Human Complement C3 ELISA Kit ab108823 is used to quantify Human Complement C3 with a sensitivity of 0.32 ng/mL.
Sandwich ELISA Kit
- Validated in a range of biological samples including CSF
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- Nature medicine 29:2866-28842023Microglia and complement mediate early corticostriatal synapse loss and cognitive dysfunction in Huntington's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel K Wilton,Kevin Mastro,Molly D Heller,Frederick W Gergits,Carly Rose Willing,Jaclyn B Fahey,Arnaud Frouin,Anthony Daggett,Xiaofeng Gu,Yejin A Kim,Richard L M Faull,Suman Jayadev,Ted Yednock,X William Yang,Beth StevensPubMed 37814059
- Nature communications 14:49032023An integrated organoid omics map extends modeling potential of kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMoritz Lassé,Jamal El Saghir,Celine C Berthier,Sean Eddy,Matthew Fischer,Sandra D Laufer,Dominik Kylies,Arvid Hutzfeldt,Léna Lydie Bonin,Bernhard Dumoulin,Rajasree Menon,Virginia Vega-Warner,Felix Eichinger,Fadhl Alakwaa,Damian Fermin,Anja M Billing,Akihiro Minakawa,Phillip J McCown,Michael P Rose,Bradley Godfrey,Elisabeth Meister,Thorsten Wiech,Mercedes Noriega,Maria Chrysopoulou,Paul Brandts,Wenjun Ju,Linda Reinhard,Elion Hoxha,Florian Grahammer,Maja T Lindenmeyer,Tobias B Huber,Hartmut Schlüter,Steffen Thiel,Laura H Mariani,Victor G Puelles,Fabian Braun,Matthias Kretzler,Fatih Demir,Jennifer L Harder,Markus M RinschenPubMed 37580326
- International journal of molecular sciences 24:2023Anti-Spike Antibodies Present in the Milk of SARS-CoV-2 Vaccinated Mothers Are Complement-Activating.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiara Agostinis,Miriam Toffoli,Andrea Balduit,Alessandro Mangogna,Hadida Yasmin,Chiara Ragazzon,Silvia Pegoraro,Giuseppina Campisciano,Guglielmo Stabile,Gabriella Zito,Uday Kishore,Manola Comar,Federica Scrimin,Roberta Bulla,Giuseppe RicciPubMed 36901824
- BMC psychiatry 22:7412022Plasma complement C3 and C3a are increased in major depressive disorder independent of childhood trauma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianmei Luo,Zeman Fang,Lingyun Lin,Haiyun Xu,Qingjun Huang,Handi ZhangPubMed 36447174
- Egyptian liver journal 12:672022Serum cystatin C is an early renal dysfunction biomarker in patients with hepatitis C virus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNagwa Mohamed Assem,Amany Ibrahim Mohammed,Hamed Mohamed Abdel Barry,Ibrahim El Tantawy El Sayed,Ibrahim ElmadbouhPubMed 36466932
- Frontiers in immunology 13:8615452022Human Melanoma-Associated Mast Cells Display a Distinct Transcriptional Signature Characterized by an Upregulation of the Complement Component 3 That Correlates With Poor Prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRajia Bahri,Orsolya Kiss,Ian Prise,Karen M Garcia-Rodriguez,Haris Atmoko,Julia M Martínez-Gómez,Mitchell P Levesque,Reinhard Dummer,Michael P Smith,Claudia Wellbrock,Silvia Bulfone-PausPubMed 35669782
- Frontiers in cellular and infection microbiology 11:7619452021An Immunomodulatory Transcriptional Signature Associated With Persistent Infection in Hepatocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalie Descoeudres,Luc Jouneau,Céline Henry,Kevin Gorrichon,Aurélie Derré-Bobillot,Pascale Serror,Laura Lee Gillespie,Cristel Archambaud,Alessandro Pagliuso,Hélène BiernePubMed 34858876
- Journal of affective disorders 286:275-2812021Evidence of upregulation of the cholinergic anti-inflammatory pathway in late-life depression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNunzio Pomara,Davide Bruno,Chelsea Reichert Plaska,Anilkumar Pillai,Jaime Ramos-Cejudo,Ricardo Osorio,Bruno P Imbimbo,Amanda Heslegrave,Henrik Zetterberg,Kaj BlennowPubMed 33756305
- ACS biomaterials science & engineering 6:4903-49152020Development of a Sensitive Assay to Screen Nanoparticles for Complement Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNuzhat Maisha,Tobias Coombs,Erin LavikPubMed 33313396
- Nature 582:577-5812020Complement genes contribute sex-biased vulnerability in diverse disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNolan Kamitaki,Aswin Sekar,Robert E Handsaker,Heather de Rivera,Katherine Tooley,David L Morris,Kimberly E Taylor,Christopher W Whelan,Philip Tombleson,Loes M Olde Loohuis,Michael Boehnke,Robert P Kimberly,Kenneth M Kaufman,John B Harley,Carl D Langefeld,Christine E Seidman,Michele T Pato,Carlos N Pato,Roel A Ophoff,Robert R Graham,Lindsey A Criswell,Timothy J Vyse,Steven A McCarrollPubMed 32499649
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Cerebral Spinal Fluid, Saliva, Urine, Cell culture supernatant, Milk, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.625 - 40 ng/mL
- Assay time
- 4h
- Sensitivity
- >= 84 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed:10432298, PubMed:15833747, PubMed:16333141, PubMed:19615750, PubMed:2909530, PubMed:8376604, PubMed:9059512).
