Human Complement C2 ELISA Kit
- Recombinant
- SimpleStep
- What is this?
Be the first to review this product! Submit a review
|
(0 Publication)
Human Complement C2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Complement C2 with a sensitivity of 175.11 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Complement C2, C3/C5 convertase, C2
- ELISA
Supplier Data
ELISA - Human Complement C2 ELISA Kit (AB254501)
- sELISA
Supplier Data
Sandwich ELISA - Human Complement C2 ELISA Kit (AB254501)
Interpolated concentrations of native Complement C2 in human cell culture supernatant, milk, and female saliva samples.
The concentrations of Complement C2 were measured in duplicates, interpolated from the Complement C2 standard curves and corrected for sample dilution. Undiluted samples are as follows : HepG2 cell culture supernatant 6.25%, milk 12.5%, and female saliva 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C2 concentration was determined to be 117.47 ng/mL in HepG2 cell culture supernatant, 117.91 ng/mL in milk, and 22.87 ng/mL in female saliva.
- sELISA
Supplier Data
Sandwich ELISA - Human Complement C2 ELISA Kit (AB254501)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C2 concentration was determined to be 19.67 μg/mL with a range of 12.10 – 27.26 μg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Complement C2 ELISA Kit (AB254501)
Interpolated concentrations of native Complement C2 in human serum and plasma samples.
The concentrations of Complement C2 were measured in duplicates, interpolated from the Complement C2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 4,000, plasma (citrate) 1 : 2,000, plasma (heparin) 1 : 1,000, and plasma (EDTA) 1 : 1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C2 concentration was determined to be 27.00 μg/mL in serum, 15.46 μg/mL in plasma (citrate), 11.89 μg/ml in plasma (heparin), and 12.51 μg/ml in plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Human Complement C2 ELISA Kit (AB254501)
Example of human Complement C2 standard curve in Sample Diluent NS.
The Complement C2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Complementary Products
Reactivity data
Product details
Human Complement C2 ELISA Kit ab254501 is a rapid single-wash 90-min Sandwich ELISA to measure Human Complement C2 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, milk, saliva, serum. This SimpleStep sensitivity is 175.11 pg/mL.
How the assay works
Human Complement C2 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Complement C2 ELISA Kit ab254501 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-C2 antibody [EPR22452-69] - BSA and Azide free (Capture) ab259713
- Anti-C2 antibody [EPR22452-177] - BSA and Azide free (Detector) ab259714
Complement C2 is a serine protease involved in both the classical and lectin pathways of complement activation. C2 is secreted as a zymogen. Activation of C2 by C1 leads to the proteolysis of C2 into C2a and C2b. Deficiency of C2 can lead to various types of autoimmune diseases.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com