AB324923

Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit

SimpleStep
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Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Complement C4 Binding Protein Beta (C4BPB) with a sensitivity of 54.47pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

View Alternative Names

C4b-binding protein beta chain, C4BPB

6 Images

Sandwich ELISA - Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (AB324923)

sELISA

Lab

Sandwich ELISA - Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (AB324923)

Interpolated concentration of native Complement C4 Binding Protein Beta was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2.5% human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Sample Diluent SSW.

sELISA

Lab

Sandwich ELISA - Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (AB324923)

Example of human Complement C4 Binding Protein Beta standard curve in 1X Sample Diluent SSW. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (AB324923)

Interpolated concentration of native Complement C4 Binding Protein Beta was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human serum 5%, human plasma (citrate) 5%, and human plasma (heparin) 5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Sample Diluent SSW.

sELISA

Lab

Sandwich ELISA - Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (AB324923)

Example of human Complement C4 Binding Protein Beta standard curve in 96-well vs. 384-well plate in 1X Sample Diluent SSW. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (AB324923)

Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Complement C4 Binding Protein Beta concentration was determined to be 30.6 ng/mL with a range of 17.8 – 42.8 ng/mL.

sELISA

Lab

Sandwich ELISA - Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (AB324923)

Interpolated concentration of native Complement C4 Binding Protein Beta was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human plasma (EDTA) 2.5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Sample Diluent SSW.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 54.47 pg/mL

Range

117.188 - 7500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (ab324923) is a rapid single-wash sandwich 90-min ELISA to measure Human Complement C4 Binding Protein Beta (C4BPB) in Serum, Plasma – Citrate, Plasma – EDTA, Plasma – Heparin, Cell Culture Media. This SimpleStep sensitivity is 54.47 pg/ml.

How the assay works

Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (ab324923) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Complement C4 Binding Protein Beta (C4BPB) (ab324923) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit only requires 12.5µL sample.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.3" } ]

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Recovery

[ { "sample": "Serum", "range": "85 - 97 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "91 - 97 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "87 - 94 %", "average": "= 90" }, { "sample": "Heparin Plasma", "range": "81 - 88 %", "average": "= 84" }, { "sample": "Cell culture media", "range": "90 - 100 %", "average": "= 95" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S.

See full target information C4BPB

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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