Alternative names
CPAMD1, C3, Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
What's included?
Recommended products
Human Complement C3 ELISA Kit ab108823 is used to quantify Human Complement C3 with a sensitivity of 0.32 ng/mL.
Sandwich ELISA Kit
- Validated in a range of biological samples including CSF
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Cerebral Spinal Fluid, Saliva, Urine, Cell culture supernatant, Milk, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.625 - 40 ng/mL
- Assay time
- 4h
- Assay Platform
- Microplate
- Sensitivity
- >= 84 pg/mL
- General recovery
- 104%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 10.7 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Human Complement C3 ELISA kit is designed for the quantitative measurement of Complement C3 concentrations in Human urine, milk, saliva, cerebrospinal fluid and cell culture supernatants.
A Complement C3 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C3 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C3 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Human Complement C3 ELISA Kit ab108823 is a sandwich ELISA to measure Human complement C3 in urine, milk, saliva, cerebrospinal fluid and cell culture supernatants with a sensitivity of 84 pg/mL.
How the assay works
A Complement C3 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C3 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C3 captured in plate.
Assay specificity
This kit recognizes Complement C3 in urine, milk, saliva, cerebrospinal fluid, tissue and cell culture supernatant
Human Complement C3 ELISA Kit ab108823 protocol summary
1. Add samples and standards to wells. Incubate at room temperature.
2. Wash each well and add enzyme-antibody conjugate. Incubate at room temperature.
3. Wash each well and add Streptavidin Solution. Incubate at room temperature.
4. Add TMB Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.
How other researchers are using Human C3 ELISA kit ab108823
Human C3 ELISA kit ab108823 has been used in the study of the relationsip between microglia and complement in Huntingdon's disease (1)and to assess the function of kidney organoids (2).
References:
(1): DK Wilton et al. 2023, PMID: 37814059,
(2)M Lasse et al., 2023,PMID: 37580326
Related and recommended products
Human Complement C3 Elisa kit ab108823 is commonly used to assess the ability of the immune system to fight infection.
Other ELISA kits to measure complement factors include:
- Human Complement factor H ELISA Kit Human Complement factor H ELISA Kit ab252359
- Human Complement Factor I ELISA Kit Human Complement Factor I ELISA Kit ab284615
- Human C4b ELISA Kit (Complement C4-B) Human C4b ELISA Kit (Complement C4-B) ab277717
- Human C8a (Complement C8a) ELISA Kit Human C8a (Complement C8a) ELISA Kit ab288173
- Human Complement Factor D ELISA Kit Human Complement Factor D ELISA Kit ab243686
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Complement component 3 (C3) commonly known as C3 complement is a central protein in the complement system which plays a significant role in immune response. C3b a fragment of C3 is produced when C3 undergoes cleavage. C3 is a large protein with a mass of approximately 185 kDa. The liver primarily secretes C3 into the bloodstream. It circulates in the plasma and is found in high concentration making it one of the most abundant components of the complement system.
- Biological function summary
Complement component C3 forms part of the innate immune system by promoting opsonization which enhances phagocytosis of pathogens. C3b binds to pathogens' surfaces facilitating their recognition by phagocytes. C3 as part of a complex with C3 convertase also has a role in amplifying the activation of the complement cascade. The proteolytic cleavage of C3 into C3b and C3a leads to the activation of other components forming the membrane attack complex and orchestrating inflammation.
- Pathways
The complement component C3 functions within both the classical and alternative complement pathways. It acts as a convergence point where the complement activation pathways meet. C3 is activated into C3b and C3a which are key to amplifying the cascade. Furthermore C3 interacts with proteins such as factor B and factor D in the alternative pathway and C4 and C2 in the classical pathway facilitating the formation of C3 convertase necessary for pathway progression.
- Associated diseases and disorders
Complement C3 shows associations with immune-related and inflammatory diseases. Deficiencies or malfunctions of complement C3 can lead to increased susceptibility to infections due to impaired opsonization and clearance of pathogens. Additionally overactivation of the complement system involving C3 can contribute to autoimmune disorders such as systemic lupus erythematosus. Other proteins linked to these diseases include C4 in lupus and factor H in age-related macular degeneration which controls complement pathway activation.
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3 product images
Sandwich ELISA - Human Complement C3 ELISA Kit (ab108823)
Complement C3 measured in various samples showing quantity (microgram) per mL of tested sample
Sandwich ELISA - Human Complement C3 ELISA Kit (ab108823)
Representative Standard Curve using ab108823
Sandwich ELISA - Human Complement C3 ELISA Kit (ab108823)
Representative Standard Curve using ab108823
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