Human Complement C2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Complement C2 in Human in Cerebral Spinal Fluid, Plasma, Milk, Serum samples.
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- Journal of clinical immunology 41:1607-16202021Elevated Expression Levels of Lung Complement Anaphylatoxin, Neutrophil Chemoattractant Chemokine IL-8, and RANTES in MERS-CoV-Infected Patients: Predictive Biomarkers for Disease Severity and Mortality.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaaweya E Hamed,Asif Naeem,Haitham Alkadi,Aref A Alamri,Ahmad S AlYami,Abdullah AlJuryyan,Wael Alturaiki,Mushira Enani,Samia T Al-Shouli,Abdullah M Assiri,Bandar AlosaimiPubMed 34232441
- Frontiers in immunology 12:6687252021Complement Anaphylatoxins and Inflammatory Cytokines as Prognostic Markers for COVID-19 Severity and In-Hospital Mortality.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBandar Alosaimi,Ayman Mubarak,Maaweya E Hamed,Abdullah Z Almutairi,Ahmed A Alrashed,Abdullah AlJuryyan,Mushira Enani,Faris Q Alenzi,Wael AlturaikiPubMed 34276659
- Cell 168:1101-1113.e132017Complement Component 3 Adapts the Cerebrospinal Fluid for Leptomeningeal Metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrienne Boire,Yilong Zou,Jason Shieh,Danilo G Macalinao,Elena Pentsova,Joan MassaguéPubMed 28283064
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Plasma, Milk, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.00625 - 0.4 µg/mL
- Assay time
- 4h
- Sensitivity
- = 0.0032 µg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase.
Alternative names
Complement C2, C3/C5 convertase, C2
What's included?
Recommended products
Human Complement C2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Complement C2 in Human in Cerebral Spinal Fluid, Plasma, Milk, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Plasma, Milk, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.00625 - 0.4 µg/mL
- Assay time
- 4h
- Assay Platform
- Microplate
- Sensitivity
- = 0.0032 µg/mL
- General recovery
- 97%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 9.8 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's Complement C2 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C2 in plasma, serum, milk, saliva, CSF, cell culture supernatants, cell lysate, and tissue samples.
A Complement C2 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C2 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C2 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sandwich ELISA - Human Complement C2 ELISA Kit (ab154132)
Representative Standard Curve using ab154132
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Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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