Human CNTF ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CNTF in Human in Plasma, Cell culture supernatant samples.
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- Stem cells (Dayton, Ohio) 36:844-8552018Human Periodontal Ligament-Derived Stem Cells Promote Retinal Ganglion Cell Survival and Axon Regeneration After Optic Nerve Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing-Ping Cen et. al.PubMed 29476565
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.823 - 200 ng/mL
- Sensitivity
- < 0.2 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
Alternative names
Ciliary neurotrophic factor, CNTF
What's included?
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Human CNTF ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CNTF in Human in Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.823 - 200 ng/mL
- Assay Platform
- Microplate
- Sensitivity
- < 0.2 ng/mL
- General recovery
- > 92%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 95.45 | Range 84 - 104 % |
Sample type Plasma | Average % = 93.28 | Range 83 - 104 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's CNTF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CNTF in plasma and cell culture supernatants. (Human CNTF concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human CNTF in normal serum with ab99994, only in serum spiked with Human CNTF.
Optimization may be required with urine samples.
Product promise
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2 product images
Sandwich ELISA - Human CNTF ELISA Kit (ab99994)
Representative Standard Curve using ab99994
Sandwich ELISA - Human CNTF ELISA Kit (ab99994)
Representative Standard Curve using ab99994
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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