AB195461
Human Complement C3b ELISA Kit
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(7 Publications)
Human Complement C3b ELISA Kit is a sandwich ELISA designed to quantify Human Complement C3b with a sensitivity of 67 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Wide dynamic range - quantifies 0.3 - 1.1 ng/mL
View Alternative Names
CPAMD1, C3, Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
1 Images
- sELISA
Supplier Data
Sandwich ELISA - Human Complement C3b ELISA Kit (AB195461)
Representative standard curve using ab195461.
Reactivity data
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Product details
Human Complement C3b ELISA Kit ab195461 is a sandwich ELISA to measure Human Complement C3b in serum, plasma, cerebral spinal fluid (CSF), saliva, urine, milk with a sensitivity of 67 pg/ml.
How the assay works
A Complement C3b specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C3b specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C3b captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Complement C3b ELISA Kit ab195461 protocol summary
1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.
How the assay works
A Complement C3b specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C3b specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C3b captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Complement C3b ELISA Kit ab195461 protocol summary
1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.
Precision
[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.05" } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
C3 also known as complement C3 or anti C3b is a central protein in the complement system with an approximate molecular mass of 187 kDa. This protein exists in blood plasma as an inactive precursor and is produced mainly in the liver. C3 gets activated through cleavage into two fragments C3a and C3b. The functional fragment C3b covalently attaches to pathogen surfaces through a process called opsonization allowing it to 'tag' pathogens for destruction by immune cells.
Biological function summary
Complement C3b plays an integral role in innate immunity. C3b is a part of the complement cascade forming complexes such as C3bBb which acts as a convertase in the amplification of the complement response. Through opsonization C3b promotes the binding and uptake of pathogens by phagocytes therefore enhancing immune defense.
Pathways
C3b integrates into the alternative and classical complement pathways. In these pathways C3b binds with factors like B and D contributing to the formation of the C5 convertase and progressing to the membrane attack complex. These interactions involve proteins such as C5 and Factor B essential for the effective destruction of pathogens and the clearance of immune complexes.
Complement C3b shows associations with diseases involving improper immune regulation such as atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD). Mutations affecting C3b's interactions with regulatory proteins such as Factor H can lead to uncontrolled complement activation which contributes to the pathology of these diseases. Understanding these interactions provides insights into therapeutic targets for managing complement-related diseases.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation.. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed : 10432298, PubMed : 15833747, PubMed : 16333141, PubMed : 19615750, PubMed : 2909530, PubMed : 8376604, PubMed : 9059512).
Publications (7)
Recent publications for all applications. Explore the full list and refine your search
Journal of neuroinflammation 19:217 PubMed36064709
2022
Elevated CSF and plasma complement proteins in genetic frontotemporal dementia: results from the GENFI study.
Applications
Unspecified application
Species
Unspecified reactive species
Emma L van der Ende,Carolin Heller,Aitana Sogorb-Esteve,Imogen J Swift,David McFall,Georgia Peakman,Arabella Bouzigues,Jackie M Poos,Lize C Jiskoot,Jessica L Panman,Janne M Papma,Lieke H Meeter,Elise G P Dopper,Martina Bocchetta,Emily Todd,David Cash,Caroline Graff,Matthis Synofzik,Fermin Moreno,Elizabeth Finger,Raquel Sanchez-Valle,Rik Vandenberghe,Robert Laforce,Mario Masellis,Maria Carmela Tartaglia,James B Rowe,Chris Butler,Simon Ducharme,Alexander Gerhard,Adrian Danek,Johannes Levin,Yolande A L Pijnenburg,Markus Otto,Barbara Borroni,Fabrizio Tagliavini,Alexandre de Mendonça,Isabel Santana,Daniela Galimberti,Sandro Sorbi,Henrik Zetterberg,Eric Huang,John C van Swieten,Jonathan D Rohrer,Harro Seelaar
Brain : a journal of neurology 145:1805-1817 PubMed34633446
2021
A data-driven disease progression model of fluid biomarkers in genetic frontotemporal dementia.
