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AB108825

Human Complement C4 ELISA Kit

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(3 Publications)

Human Complement C4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Complement C4 in Human in Biofluids samples.

View Alternative Names

CO4, CPAMD2, C4A, Complement C4-A, Acidic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2

1 Images
Sandwich ELISA - Human Complement C4 ELISA Kit (AB108825)
  • sELISA

Unknown

Sandwich ELISA - Human Complement C4 ELISA Kit (AB108825)

Representative Standard Curve using ab108825

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Milk, Saliva, Urine

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 35 pg/mL

Range

0.078 - 20 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Complement C4 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C4 levels in milk, urine, saliva, CSF, cell culture, cell lysate, and tissue samples.

A Complement C4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C4 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C4 captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement component 4 (C4) is a significant protein in the complement system also known as C4 complement. C4 has a molecular mass of approximately 206 kDa and mainly expresses in the liver. It is present in the blood plasma. C4 plays an important role in the immunological response and activates classical and lectin complement pathways. It serves a mechanical function by binding to pathogen surfaces and facilitating the cleavage that leads to the downstream activation of the complement cascade. There's a C4 ELISA test that allows for quantifying C4 levels in serum which is important for clinical diagnostics.
Biological function summary

Complement C4 participates in the immune defense by marking pathogens for destruction and enhancing phagocytosis. C4 is a part of a complex with other complement proteins like C3 and C1 to initiate the formation of the C3 convertase enzyme. This action amplifies the immune response leading to the clearance of microorganisms and apoptotic cells. C4 also plays a role in regulating the inflammation process maintaining homeostasis in the immune system.

Pathways

C4 fits into the complement activation pathway specifically the classical and lectin pathways. These pathways are important for innate immunity and connect with components like complement C3 and complement C1. Through the classical pathway C4 gets activated after binding to antibodies that have recognized antigens leading to a chain reaction that strengthens the immune response. The lectin pathway also requires C4 activation through different stimuli solidifying C4's central role in immune system signaling networks.

Abnormal C4 activity associates with autoimmune diseases like systemic lupus erythematosus (SLE) and rheumatoid arthritis. Low levels of complement C4 in serum can indicate these autoimmune conditions where the immune system mistakenly attacks its own tissues. C4 also connects to other proteins like complement component C1q in the context of SLE. C4 deficiencies or abnormalities may contribute to the development or exacerbation of these diseases highlighting its importance in disease pathogenesis and highlighting its value as a target for diagnostic tests such as C4 ELISA or serum complement tests.
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Product protocols

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Target data

Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
See full target information C4A

Additional targets

C4B
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34299197

2021

Complement C4 Is Reduced in iPSC-Derived Astrocytes of Autism Spectrum Disorder Subjects.

Applications

Unspecified application

Species

Unspecified reactive species

Fernanda Mansur,André Luiz Teles E Silva,Ana Karolyne Santos Gomes,Juliana Magdalon,Janaina Sena de Souza,Karina Griesi-Oliveira,Maria Rita Passos-Bueno,Andréa Laurato Sertié

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 46:1140-1144 PubMed32961544

2020

Complement component C4 levels in the cerebrospinal fluid and plasma of patients with schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Juan A Gallego,Emily A Blanco,Christopher Morell,Todd Lencz,Anil K Malhotra

Cell host & microbe 25:617-629.e7 PubMed30926239

2019

Complement C4 Prevents Viral Infection through Capsid Inactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Bottermann,Stian Foss,Sarah L Caddy,Dean Clift,Laurens M van Tienen,Marina Vaysburd,James Cruickshank,Kevin O'Connell,Jessica Clark,Keith Mayes,Katie Higginson,Heidrun E Lode,Martin B McAdam,Inger Sandlie,Jan Terje Andersen,Leo C James
View all publications
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