Human Complement C5 ELISA Kit is a sandwich ELISA used to quantify Human Complement C5 with a sensitivity of 63 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Wide dynamic range – quantifies 0.156 – 10 ng/mL
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- BMC immunology 23:422022The potential pathogenic roles of S100A8/A9 and S100A12 in patients with MPO-ANCA-positive vasculitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue Bai,Peng-Cheng Xu,Tong Chen,Hao-Miao Zhang,Si-Jing Wu,Xia Yang,Shan Gao,Jun-Ya Jia,Jian-Qing Jiang,Tie-Kun YanPubMed 36088289
- Scientific reports 12:140792022Efficient engraftment and viral transduction of human hepatocytes in an FRG rat liver humanization model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarisa Carbonaro,Jeffrey Lee,Evangelos Pefanis,Mathieu Desclaux,Kehui Wang,Alexander Pennington,Hui Huang,Alejo Mujica,Jose Rojas,Roxanne Ally,Daniel Kennedy,Michael Brown,Vitaliy Rogulin,Sven Moller-Tank,Leah Sabin,Brian Zambrowicz,Gavin Thurston,Zhe LiPubMed 35982097
- PloS one 17:e02697492022Pharmacokinetics and pharmacodynamics of pozelimab alone or in combination with cemdisiran in non-human primates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKishor Devalaraja-Narashimha,Cong Huang,Marc Cao,Ya Ping Chen,Anna Borodovsky,William C Olson,Lori G Morton,Marc W RetterPubMed 35709087
- PloS one 15:e02318922020Inhibition of complement pathway activation with Pozelimab, a fully human antibody to complement component C5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrianna Latuszek et. al.PubMed 32384086
- Molecular therapy. Methods & clinical development 13:484-4922019Investigational RNAi Therapeutic Targeting C5 Is Efficacious in Pre-clinical Models of Myasthenia Gravis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinda L Kusner et. al.PubMed 31193726
- EBioMedicine 41:185-1992019DNA damage repair alterations modulate M2 polarization of microglia to remodel the tumor microenvironment via the p53-mediated MDK expression in glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangqi Meng et. al.PubMed 30773478
- FEBS open bio 8:556-5692018Salivary proteome profiling of oral squamous cell carcinoma in a Hungarian population.Applications:Unspecified applicationReactive species:Unspecified reactive speciesÉva Csősz,Bernadett Márkus,Zsuzsanna Darula,Katalin F Medzihradszky,Judit Nemes,Emese Szabó,József Tőzsér,Csongor Kiss,Ildikó MártonPubMed 29632809
- Stem cell research & therapy 8:1712017Minocycline modulates NFκB phosphorylation and enhances antimicrobial activity against Staphylococcus aureus in mesenchymal stromal/stem cells.
- Investigative ophthalmology & visual science 55:4776-892014Associations between abnormal rod-mediated dark adaptation and health and functioning in older adults with normal macular health.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Saliva, Plasma, Milk, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.156 - 10 ng/mL
- Assay time
- 4h
- Sensitivity
- = 63 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. C5a anaphylatoxin. Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed:8182049). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Alternative names
CPAMD4, C5, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
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Human Complement C5 ELISA Kit is a sandwich ELISA used to quantify Human Complement C5 with a sensitivity of 63 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Wide dynamic range – quantifies 0.156 – 10 ng/mL
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Saliva, Plasma, Milk, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.156 - 10 ng/mL
- Assay time
- 4h
- Assay Platform
- Microplate
- Sensitivity
- = 63 pg/mL
- General recovery
- 99%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 5.2 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 10.3 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Human Complement C5 ELISA Kit ab125963 is a sandwich ELISA kit to measure Human Complement C5 in Serum, Plasma, Cerebral Spinal Fluid, Saliva, and Milk with a sensitivity of 63 pg/mL.
How the assay works
Human Complement C5 ELISA Kit ab125963 uses a pair of antibodies each specific of different epitopes at the target of interest : capture antibody is coated on the surface of the multi-well plate to facilitate the immobilization of the antigen. The other antibody is conjugated and facilitates the detection of the antigen.
Assay Specificity
This kit recognizes both native human Complement C5 in serum, plasma, saliva, milk and CSF samples. No significant cross-reactivity observed with human complement C1,C2, C3, C4, C6, C7, C8, C9, factor B, factor D, factor H, factor I, and factor P proteins.
Human Complement C5 ELISA Kit ab125963 protocol summary
1. Add samples and standards to wells. Incubate at room temperature
2. Wash each well and add enzyme-antibody conjugate. Incubate at room temperature
3. Wash each well and add Streptavidin Solution. Incubate at room temperature
4. Add TMB Solution to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read immediately
How other researchers are using Human Complement C5 ELISA Kit ab125963
Human Complement C5 ELISA Kit ab125963 has been used to study RNAi therapeutics targeting C5 (1), and the pharmacokinetics of anti-C5 antibodies (2).
References:
(1) L. Kusner et al., 2019, PMID: 31193726
(2) A. Latuszek et al., 2020, PMID: 32384086
Get results in 90 minutes with our SimpleStep ELISA® kit
- Human Complement C5 ELISA Kit Human Complement C5 ELISA Kit ab323604
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C5 also known as complement 5 is a protein with a mass of approximately 190 kDa. This protein plays a mechanical role as part of the complement system within the immune response. C5 is expressed in the bloodstream and is produced mainly by the liver. Upon activation it splits into C5a and C5b where C5a acts as a potent anaphylatoxin while C5b initiates the assembly of the membrane attack complex. Due to its important functions the production and regulation of C5 are tightly controlled within the body.
- Biological function summary
The activation of this target contributes significantly to inflammatory processes and host defense. It is part of the larger complement system where it aligns with other complement proteins like C3 and forms a pathway important for immune system functioning. C5 is integral to the terminal complement complex formation which results in the lysis of target cells. The cleavage product C5a has near-instant effects as a chemotactic agent recruiting other immune cells to sites of infection.
- Pathways
The protein has a fundamental position in the classical and alternative complement pathways. These pathways converge at the level of C5 where its cleavage is necessary for further downstream effects. C5 interacts extensively with C3 in these pathways highlighting their joint role in amplifying the immune response. The membrane attack complex which C5 helps initiate is a vital endpoint in these pathways promoting the destruction of pathogen cell membranes.
- Associated diseases and disorders
C5 has been linked to conditions like hereditary angioedema and atypical hemolytic uremic syndrome. Dysregulation of C5 or its pathways can lead to chronic inflammation excessive tissue damage or inefficient pathogen clearance. In such conditions related proteins like C3 also show altered activity patterns. Targeting C5 with specific antibodies has emerged as a therapeutic strategy to manage these diseases aiming to mitigate the pathological activation of the complement system.
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2 product images
Sandwich ELISA - Human Complement C5 ELISA Kit (ab125963)
Complement 5 measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
Sandwich ELISA - Human Complement C5 ELISA Kit (ab125963)
Representative Standard Curve using ab125963
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