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AB323922

Human Cofilin-1 ELISA Kit

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Human Cofilin-1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Cofilin-1 in Human in Cell/Tissue Extracts samples.

View Alternative Names

CFL, CFL1, Cofilin-1, 18 kDa phosphoprotein, p18

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Sandwich ELISA - Human Cofilin-1 ELISA Kit (AB323922)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cofilin-1 ELISA Kit (AB323922)

Interpolated concentration of native Cofilin-1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : MCF7 cell extract 5 μg/mL, NIH/3T3 cell extract 100 μg/mL, and C6 cell extract 100 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

Sandwich ELISA - Human Cofilin-1 ELISA Kit (AB323922)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cofilin-1 ELISA Kit (AB323922)

Example of human Cofilin-1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Cofilin-1 ELISA Kit (AB323922)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cofilin-1 ELISA Kit (AB323922)

Interpolated concentration of native Cofilin-1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100 μg/mL C6 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

Sandwich ELISA - Human Cofilin-1 ELISA Kit (AB323922)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cofilin-1 ELISA Kit (AB323922)

Example of human Cofilin-1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 60.63 pg/mL

Range

218.75 - 14000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Cofilin-1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cofilin-1 protein in Cell Lysate. Quantitate Human Cofilin-1 with 60.63 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

Singlewash protocol reduces assay time to 90 minutes or less.

High sensitivity, specificity and reproducibility from superior antibodies.

Fully validated in biological samples.

96wells plate breakable into 12 x 8 wells strips, available in 11pack (10 x 96well plates).

Also available in a fully validated 384well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.6" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.3" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "97 - 115 %", "average": "= 106" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed : 11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed : 15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed : 21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed : 23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).
See full target information CFL1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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