AB264608

Human CNTF ELISA Kit

Recombinant
SimpleStep
What is this?

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(1 Publication)

Human CNTF ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CNTF with a sensitivity of 6.42 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Ciliary neurotrophic factor, CNTF

5 Images

ELISA

Supplier Data

ELISA - Human CNTF ELISA Kit (AB264608)

sELISA

Supplier Data

Sandwich ELISA - Human CNTF ELISA Kit (AB264608)

Interpolated concentrations of spiked CNTF in human serum, plasma and cell culture supernatant samples.

The concentrations of CNTF were measured in duplicates, interpolated from the CNTF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, plasma (heparin) 12.5% and cell culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human CNTF ELISA Kit (AB264608)

Example of human CNTF standard curve in 1X Sample Diluent PTR.

The CNTF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human CNTF ELISA Kit (AB264608)

Example of human CNTF standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human CNTF ELISA Kit (AB264608)

Recombinant CNTF was spiked into the following species samples and diluted in a 2-fold dilution series in 1X Sample Diluent PTR in 96-well vs. 384-well plates. Undiluted samples are 12.5% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 6.42 pg/mL

Range

46.88 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CNTF ELISA Kit ab264608 is a rapid single-wash 90-min Sandwich ELISA to measure Human CNTF in cell culture media, CSF, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 6.42 pg/mL.

How the assay works

Human CNTF SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CNTF ELISA Kit ab264608 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CNTF antibody [EPR22450-107] - BSA and Azide free (Capture) ab267430
- Anti-CNTF antibody [EPR22450-164] - BSA and Azide free (Detector) ab267430

Human Ciliary neurotrophic factor (CNTF) is a single chain polypeptide of 22.7 kDa. CNTF has been found to be a member of a limited family of neuropoietic cytokines that also includes Leukemia Inhibitory Factor and Oncostatin M. This family of cytokines are characterized by four distinctive ?-helical folds commonly associated with growth hormone. CNTF has been hypothesized to be a target-derived differentiation factor for developing neurons and glial cells. CNTF that is retained intracellularly has been suggested to be a lesion-associated molecule, providing damaged neurons with trophic or survival support following injury. Human CNTF shows 76.6% and 77.4% sequence homology with mouse and rat, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]

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Recovery

[ { "sample": "Cerebral Spinal Fluid", "range": "99 - 110 %", "average": "= 105" }, { "sample": "Serum", "range": "85 - 94 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "87 - 105 %", "average": "= 93" }, { "sample": "Cell culture media", "range": "99 - 105 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "89 - 97 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "90 - 92 %", "average": "= 91" } ]

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What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab264608-1x384Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human CNTF Capture Antibody", "number":"AB264608-CMP01" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB264608-CMP14" }, { "size":"1 x 600 µL", "name":"10X Human CNTF Detector Antibody", "number":"AB264608-CMP02" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264608-CMP11" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB264608-CMP10" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264608-CMP12" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB264608-CMP07" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB264608-CMP08" }, { "size":"1 x 5 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB264608-CMP09" }, { "size":"2 x 1 Vial", "name":"Human CNTF Lyophilized Recombinant Protein", "number":"AB264608-CMP05" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB264608-CMP15" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264608-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264608-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB264608-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264608-CMP13", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264608-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CNTF Lyophilized Recombinant Protein", "number":"AB264608-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CNTF Capture Antibody", "number":"AB264608-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB264608-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB264608-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CNTF Detector Antibody", "number":"AB264608-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264608-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264608-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264608-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

See full target information CNTF

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Stem cells international 2022:2744789 PubMed36106176

2022

Promoting Immortalized Adipose-Derived Stem Cell Transdifferentiation and Proliferation into Neuronal-Like Cells through Consecutive 525 nm and 825 nm Photobiomodulation.

Applications

Unspecified application

Species

Unspecified reactive species

Madeleen Jansen van Rensburg,Anine Crous,Heidi Abrahamse

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CNTF ELISA Kit

ELISA - Human CNTF ELISA Kit (AB264608)

Sandwich ELISA - Human CNTF ELISA Kit (AB264608)

Sandwich ELISA - Human CNTF ELISA Kit (AB264608)

Sandwich ELISA - Human CNTF ELISA Kit (AB264608)

Sandwich ELISA - Human CNTF ELISA Kit (AB264608)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

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