Human Complement C3 ELISA Kit

Product details

Human Complement C3 ELISA kit is designed for the quantitative measurement of Complement C3 concentrations in Human urine, milk, saliva, cerebrospinal fluid and cell culture supernatants.

A Complement C3 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C3 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C3 captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Human Complement C3 ELISA Kit ab108823 is a sandwich ELISA to measure Human complement C3 in urine, milk, saliva, cerebrospinal fluid and cell culture supernatants with a sensitivity of 84 pg/mL.

How the assay works

A Complement C3 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C3 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C3 captured in plate.

Assay specificity

This kit recognizes Complement C3 in urine, milk, saliva, cerebrospinal fluid, tissue and cell culture supernatant

Human Complement C3 ELISA Kit ab108823 protocol summary

1. Add samples and standards to wells. Incubate at room temperature.

2. Wash each well and add enzyme-antibody conjugate. Incubate at room temperature.

3. Wash each well and add Streptavidin Solution. Incubate at room temperature.

4. Add TMB Solution to each well. Incubate at room temperature.

5. Add Stop Solution to each well. Read immediately.

How other researchers are using Human C3 ELISA kit ab108823

Human C3 ELISA kit ab108823 has been used in the study of the relationsip between microglia and complement in Huntingdon's disease ⁽¹⁾and to assess the function of kidney organoids ⁽²⁾.

References:

⁽¹⁾: DK Wilton et al. 2023, PMID: 37814059,
⁽²⁾M Lasse et al., 2023,PMID: 37580326

Related and recommended products

Human Complement C3 Elisa kit ab108823 is commonly used to assess the ability of the immune system to fight infection.

Other ELISA kits to measure complement factors include:

- Human Complement factor H ELISA Kit ab252359
- Human Complement Factor I ELISA Kit ab284615
- Human C4b ELISA Kit (Complement C4-B) ab277717
- Human C8a (Complement C8a) ELISA Kit ab288173
- Human Complement Factor D ELISA Kit ab243686