Human Complement C1 ELISA Kit

The Human Complement C1 ELISA (Enzyme-Linked Immunosorbent Assay) Kit (an222865) is designed for detection of C1 in human plasma, serum, saliva, urine, milk, and cell culture samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures C1 in less than 4 hours. A polyclonal antibody specific for C1 has been pre-coated onto a 96-well microplate with removable strips. C1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for C1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Complement component C1 (C1) is a calcium-dependent serine protease complex with an approximate mass of 790 kDa and acts as the first component of the classical complement pathway. C1 is formed from the association of a recognition protein C1q and two catalytic subunits C1r and C1s respectively. The globular heads of the C1q bind to the Fc-fragment of IgM or IgG on the surface of a pathogen, resulting in the activation of C1r. The activated C1r is able to activate C1s which in turn activates C2 and C4, leading to the production of the C4b-C2a form of C3-convertase.