Human Cofilin-1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Cofilin-1 in Cell Lysate, Tissue Extracts samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 218.75 - 14000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 60.63 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).
Alternative names
CFL, CFL1, Cofilin-1, 18 kDa phosphoprotein, p18
What's included?
Human Cofilin-1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Cofilin-1 in Cell Lysate, Tissue Extracts samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 218.75 - 14000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 60.63 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 8 | C.V. = 5.6 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. = 2.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 106 | Range 97 - 115 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Cofilin-1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cofilin-1 protein in Cell Lysate. Quantitate Human Cofilin-1 with 60.63 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
Singlewash protocol reduces assay time to 90 minutes or less.
High sensitivity, specificity and reproducibility from superior antibodies.
Fully validated in biological samples.
96wells plate breakable into 12 x 8 wells strips, available in 11pack (10 x 96well plates).
Also available in a fully validated 384well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Sandwich ELISA - Human Cofilin-1 ELISA Kit (ab323922)
Interpolated concentration of native Cofilin-1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100 μg/mL C6 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
Sandwich ELISA - Human Cofilin-1 ELISA Kit (ab323922)
Interpolated concentration of native Cofilin-1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: MCF7 cell extract 5 μg/mL, NIH/3T3 cell extract 100 μg/mL, and C6 cell extract 100 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
Sandwich ELISA - Human Cofilin-1 ELISA Kit (ab323922)
Example of human Cofilin-1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Cofilin-1 ELISA Kit (ab323922)
Example of human Cofilin-1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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