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AB133037

Human Complement C3a des Arg ELISA Kit

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(10 Publications)

Human Complement C3a des Arg ELISA Kit is a Competitive ELISA for the measurement of Human Complement C3a des Arg in Human in Biofluids samples.

View Alternative Names

CPAMD1, C3, Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1

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Competitive ELISA - Human Complement C3a des Arg ELISA Kit (AB133037)
  • cELISA

Supplier Data

Competitive ELISA - Human Complement C3a des Arg ELISA Kit (AB133037)

Representative Standard Curve using ab133037

Key facts

Detection method

Colorimetric

Sample types

Plasma

Reacts with

Human

Assay type

Competitive

Sensitivity

= 0.12 ng/mL

Range

0.313 - 20 ng/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Complement C3a des Arg Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is for the accurate quantitation of Human Complement C3a des Arg in plasma.

A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Complement C3a des Arg antigen and a polyclonal rabbit antibody specific to Complement C3a des Arg. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Complement C3a des Arg captured in the plate.

The Human C3a des Arg molecule is one of three activation fragments formed from the activation of the complement cascade. C3a des Arg is formed from C3a via carboxypeptidase cleavage of the C-terminal arginine group. The structure of Human C3a des Arg was first reported in 1975 by Hugli. Human C3a des Arg contains 77 amino acids with 6 cysteines involved in disulfide bridges between residues 22-49, 23-56 and 36-57. The C terminal end of C3a des Arg in Human, porcine, rat, mouse and guinea pig is identical. C3a des Arg is a highly cationic molecule containing no carbohydrate. X-ray crystal data shows the N and C terminal residues to have highly flexible helical structures. C3a is one of the most potent constrictors of smooth muscle cells, and guinea pig airways are hyperresponsive to C3a when pretreated with histamine. The long term study of liver and other transplant recipients for both C3a des Arg and C4a des Arg may be useful in assessing a number of pathological conditions. The use of potent protease inhibitors, such as Futhan, in conjunction with EDTA, may allow complement activation factors to be quantitated specifically via inhibition of non-specific protease formation of C3a des Arg.

Cross Reactivity

Compound Cross Reactivity
Human Complement C3a des Arg 100%
Human Complement C3 1.28%
Human Complement C4a des Arg 0.40%
Human Complement C4 0.31%
Human Complement C5 des Arg 0.10%
Human Complement C5 0.05%

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "High", "replicates": 0, "mean": "3.491 ng/mL", "standardDeviation": null, "coefficientOfVariability": "28.6" }, { "reproducibilityType": "Inter", "sample": "Low", "replicates": 0, "mean": "0.753 ng/mL", "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Inter", "sample": "Media", "replicates": 0, "mean": "1.529 ng/mL", "standardDeviation": null, "coefficientOfVariability": "16.9" }, { "reproducibilityType": "Intra", "sample": "High", "replicates": 16, "mean": "12.3 ng/mL", "standardDeviation": null, "coefficientOfVariability": "11.1" }, { "reproducibilityType": "Intra", "sample": "Low conc", "replicates": 16, "mean": "1.86 ng/mL", "standardDeviation": null, "coefficientOfVariability": "8.7" }, { "reproducibilityType": "Intra", "sample": "Medium conc", "replicates": 16, "mean": "3.4 ng/mL", "standardDeviation": null, "coefficientOfVariability": "9.8" } ]
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Recovery

[ { "sample": "Plasma", "range": null, "average": "= 94.8" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement C3a des Arg also known as the C3a desArg fragment is a bioactive derivative of the complement component C3a. This peptide is generated through the enzymatic removal of the carboxy-terminal arginine from C3a produced by the action of carboxypeptidase N. Its molecular mass is approximately 9 kDa. It is widely expressed in circulating blood particularly during the process of immune response activation. As a functional part of the complement system C3a des Arg plays a significant role in modulating immune responses and inflammation.
Biological function summary

The proteolytic removal of the terminal arginine from C3a results in C3a desArg which reduces its ability to activate C3a receptors. Despite this reduction in receptor activity C3a desArg retains anti-inflammatory properties and interacts with other components within the immune system. Unlike C3a it does not serve as a potent anaphylatoxin. It is not typically part of a larger molecular complex but acts independently to exert its effects on the immune system including modulation of leukocyte activity and interaction with distinct receptors compared to C3a.

Pathways

Complement C3a des Arg functions within the complement cascade a central component of the innate immune system. It plays roles in both the classical and alternative complement pathways influencing the immune response through modulation of inflammation. It associates with other proteins like C5a in specific regulatory pathways. The transformation from C3a to C3a des Arg can also influence interactions within the coagulation system where the futhan collection kit is utilized for monitoring such protein interactions during experimental procedures.

