AB230937

Human Colipase ELISA Kit

Recombinant
SimpleStep
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Human Colipase ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Colipase with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Colipase, CLPS

5 Images

Sandwich ELISA - Human Colipase ELISA Kit (AB230937)

sELISA

Supplier Data

Sandwich ELISA - Human Colipase ELISA Kit (AB230937)

Example of human Colipase standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Colipase ELISA Kit (AB230937)

Example of human Colipase standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Colipase ELISA Kit (AB230937)

Interpolated concentrations of native Colipase in human serum, plasma, and urine samples.

The concentrations of Colipase were measured in duplicates, interpolated from the Colipase standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 3.1%, plasma (heparin) 10%, plasma (EDTA) 6.3%, and urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Colipase concentration was determined to be 2684 pg/mL in serum, 5049 pg/mL in plasma (citrate), 2526 pg/ml in plasma (heparin), 3666 pg/ml in plasma (EDTA), and 613 pg/mL in urine.

sELISA

Supplier Data

Sandwich ELISA - Human Colipase ELISA Kit (AB230937)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Colipase concentration was determined to be 3348 pg/mL with a range of 1229 – 5295 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Colipase ELISA Kit (AB230937)

Interpolated concentrations of native Colipase in human pancreas homogenate extract based on a 0.04 μg/mL extract load.

The concentrations of Colipase were measured in duplicate and interpolated from the Colipase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Colipase concentration was determined to be 243 pg/mL in human pancreas homogenate extract at this loading.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Tissue Homogenate, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 3 pg/mL

Range

5 - 300 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Colipase ELISA Kit (ab230937) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Colipase protein in cit plasma, edta plasma, hep plasma, serum, tissue homogenate, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Colipase with 3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "EDTA Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.1" }, { "reproducibilityType": "Intra", "sample": "EDTA Plasma", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "102 - 104 %", "average": "= 103" }, { "sample": "Serum", "range": "97 - 99 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "104 - 124 %", "average": "= 111" }, { "sample": "Urine", "range": "86 - 94 %", "average": "= 90" }, { "sample": "Heparin Plasma", "range": "101 - 121 %", "average": "= 108" }, { "sample": "Tissue Homogenate", "range": "100 - 103 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "104 - 120 %", "average": "= 111" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab230937-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB230937-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB230937-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB230937-CMP08", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB230937-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB230937-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB230937-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Colipase Capture Antibody", "number":"AB230937-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB230937-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Colipase Lyophilized Recombinant Protein", "number":"AB230937-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Colipase Detector Antibody", "number":"AB230937-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB230937-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB230937-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Colipase is a cofactor of pancreatic lipase. It allows the lipase to anchor itself to the lipid-water interface. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase.. Enterostatin has a biological activity as a satiety signal.

See full target information Colipase

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Colipase ELISA Kit

Sandwich ELISA - Human Colipase ELISA Kit (AB230937)

Sandwich ELISA - Human Colipase ELISA Kit (AB230937)

Sandwich ELISA - Human Colipase ELISA Kit (AB230937)

Sandwich ELISA - Human Colipase ELISA Kit (AB230937)

Sandwich ELISA - Human Colipase ELISA Kit (AB230937)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

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Primary Antibodies

Assay Kits

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Product promise