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AB285250

Human Collagen Type I ELISA Kit

4

(1 Review)

|

(4 Publications)

Human Collagen Type I ELISA kit (colorimetric) is a sandwich ELISA designed to quantify human collagen type I (COL1A1) with a sensitivity of 0.188 ng/mL.

- Wide dynamic range – quantifies 0.313–20 ng/mL
- Compatible with multiple sample types – works with serum, plasma, tissue homogenates, cell culture supernatants, cell lysates, and other biological fluids
- Assay platform – microplate (12 x 8 well strips) for easy setup and reliable results

View Alternative Names

Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1

1 Images
ELISA - Human Collagen Type I ELISA Kit (AB285250)
  • ELISA

Supplier Data

ELISA - Human Collagen Type I ELISA Kit (AB285250)

Collagen Type I (Human) Elisa Kit Standard Curve.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Serum, Other biological fluids, Cell culture supernatant, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Sensitivity

< 0.188 ng/mL

Range

0.313 - 20 ng/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Collagen Type I ELISA Kit ab285250 is a sandwich ELISA kit to measure human collagen type I (COL1A1) with a sensitivity of 0.188 ng/mL.

How the assay works

Human Collagen Type I ELISA Kit ab285250 uses a pair of antibodies each specific to different epitopes at the target of interest: the capture antibody is coated on the surface of the multi-well plate to facilitate the immobilization of the antigen. The other antibody is conjugated and facilitates the detection of the antigen.

Assay specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.

Human Collagen Type I ELISA Kit ab285250 protocol summary

1. Add standards or samples to the wells, incubate 2 hrs

2. Wash, then add 50 µl of Biotinylated Antibody per well. Incubate 1 hr

3. Wash, then add 50 µl of SP Conjugate per well. Incubate 30 min

4. Wash, then add 50 µl of Chromogen Substrate per well. Incubate 20 min

5. Add 50 µl of Stop Solution per well. Read at 450 nm immediately

How other researchers are using Human Collagen Type I ELISA Kit ab285250

Human Collagen Type I ELISA Kit ab285250 has been used to study USP37 regulation of c-Myc to promote keloid fibroblast proliferation and collagen production (1).

References:

(1)Guangdong Feng et al. 2023, PMID: 36333840

Related and recommended products

Human Collagen Type I ELISA Kit ab285250 is commonly used to study tissue structure and function.

Other ELISA kits to measure tissue repair include:

- Human Elastin ELISA kit ab239433
- Human Fibronectin ELISA kit ab219046
- Prostaglandin E2 ELISA Kit ab287802
- Dopamine ELISA Kit ab285238

Get results in 90 minutes with our SimpleStep ELISA® kit

Human Pro-Collagen I alpha 1 ELISA Kit ab210966

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
Biological function summary

Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.

Pathways

Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.

Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.
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Product protocols

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Target data

Type I collagen is a member of group I collagen (fibrillar forming collagen).
See full target information COL1A1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:19091 PubMed40447668

2025

Inflammatory factors released by ACL reconstruction tendon grafts and their potential for macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Janne Spierings,Marina van Doeselaar,Mads Buenen,Florencia Abinzano,Marieke van der Steen,Rob Janssen,Keita Ito,Jasper Foolen

ERJ open research 11: PubMed39834596

2025

Role of pulmonary rehabilitation in extracellular matrix protein expression in vastus lateralis muscle in atrophic and nonatrophic patients with COPD.

Applications

Unspecified application

Species

Unspecified reactive species

Efpraxia Kritikaki,Gerasimos Terzis,Meera Soundararajan,Ioannis Vogiatzis,Davina C M Simoes

ERJ open research 10: PubMed38803416

2024

Expression of intramuscular extracellular matrix proteins in vastus lateralis muscle fibres between atrophic and non-atrophic COPD.

Applications

Unspecified application

Species

Unspecified reactive species

Efpraxia Kritikaki,Gerasimos Terzis,Meera Soundararajan,Ioannis Vogiatzis,Davina C M Simoes

International wound journal 20:1517-1524 PubMed36333840

2022

The deubiquitinating enzyme USP37 promotes keloid fibroblasts proliferation and collagen production by regulating the c-Myc expression.

Applications

Unspecified application

Species

Unspecified reactive species

Meishan Piao,Guangdong Feng
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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