JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB154132

Human Complement C2 ELISA Kit

3

(1 Review)

|

(3 Publications)

Human Complement C2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Complement C2 in Human in Biofluids samples.

View Alternative Names

Complement C2, C3/C5 convertase, C2

1 Images
Sandwich ELISA - Human Complement C2 ELISA Kit (AB154132)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement C2 ELISA Kit (AB154132)

Representative Standard Curve using ab154132

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Plasma, Milk, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

>= 2.5 ng/mL

Range

6.25 - 400 ng/mL

Assay time

4h

Assay Platform

Microplate

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's Complement C2 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C2 in plasma, serum, milk and CSF samples.

A Complement C2 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C2 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C2 captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab154132-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB154132-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Complement C2 Antibody", "number":"AB154132-CMP01", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB154132-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Complement C2 Standard", "number":"AB154132-CMP05", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB154132-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Complement C2 Microplate (12 x 8 well strips)", "number":"AB154132-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB154132-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB154132-CMP02", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB154132-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments : C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase.
See full target information C2
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical immunology 41:1607-1620 PubMed34232441

2021

Elevated Expression Levels of Lung Complement Anaphylatoxin, Neutrophil Chemoattractant Chemokine IL-8, and RANTES in MERS-CoV-Infected Patients: Predictive Biomarkers for Disease Severity and Mortality.

Applications

Unspecified application

Species

Unspecified reactive species

Maaweya E Hamed,Asif Naeem,Haitham Alkadi,Aref A Alamri,Ahmad S AlYami,Abdullah AlJuryyan,Wael Alturaiki,Mushira Enani,Samia T Al-Shouli,Abdullah M Assiri,Bandar Alosaimi

Frontiers in immunology 12:668725 PubMed34276659

2021

Complement Anaphylatoxins and Inflammatory Cytokines as Prognostic Markers for COVID-19 Severity and In-Hospital Mortality.

Applications

Unspecified application

Species

Unspecified reactive species

Bandar Alosaimi,Ayman Mubarak,Maaweya E Hamed,Abdullah Z Almutairi,Ahmed A Alrashed,Abdullah AlJuryyan,Mushira Enani,Faris Q Alenzi,Wael Alturaiki

Cell 168:1101-1113.e13 PubMed28283064

2017

Complement Component 3 Adapts the Cerebrospinal Fluid for Leptomeningeal Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Adrienne Boire,Yilong Zou,Jason Shieh,Danilo G Macalinao,Elena Pentsova,Joan Massagué
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB300318

Human Factor B ELISA Kit

Recombinant|SimpleStep

ELISA - Human Factor B ELISA Kit (AB300318)

  • 4
  • 1
Assay Kits

AB125963

Human Complement C5 ELISA Kit

Sandwich ELISA - Human Complement C5 ELISA Kit (AB125963)

  • 0
  • 0
Assay Kits

AB287178

Human C9 ELISA Kit (Complement C9)

Recombinant|SimpleStep

Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)

  • 0
  • 0
Assay Kits

AB284615

Human Complement Factor I ELISA Kit

Recombinant|SimpleStep

ELISA - Human Complement Factor I ELISA Kit (AB284615)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com