AB323604

Human Complement C5 ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Human Complement C5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Complement C5 with a sensitivity of 0.812 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CPAMD4, C5, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

6 Images

Sandwich ELISA - Human Complement C5 ELISA Kit (AB323604)

sELISA

Supplier Data

Sandwich ELISA - Human Complement C5 ELISA Kit (AB323604)

Example of human Complement C5 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Complement C5 ELISA Kit (AB323604)

Interpolated concentration of native Complement C5 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% HepG2 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Complement C5 ELISA Kit (AB323604)

Interpolated concentration of native Complement C5 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HepG2 cell culture supernatant 25%, and cerebrospinal fluid 50%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Complement C5 ELISA Kit (AB323604)

Example of human Complement C5 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Complement C5 ELISA Kit (AB323604)

Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Complement C5 concentration was determined to be 21.5 μg/mL with a range of 16.2 – 25.7 μg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Complement C5 ELISA Kit (AB323604)

Interpolated concentration of native Complement C5 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 100, plasma (citrate) 1 : 100, plasma (EDTA) 1 : 200, and plasma (heparin) 1 : 100. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.812 ng/mL

Range

2.5 - 160 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human Complement C5 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Complement C5 protein in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Cerebral Spinal Fluid. Quantitate Human Complement C5 with 0.812 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

style

left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.4" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "101 - 122 %", "average": "= 113" }, { "sample": "Citrate plasma", "range": "100 - 113 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "96 - 100 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "90 - 100 %", "average": "= 95" }, { "sample": "Cell culture supernatant", "range": "90 - 100 %", "average": "= 96" }, { "sample": "Cerebral Spinal Fluid", "range": "98 - 107 %", "average": "= 101" } ]

style

left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab323604-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB323604-CMP08" }, { "size":"20 x 1 Vial", "name":"Human Complement C5 Lyophilized Recombinant Protein", "number":"AB323604-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human Complement C5 Detector Antibody", "number":"AB323604-CMP02" }, { "size":"10 x 1 Vial", "name":"Lyophilized Human Complement C5 Capture Antibody", "number":"AB323604-CMP01" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323604-CMP05" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB323604-CMP10" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB323604-CMP04" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323604-CMP06" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB323604-CMP07" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323604-CMP09" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab323604-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB323604-CMP08" }, { "size":"2 x 1 Vial", "name":"Human Complement C5 Lyophilized Recombinant Protein", "number":"AB323604-CMP03" }, { "size":"1 x 600 µL", "name":"10X Human Complement C5 Detector Antibody", "number":"AB323604-CMP02" }, { "size":"1 x 1 Vial", "name":"Lyophilized Human Complement C5 Capture Antibody", "number":"AB323604-CMP01" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323604-CMP05" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB323604-CMP10" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB323604-CMP07" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB323604-CMP11" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323604-CMP06" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB323604-CMP04" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab323604-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Human Complement C5 Lyophilized Recombinant Protein", "number":"AB323604-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Complement C5 Detector Antibody", "number":"AB323604-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized Human Complement C5 Capture Antibody", "number":"AB323604-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB323604-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323604-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB323604-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323604-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB323604-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB323604-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323604-CMP09", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

style

left-column

Product protocols

style

left-column

Target data

Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 6554279). Activated downstream of classical, alternative, lectin and GZMK complement pathways (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 39914456, PubMed : 39814882, PubMed : 6554279).. Complement C5b. Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 26841837, PubMed : 27052168, PubMed : 30552328, PubMed : 30643019). Complement C5b is generated following cleavage by C5 convertase and initiates formation of the MAC complex : C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9 (PubMed : 30552328, PubMed : 30643019). During MAC complex assembly, the C5b6 subcomplex, composed of complement C5b and C6, associates with the outer leaflet of target cell membrane, reducing the energy for membrane bending (PubMed : 30552328, PubMed : 32569291).. C5a anaphylatoxin. Mediator of local inflammatory process released following cleavage by C5 convertase (PubMed : 8182049, PubMed : 9553099). Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed : 36806352, PubMed : 37852260, PubMed : 37169960, PubMed : 8182049, PubMed : 9553099). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation (PubMed : 342601, PubMed : 37852260, PubMed : 37169960, PubMed : 5765461, PubMed : 8182049, PubMed : 9553099).

See full target information C5

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com