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AB125963

Human Complement C5 ELISA Kit

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(12 Publications)

Human Complement C5 ELISA Kit is a sandwich ELISA used to quantify Human Complement C5 with a sensitivity of 63 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Wide dynamic range – quantifies 0.156 – 10 ng/mL

View Alternative Names

CPAMD4, C5, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

2 Images
Sandwich ELISA - Human Complement C5 ELISA Kit (AB125963)
  • sELISA

Lab

Sandwich ELISA - Human Complement C5 ELISA Kit (AB125963)

Complement 5 measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).

Sandwich ELISA - Human Complement C5 ELISA Kit (AB125963)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement C5 ELISA Kit (AB125963)

Representative Standard Curve using ab125963

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Saliva, Plasma, Milk, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 63 pg/mL

Range

0.156 - 10 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

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Product details

Human Complement C5 ELISA Kit ab125963 is a sandwich ELISA kit to measure Human Complement C5 in Serum, Plasma, Cerebral Spinal Fluid, Saliva, and Milk with a sensitivity of 63 pg/mL.

How the assay works

Human Complement C5 ELISA Kit ab125963 uses a pair of antibodies each specific of different epitopes at the target of interest : capture antibody is coated on the surface of the multi-well plate to facilitate the immobilization of the antigen. The other antibody is conjugated and facilitates the detection of the antigen.

Assay Specificity

This kit recognizes both native human Complement C5 in serum, plasma, saliva, milk and CSF samples. No significant cross-reactivity observed with human complement C1,C2, C3, C4, C6, C7, C8, C9, factor B, factor D, factor H, factor I, and factor P proteins.

Human Complement C5 ELISA Kit ab125963 protocol summary

1. Add samples and standards to wells. Incubate at room temperature
2. Wash each well and add enzyme-antibody conjugate. Incubate at room temperature
3. Wash each well and add Streptavidin Solution. Incubate at room temperature
4. Add TMB Solution to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read immediately

How other researchers are using Human Complement C5 ELISA Kit ab125963

Human Complement C5 ELISA Kit ab125963 has been used to study RNAi therapeutics targeting C5 (1), and the pharmacokinetics of anti-C5 antibodies (2).

References:

(1) L. Kusner et al., 2019, PMID: 31193726
(2) A. Latuszek et al., 2020, PMID: 32384086

Get results in 90 minutes with our SimpleStep ELISA® kit

- Human Complement C5 ELISA Kit ab323604
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C5 also known as complement 5 is a protein with a mass of approximately 190 kDa. This protein plays a mechanical role as part of the complement system within the immune response. C5 is expressed in the bloodstream and is produced mainly by the liver. Upon activation it splits into C5a and C5b where C5a acts as a potent anaphylatoxin while C5b initiates the assembly of the membrane attack complex. Due to its important functions the production and regulation of C5 are tightly controlled within the body.
Biological function summary

The activation of this target contributes significantly to inflammatory processes and host defense. It is part of the larger complement system where it aligns with other complement proteins like C3 and forms a pathway important for immune system functioning. C5 is integral to the terminal complement complex formation which results in the lysis of target cells. The cleavage product C5a has near-instant effects as a chemotactic agent recruiting other immune cells to sites of infection.

Pathways

The protein has a fundamental position in the classical and alternative complement pathways. These pathways converge at the level of C5 where its cleavage is necessary for further downstream effects. C5 interacts extensively with C3 in these pathways highlighting their joint role in amplifying the immune response. The membrane attack complex which C5 helps initiate is a vital endpoint in these pathways promoting the destruction of pathogen cell membranes.

C5 has been linked to conditions like hereditary angioedema and atypical hemolytic uremic syndrome. Dysregulation of C5 or its pathways can lead to chronic inflammation excessive tissue damage or inefficient pathogen clearance. In such conditions related proteins like C3 also show altered activity patterns. Targeting C5 with specific antibodies has emerged as a therapeutic strategy to manage these diseases aiming to mitigate the pathological activation of the complement system.
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Product protocols

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Target data

Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 6554279). Activated downstream of classical, alternative, lectin and GZMK complement pathways (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 39914456, PubMed : 39814882, PubMed : 6554279).. Complement C5b. Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 26841837, PubMed : 27052168, PubMed : 30552328, PubMed : 30643019). Complement C5b is generated following cleavage by C5 convertase and initiates formation of the MAC complex : C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9 (PubMed : 30552328, PubMed : 30643019). During MAC complex assembly, the C5b6 subcomplex, composed of complement C5b and C6, associates with the outer leaflet of target cell membrane, reducing the energy for membrane bending (PubMed : 30552328, PubMed : 32569291).. C5a anaphylatoxin. Mediator of local inflammatory process released following cleavage by C5 convertase (PubMed : 8182049, PubMed : 9553099). Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed : 36806352, PubMed : 37852260, PubMed : 37169960, PubMed : 8182049, PubMed : 9553099). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation (PubMed : 342601, PubMed : 37852260, PubMed : 37169960, PubMed : 5765461, PubMed : 8182049, PubMed : 9553099).
See full target information C5
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed39964754

