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AB170245

Human Complement C1r ELISA Kit

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(1 Publication)

Human Complement C1r ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Complement C1r in Human in Biofluids samples.

View Alternative Names

Complement C1r subcomponent, Complement component 1 subcomponent r, C1R

1 Images
Sandwich ELISA - Human Complement C1r ELISA Kit (AB170245)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement C1r ELISA Kit (AB170245)

Representative Standard Curve using ab170245

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Saliva, Urine, Plasma, Milk, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 92 pg/mL

Range

0.125 - 8 ng/mL

Assay time

4h

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Complement C1r Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C1r concentrations in plasma, serum, saliva, urine, milk and cell culture supernatants.

A Complement C1r specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C1r specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C1r captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab170245-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"60X Biotinylated Human Complement C1r Antibody", "number":"AB170245-CMP01", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB170245-CMP06", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB170245-CMP08", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB170245-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Complement C1r Standard", "number":"AB170245-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent M Concentrate", "number":"AB170245-CMP02", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB170245-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Complement C1r Microplate (12 x 8 well strips)", "number":"AB170245-CMP04", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB170245-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.
See full target information C1R
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40277901

2025

Investigation on ABCC6-Deficient Human Hepatocytes Generated by CRISPR-Cas9 Genome Editing.

Applications

Unspecified application

Species

Unspecified reactive species

Ricarda Plümers,Svenja Jelinek,Christopher Lindenkamp,Michel R Osterhage,Cornelius Knabbe,Doris Hendig
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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