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AB314721

Human CNTFR alpha ELISA Kit

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Human CNTFR alpha ELISA Kit is a Sandwich ELISA for the measurement of Human CNTFR alpha in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Ciliary neurotrophic factor receptor subunit alpha, CNTF receptor subunit alpha, CNTFR-alpha, CNTFR

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Sandwich ELISA - Human CNTFR alpha ELISA Kit (AB314721)
  • sELISA

Supplier Data

Sandwich ELISA - Human CNTFR alpha ELISA Kit (AB314721)

Example of standard curve using ab314721. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 12 pg/mL

Range

12 - 3000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB305245

Anti-CNTFR antibody [EPR24672-25]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CNTFR antibody [EPR24672-25] (AB305245)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CNTFR alpha ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CNTFR alpha in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CNTFR alpha coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human 4-1BB Ligand (TNFSF9), Activin RIIB, Aminopeptidase P2 (XPNPEP2), BAMBI (NMA), BOC, Brevican, Carbonic Anhydrase XII, Carboxypeptidase A2, CD300c (LMIR2), CD320 (TCblR, 8D6A), CDNF, CDO, CHST1, CHST4, CILP-1, CRIM1, CRTAC1, CXADR, Dopa Decarboxylase (DDC), DPPII (QPP, DPP7), DSPG3, EMR2, FCAR (CD89), FCRL1 (FcRH1), FCRL2 (FcRH2), Gas6, GPR56, GPVI, Hepsin, ILT2 (CD85j), Jagged 2, Kirrel3 (NEPH2), KLF4, LAIR1, LAMP, LAMP1 (CD107a), MDGA1, MIS RII, Neurexin 3.

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Recovery

[ { "sample": "Serum", "range": "101 - 110 %", "average": "= 105.3" }, { "sample": "Plasma", "range": "103 - 125 %", "average": "= 113.2" }, { "sample": "Cell culture media", "range": "115 - 133 %", "average": "= 123" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314721-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314721-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human CNTFR alpha Protein Standard", "number":"AB314721-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human CNTFR alpha Detection Antibody", "number":"AB314721-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human CNTFR alpha Antibody-coated ELISA Plate", "number":"AB314721-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314721-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314721-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB314721-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314721-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Binds to CNTF. The alpha subunit provides the receptor specificity. Receptor for heterodimeric neurotropic cytokine composed of CLCF1/CLC and CRLF1/CLF-1 (PubMed : 26858303). Acts as a receptor for the neuroprotective peptide humanin as part of a complex with IL6ST/GP130 and IL27RA/WSX1 (PubMed : 19386761).
See full target information CNTFR
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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