2025

Complement activation at the interface between adipocytes and cancer cells drives tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Andres Valdivia,Ana Maria Isac,Horacio Cardenas,Guangyuan Zhao,Yaqi Zhang,Hao Huang,Jian-Jun Wei,Mauricio Cuello-Fredes,Sumie Kato,Fernán Gómez-Valenzuela,Francoise Gourronc,Aloysius Klingelhutz,Daniela Matei

Biomarker insights 19:11772719241257739 PubMed38911905

2024

Mass Spectrometry Proteomics Characterization of Plasma Biomarkers for Colorectal Cancer Associated With Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Víctor Urbiola-Salvador,Agnieszka Jabłońska,Dominika Miroszewska,Weronika Kamysz,Katarzyna Duzowska,Kinga Drężek-Chyła,Ronny Baber,René Thieme,Ines Gockel,Marek Zdrenka,Ewa Śrutek,Łukasz Szylberg,Michał Jankowski,Dariusz Bała,Wojciech Zegarski,Tomasz Nowikiewicz,Wojciech Makarewicz,Agnieszka Adamczyk,Aleksandra Ambicka,Marcin Przewoźnik,Agnieszka Harazin-Lechowska,Janusz Ryś,Katarzyna Macur,Paulina Czaplewska,Natalia Filipowicz,Arkadiusz Piotrowski,Jan P Dumanski,Zhi Chen

Placenta 139:112-119 PubMed37356366

2023

Early pregnancy biomarker discovery study for spontaneous preterm birth.

Applications

Unspecified application

Species

Unspecified reactive species

Rik H J Beernink,Joost H N Schuitemaker,Eva F Zwertbroek,Sicco A Scherjon,Thomas I F H Cremers

BMC immunology 23:42 PubMed36088289

2022

The potential pathogenic roles of S100A8/A9 and S100A12 in patients with MPO-ANCA-positive vasculitis.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Bai,Peng-Cheng Xu,Tong Chen,Hao-Miao Zhang,Si-Jing Wu,Xia Yang,Shan Gao,Jun-Ya Jia,Jian-Qing Jiang,Tie-Kun Yan

Scientific reports 12:14079 PubMed35982097

2022

Efficient engraftment and viral transduction of human hepatocytes in an FRG rat liver humanization model.

Applications

Unspecified application

Species

Unspecified reactive species

Marisa Carbonaro,Jeffrey Lee,Evangelos Pefanis,Mathieu Desclaux,Kehui Wang,Alexander Pennington,Hui Huang,Alejo Mujica,Jose Rojas,Roxanne Ally,Daniel Kennedy,Michael Brown,Vitaliy Rogulin,Sven Moller-Tank,Leah Sabin,Brian Zambrowicz,Gavin Thurston,Zhe Li

PloS one 17:e0269749 PubMed35709087

2022

Pharmacokinetics and pharmacodynamics of pozelimab alone or in combination with cemdisiran in non-human primates.

Applications

Unspecified application

Species

Unspecified reactive species

Kishor Devalaraja-Narashimha,Cong Huang,Marc Cao,Ya Ping Chen,Anna Borodovsky,William C Olson,Lori G Morton,Marc W Retter

PloS one 15:e0231892 PubMed32384086

2020

Inhibition of complement pathway activation with Pozelimab, a fully human antibody to complement component C5.

Applications

Unspecified application

Species

Unspecified reactive species

Adrianna Latuszek,Yashu Liu,Olav Olsen,Randi Foster,Marc Cao,Irena Lovric,Ming Yuan,Nina Liu,Henry Chen,Qian Zhang,Hui Xiao,Carola Springer,George Ehrlich,Vishal Kamat,Ashique Rafique,Ying Hu,Pamela Krueger,Tammy Huang,William Poueymirou,Robert Babb,Michael P Rosconi,Marc W Retter,Gang Chen,Lori Morton,Brian Zambrowicz,Jingtai Cao,Carmelo Romano,William C Olson

Molecular therapy. Methods & clinical development 13:484-492 PubMed31193726

2019

Investigational RNAi Therapeutic Targeting C5 Is Efficacious in Pre-clinical Models of Myasthenia Gravis.

Applications

Unspecified application

Species

Unspecified reactive species

Linda L Kusner,Kristina Yucius,Manjistha Sengupta,Andrew G Sprague,Dhruv Desai,Tuyen Nguyen,Klaus Charisse,Satya Kuchimanchi,Rajeev Kallanthottathil,Kevin Fitzgerald,Henry J Kaminski,Anna Borodovsky

EBioMedicine 41:185-199 PubMed30773478

2019

DNA damage repair alterations modulate M2 polarization of microglia to remodel the tumor microenvironment via the p53-mediated MDK expression in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangqi Meng,Chunbin Duan,Hengyuan Pang,Qun Chen,Bo Han,Caijun Zha,Magafurov Dinislam,Pengfei Wu,Ziwei Li,Shihong Zhao,Ruijia Wang,Lin Lin,Chuanlu Jiang,Jinquan Cai

FEBS open bio 8:556-569 PubMed29632809

2018

Salivary proteome profiling of oral squamous cell carcinoma in a Hungarian population.

Applications

Unspecified application

Species

Unspecified reactive species

Éva Csősz,Bernadett Márkus,Zsuzsanna Darula,Katalin F Medzihradszky,Judit Nemes,Emese Szabó,József Tőzsér,Csongor Kiss,Ildikó Márton
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