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AB316260

Human CLSTN1 ELISA Kit- intracellular

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Human CLSTN1 ELISA Kit- intracellular is a single-wash 90-min Simplestep used to quantify Human CLSTN1 with a sensitivity of 11 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CS1, KIAA0911, CLSTN1, Calsyntenin-1, Alcadein-alpha, Alzheimer-related cadherin-like protein, Non-classical cadherin XB31alpha, Alc-alpha

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Sandwich ELISA - Human CLSTN1 ELISA Kit- intracellular (AB316260)
  • sELISA

Supplier Data

Sandwich ELISA - Human CLSTN1 ELISA Kit- intracellular (AB316260)

Interpolated concentration of native CLSTN1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : U-87 MG extract 700 µg/mL, SH-SY5Y extract 1,200 µg/mL, and brain cortex extract 90 µg/mL.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human CLSTN1 ELISA Kit- intracellular (AB316260)
  • sELISA

Supplier Data

Sandwich ELISA - Human CLSTN1 ELISA Kit- intracellular (AB316260)

Example of human CLSTN1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CLSTN1 ELISA Kit- intracellular (AB316260)
  • sELISA

Supplier Data

Sandwich ELISA - Human CLSTN1 ELISA Kit- intracellular (AB316260)

Interpolated concentration of native CLSTN1 was measured in duplicate at the same sample concentrations. Undiluted samples are as follows : human brain cortex tissue extract 90 µg/mL, mouse whole brain tissue extract 90 µg/mL, and rat whole brain tissue extract 90 µg/mL.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 11 pg/mL

Range

43.75 - 2800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB134130

Anti-CLSTN1 antibody [EPR2963]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CLSTN1 antibody [EPR2963] (AB134130)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CLSTN1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CLSTN1 protein in Cell Lysate, Tissue extracts. Quantitate Human CLSTN1 with 11 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "81 - 96 %", "average": "= 86" }, { "sample": "Tissue Extracts", "range": "92 - 99 %", "average": "= 96" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab316260-96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB316260-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB316260-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB316260-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CLSTN1 Capture Antibody", "number":"AB316260-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CLSTN1 Lyophilized Recombinant Protein", "number":"AB316260-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB316260-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB316260-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB316260-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB316260-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB316260-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CLSTN1 Detector Antibody", "number":"AB316260-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB316260-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Postsynaptic adhesion molecule that binds to presynaptic neurexins to mediate both excitatory and inhibitory synapse formation (By similarity). Promotes synapse development by acting as a cell adhesion molecule at the postsynaptic membrane, which associates with neurexin-alpha at the presynaptic membrane (By similarity). Also functions as a cargo in axonal anterograde transport by acting as a molecular adapter that promotes KLC1 association with vesicles (PubMed : 21385839). Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation (PubMed : 12972431).. Soluble Alc-alpha. As intracellular fragment AlcICD, suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding (PubMed : 15037614).. CTF1-alpha. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP (PubMed : 15037614).
See full target information CLSTN1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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