Complement C3a des Arg has associations with inflammatory conditions such as rheumatoid arthritis and systemic lupus erythematosus. Its role in modulating inflammation suggests a potential connection to these diseases where excessive immune activation occurs. The protein also shows interactions with C3 a related complement protein which plays an important role in autoimmune pathologies. Understanding these associations helps in the development of therapeutic strategies using tools like the C3a ELISA kit and other diagnostic assays.
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Product protocols

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Target data

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation.. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed : 10432298, PubMed : 15833747, PubMed : 16333141, PubMed : 19615750, PubMed : 2909530, PubMed : 8376604, PubMed : 9059512).
See full target information C3
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed39964754

2025

Complement activation at the interface between adipocytes and cancer cells drives tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Andres Valdivia,Ana Maria Isac,Horacio Cardenas,Guangyuan Zhao,Yaqi Zhang,Hao Huang,Jian-Jun Wei,Mauricio Cuello-Fredes,Sumie Kato,Fernán Gómez-Valenzuela,Francoise Gourronc,Aloysius Klingelhutz,Daniela Matei

Toxicology reports 14:101869 PubMed39811821

2025

Titanium nanostructure mitigating doxorubicin-induced testicular toxicity in rats via regulating major autophagy signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Rehab M Abdel-Megeed,Abdel-Hamid Z Abdel-Hamid,Mai O Kadry

ESC heart failure 10:492-501 PubMed36316820

2022

Acylation-stimulating protein and heart failure progression in arrhythmogenic right ventricular cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Ren,Liang Chen,Xiao Chen,Ningning Zhang,Xiaogang Sun,Jiangping Song

ACS nano 16:10566-10580 PubMed35822898

2022

PEGylated Polyester Nanoparticles Trigger Adverse Events in a Large Animal Model of Trauma and in Naı̈ve Animals: Understanding Cytokine and Cellular Correlations with These Events.

Applications

Unspecified application

Species

Unspecified reactive species

Nuzhat Maisha,Chhaya Kulkarni,Narendra Pandala,Rose Zilberberg,Leasha Schaub,Leslie Neidert,Jacob Glaser,Jeremy Cannon,Vandana Janeja,Erin B Lavik

Journal of the American Society of Nephrology : JASN 33:1742-1756 PubMed35777783

2022

Complement C3a and C3a Receptor Activation Mediates Podocyte Injuries in the Mechanism of Primary Membranous Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Gao,Zhao Cui,Ming-Hui Zhao

EJHaem 3:86-96 PubMed35846208

2022

Complement activation during cardiopulmonary bypass and association with clinical outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Rengina Kefalogianni,Farah Kamani,Mihaela Gaspar,T C Aw,Jackie Donovan,Mike Laffan,Matthew C Pickering,Deepa J Arachchillage

Haematologica 104:1661-1675 PubMed30679324

2019

All- retinoic acid protects mesenchymal stem cells from immune thrombocytopenia by regulating the complement-interleukin-1β loop.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolu Zhu,Yanan Wang,Qian Jiang,Hao Jiang,Jin Lu,Yazhe Wang,Yuan Kong,Yingjun Chang,Lanping Xu,Jun Peng,Ming Hou,Xiaojun Huang,Xiaohui Zhang

Bioconjugate chemistry 29:2436-2447 PubMed29965731

2018

Engineering Intravenously Administered Nanoparticles to Reduce Infusion Reaction and Stop Bleeding in a Large Animal Model of Trauma.

Applications

Unspecified application

Species

Unspecified reactive species

Chimdiya Onwukwe,Nuzhat Maisha,Mark Holland,Matt Varley,Rebecca Groynom,DaShawn Hickman,Nishant Uppal,Andrew Shoffstall,Jeffrey Ustin,Erin Lavik

Scientific reports 8:3118 PubMed29449604

2018

Intravenous synthetic platelet (SynthoPlate) nanoconstructs reduce bleeding and improve 'golden hour' survival in a porcine model of traumatic arterial hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

DaShawn A Hickman,Christa L Pawlowski,Andrew Shevitz,Norman F Luc,Ann Kim,Aditya Girish,Joyann Marks,Simi Ganjoo,Stephanie Huang,Edward Niedoba,Ujjal D S Sekhon,Michael Sun,Mitchell Dyer,Matthew D Neal,Vikram S Kashyap,Anirban Sen Gupta

Molecular and clinical oncology 8:315-319 PubMed29435296

2018

Anaphylatoxin C3a: A potential biomarker for esophageal cancer diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Zhang,Lingzhi Sun
View all publications
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