Applications
Unspecified application
Species
Unspecified reactive species
Emma L van der Ende,Esther E Bron,Jackie M Poos,Lize C Jiskoot,Jessica L Panman,Janne M Papma,Lieke H Meeter,Elise G P Dopper,Carlo Wilke,Matthis Synofzik,Carolin Heller,Imogen J Swift,Aitana Sogorb-Esteve,Arabella Bouzigues,Barbara Borroni,Raquel Sanchez-Valle,Fermin Moreno,Caroline Graff,Robert Laforce,Daniela Galimberti,Mario Masellis,Maria Carmela Tartaglia,Elizabeth Finger,Rik Vandenberghe,James B Rowe,Alexandre de Mendonça,Fabrizio Tagliavini,Isabel Santana,Simon Ducharme,Christopher R Butler,Alexander Gerhard,Johannes Levin,Adrian Danek,Markus Otto,Yolande A L Pijnenburg,Sandro Sorbi,Henrik Zetterberg,Wiro J Niessen,Jonathan D Rohrer,Stefan Klein,John C van Swieten,Vikram Venkatraghavan,Harro Seelaar
Journal of clinical immunology 41:1607-1620 PubMed34232441
2021
Elevated Expression Levels of Lung Complement Anaphylatoxin, Neutrophil Chemoattractant Chemokine IL-8, and RANTES in MERS-CoV-Infected Patients: Predictive Biomarkers for Disease Severity and Mortality.
Applications
Unspecified application
Species
Unspecified reactive species
Maaweya E Hamed,Asif Naeem,Haitham Alkadi,Aref A Alamri,Ahmad S AlYami,Abdullah AlJuryyan,Wael Alturaiki,Mushira Enani,Samia T Al-Shouli,Abdullah M Assiri,Bandar Alosaimi
Frontiers in immunology 12:668725 PubMed34276659
2021
Complement Anaphylatoxins and Inflammatory Cytokines as Prognostic Markers for COVID-19 Severity and In-Hospital Mortality.
Applications
Unspecified application
Species
Unspecified reactive species
Bandar Alosaimi,Ayman Mubarak,Maaweya E Hamed,Abdullah Z Almutairi,Ahmed A Alrashed,Abdullah AlJuryyan,Mushira Enani,Faris Q Alenzi,Wael Alturaiki
Nature : PubMed32866962
2020
Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.
Applications
Unspecified application
Species
Unspecified reactive species
Jiasheng Zhang,Dmitry Velmeshev,Kei Hashimoto,Yu-Hsin Huang,Jeffrey W Hofmann,Xiaoyu Shi,Jiapei Chen,Andrew M Leidal,Julian G Dishart,Michelle K Cahill,Kevin W Kelley,Shane A Liddelow,William W Seeley,Bruce L Miller,Tobias C Walther,Robert V Farese,J Paul Taylor,Erik M Ullian,Bo Huang,Jayanta Debnath,Torsten Wittmann,Arnold R Kriegstein,Eric J Huang
European journal of immunology 47:1200-1210 PubMed28444759
2017
Dysregulated CD46 shedding interferes with Th1-contraction in systemic lupus erythematosus.
Applications
sELISA
Species
Unspecified reactive species
Ursula Ellinghaus,Andrea Cortini,Christopher L Pinder,Gaelle Le Friec,Claudia Kemper,Timothy J Vyse
Cell 165:921-35 PubMed27114033
2016
Progranulin Deficiency Promotes Circuit-Specific Synaptic Pruning by Microglia via Complement Activation.
Applications
sELISA
Species
Human
Hansen Lui,Jiasheng Zhang,Stefanie R Makinson,Michelle K Cahill,Kevin W Kelley,Hsin-Yi Huang,Yulei Shang,Michael C Oldham,Lauren Herl Martens,Fuying Gao,Giovanni Coppola,Steven A Sloan,Christine L Hsieh,Charles C Kim,Eileen H Bigio,Sandra Weintraub,Marek-Marsel Mesulam,Rosa Rademakers,Ian R Mackenzie,William W Seeley,Anna Karydas,Bruce L Miller,Barbara Borroni,Roberta Ghidoni,Robert V Farese,Jeanne T Paz,Ben A Barres,Eric J Huang
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