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AB99994

Human CNTF ELISA Kit

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(1 Publication)

Human CNTF ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CNTF in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Ciliary neurotrophic factor, CNTF

2 Images
Sandwich ELISA - Human CNTF ELISA Kit (AB99994)
  • sELISA

Supplier Data

Sandwich ELISA - Human CNTF ELISA Kit (AB99994)

Representative Standard Curve using ab99994

Sandwich ELISA - Human CNTF ELISA Kit (AB99994)
  • sELISA

Supplier Data

Sandwich ELISA - Human CNTF ELISA Kit (AB99994)

Representative Standard Curve using ab99994

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 0.2 ng/mL

Range

0.823 - 200 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CNTF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CNTF in plasma and cell culture supernatants. (Human CNTF concentration is pretty low in normal plasma, it may not be detected in this assay).

This assay employs an antibody specific for Human CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect endogenous Human CNTF in normal serum with ab99994, only in serum spiked with Human CNTF.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 104 %", "average": "= 95.45" }, { "sample": "Plasma", "range": "83 - 104 %", "average": "= 93.28" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99994-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99994-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99994-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB99994-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CNTF Microplate (12 strips x 8 wells)", "number":"AB99994-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB99994-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99994-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human CNTF Standard (lyophilized)", "number":"AB99994-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human CNTF", "number":"AB99994-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB99994-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
See full target information CNTF
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Stem cells (Dayton, Ohio) 36:844-855 PubMed29476565

2018

Human Periodontal Ligament-Derived Stem Cells Promote Retinal Ganglion Cell Survival and Axon Regeneration After Optic Nerve Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Ping Cen,Tsz Kin Ng,Jia-Jian Liang,Xi Zhuang,Xiaowu Yao,Gary Hin-Fai Yam,Haoyu Chen,Herman S Cheung,Mingzhi Zhang,Chi Pui Pang
View all publications
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Product promise

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