": "97 - 125 %", "average": "= 111" }, { "sample": "EDTA Plasma", "range": "97 - 105 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "85 - 105 %", "average": "= 92" }, { "sample": "Heparin Plasma", "range": "66 - 108 %", "average": "= 91" }, { "sample": "Cerebral Spinal Fluid", "range": "109 - 115 %", "average": "= 112" }, { "sample": "Cell culture supernatant", "range": "89 - 106 %", "average": "= 99" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab316262-96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB316262-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB316262-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CLSTN1 Lyophilized Recombinant Protein", "number":"AB316262-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CLSTN1 Capture Antibody", "number":"AB316262-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB316262-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB316262-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB316262-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BS", "number":"AB316262-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB316262-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB316262-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CLSTN1 Detector Antibody", "number":"AB316262-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Postsynaptic adhesion molecule that binds to presynaptic neurexins to mediate both excitatory and inhibitory synapse formation (By similarity). Promotes synapse development by acting as a cell adhesion molecule at the postsynaptic membrane, which associates with neurexin-alpha at the presynaptic membrane (By similarity). Also functions as a cargo in axonal anterograde transport by acting as a molecular adapter that promotes KLC1 association with vesicles (PubMed : 21385839). Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation (PubMed : 12972431).. Soluble Alc-alpha. As intracellular fragment AlcICD, suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding (PubMed : 15037614).. CTF1-alpha. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP (PubMed : 15037614).
See full target information CLSTN1
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB134130

Anti-CLSTN1 antibody [EPR2963]

RabMAb|Recombinant

Western blot - Anti-CLSTN1 antibody [EPR2963] (AB134130)

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