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AB288579

Human COLEC11 ELISA Kit

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Human COLEC11 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human COLEC11 with a sensitivity of 0.09 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

UNQ596/PRO1182, COLEC11, Collectin-11, Collectin kidney protein 1, CL-K1

3 Images
Sandwich ELISA - Human COLEC11 ELISA Kit (AB288579)
  • sELISA

Supplier Data

Sandwich ELISA - Human COLEC11 ELISA Kit (AB288579)

Interpolated concentration of native COLEC11 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 100 serum, plasma (citrate), plasma (heparin) and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human COLEC11 ELISA Kit (AB288579)
  • sELISA

Supplier Data

Sandwich ELISA - Human COLEC11 ELISA Kit (AB288579)

Serum of nine individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean COLEC11 concentration was determined to be 0.85 μg/mL with a range of 0.61 - 1.05 μg/mL.

Sandwich ELISA - Human COLEC11 ELISA Kit (AB288579)
  • sELISA

Supplier Data

Sandwich ELISA - Human COLEC11 ELISA Kit (AB288579)

Example of human COLEC11 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.09 ng/mL

Range

0.266 - 17 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human COLEC11 ELISA Kit (ab288579) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of COLEC11 protein in a variety of samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human COLEC11 with 0.09 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.9" } ]
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Recovery

[ { "sample": "Serum", "range": "101 - 107 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "100 - 103 %", "average": "= 102" }, { "sample": "Cell culture media", "range": "89 - 94 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "91 - 101 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "97 - 106 %", "average": "= 102" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288579-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB288579-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB288579-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288579-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human COLEC11 Lyophilized Recombinant Protein", "number":"AB288579-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288579-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human COLEC11 Capture Antibody", "number":"AB288579-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB288579-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB288579-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB288579-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human COLEC11 Detector Antibody", "number":"AB288579-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Lectin that plays a role in innate immunity, apoptosis and embryogenesis (PubMed : 21258343, PubMed : 23954398, PubMed : 25912189). Calcium-dependent lectin that binds self and non-self glycoproteins presenting high mannose oligosaccharides with at least one terminal alpha-1,2-linked mannose epitope (PubMed : 25912189). Primarily recognizes the terminal disaccharide of the glycan (PubMed : 25912189). Also recognizes a subset of fucosylated glycans and lipopolysaccharides (PubMed : 17179669, PubMed : 25912189). Plays a role in innate immunity through its ability to bind non-self sugars presented by microorganisms and to activate the complement through the recruitment of MAPS1 (PubMed : 20956340, PubMed : 25912189). Also plays a role in apoptosis through its ability to bind in a calcium-independent manner the DNA present at the surface of apoptotic cells and to activate the complement in response to this binding (Probable). Finally, plays a role in development, probably serving as a guidance cue during the migration of neural crest cells and other cell types during embryogenesis (PubMed : 21258343, PubMed : 28301481).
See full target information COLEC11
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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