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AB193695

Human Complement C5a ELISA Kit

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(9 Publications)

Human Complement C5a ELISA Kit is a sandwich ELISA designed to quantify Human Complement C5a with a sensitivity of 31 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 31 - 2000 pg/mL

View Alternative Names

CPAMD4, C5, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

2 Images
Sandwich ELISA - Human Complement C5a ELISA Kit (AB193695)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement C5a ELISA Kit (AB193695)

Standard Curve in Assay Diluent A using ab193695 Complement C5a Human ELISA Kit.

Sandwich ELISA - Human Complement C5a ELISA Kit (AB193695)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement C5a ELISA Kit (AB193695)

Standard Curve in Assay Diluent B using ab193695 Complement C5a Human ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 31 pg/mL

Range

31 - 2000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Complement C5a ELISA Kit ab193695 is a sandwich ELISA to measure Human Complement C5a in serum, plasma, cell culture supernatant with a sensitivity of 31 pg/ml.

How the assay works

This assay employs an antibody specific for Human C5a coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures C5a present in the samples. The wells are washed and biotinylated anti-Human C5a antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of C5a bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Complement C5a ELISA Kit ab193695 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "122 - 138 %", "average": "= 127.1" }, { "sample": "Plasma", "range": "114 - 130 %", "average": "= 122.5" }, { "sample": "Cell culture media", "range": "70 - 104 %", "average": "= 92.43" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193695-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193695-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB193695-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193695-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated Human C5a Microplate (12 Strips x 8 wells)", "number":"AB193695-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193695-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193695-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human C5a Standards (lyophilized)", "number":"AB193695-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB193695-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human C5a detection antibody", "number":"AB193695-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C5 also known as complement 5 is a protein with a mass of approximately 190 kDa. This protein plays a mechanical role as part of the complement system within the immune response. C5 is expressed in the bloodstream and is produced mainly by the liver. Upon activation it splits into C5a and C5b where C5a acts as a potent anaphylatoxin while C5b initiates the assembly of the membrane attack complex. Due to its important functions the production and regulation of C5 are tightly controlled within the body.
Biological function summary

The activation of this target contributes significantly to inflammatory processes and host defense. It is part of the larger complement system where it aligns with other complement proteins like C3 and forms a pathway important for immune system functioning. C5 is integral to the terminal complement complex formation which results in the lysis of target cells. The cleavage product C5a has near-instant effects as a chemotactic agent recruiting other immune cells to sites of infection.

Pathways

The protein has a fundamental position in the classical and alternative complement pathways. These pathways converge at the level of C5 where its cleavage is necessary for further downstream effects. C5 interacts extensively with C3 in these pathways highlighting their joint role in amplifying the immune response. The membrane attack complex which C5 helps initiate is a vital endpoint in these pathways promoting the destruction of pathogen cell membranes.

C5 has been linked to conditions like hereditary angioedema and atypical hemolytic uremic syndrome. Dysregulation of C5 or its pathways can lead to chronic inflammation excessive tissue damage or inefficient pathogen clearance. In such conditions related proteins like C3 also show altered activity patterns. Targeting C5 with specific antibodies has emerged as a therapeutic strategy to manage these diseases aiming to mitigate the pathological activation of the complement system.
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Product protocols

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Target data

Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 6554279). Activated downstream of classical, alternative, lectin and GZMK complement pathways (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 39914456, PubMed : 39814882, PubMed : 6554279).. Complement C5b. Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 26841837, PubMed : 27052168, PubMed : 30552328, PubMed : 30643019). Complement C5b is generated following cleavage by C5 convertase and initiates formation of the MAC complex : C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9 (PubMed : 30552328, PubMed : 30643019). During MAC complex assembly, the C5b6 subcomplex, composed of complement C5b and C6, associates with the outer leaflet of target cell membrane, reducing the energy for membrane bending (PubMed : 30552328, PubMed : 32569291).. C5a anaphylatoxin. Mediator of local inflammatory process released following cleavage by C5 convertase (PubMed : 8182049, PubMed : 9553099). Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed : 36806352, PubMed : 37852260, PubMed : 37169960, PubMed : 8182049, PubMed : 9553099). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation (PubMed : 342601, PubMed : 37852260, PubMed : 37169960, PubMed : 5765461, PubMed : 8182049, PubMed : 9553099).
See full target information C5
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed40338657

2025

Thrombospondin-1 inhibits alternative complement pathway activation in antineutrophil cytoplasmic antibody-associated vasculitis.

Applications

Unspecified application

Species

Unspecified reactive species

Swagata Konwar,Sophie Schroda,Manuel Rogg,Jessika Kleindienst,Eva L Decker,Martin Pohl,Barbara Zieger,Jens Panse,Hong Wang,Robert Grosse,Christoph Schell,Sabine Vidal,Xiaobo Liu,Christian Gorzelanny,Todor Tschongov,Karsten Häffner

Nature communications 15:10400 PubMed39613769

2024

Specific surface-modified iron oxide nanoparticles trigger complement-dependent innate and adaptive antileukaemia immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Li,Wen Wu,Qihui Liu,Qiong Wu,Ping Ren,Xi Xi,Haiyan Liu,Jiarui Zhao,Wei Zhang,Zizhun Wang,Yuanyuan Lv,Bin Tian,Shuang Sun,Jiaqi Cui,Yangyang Zhao,Jingyuan Wu,Mingyuan Gao,Fangfang Chen

Nature communications 15:10142 PubMed39587068

2024

Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mustafa Titiz,Lorenzo Landini,Daniel Souza Monteiro de Araujo,Matilde Marini,Viola Seravalli,Martina Chieca,Pasquale Pensieri,Marco Montini,Gaetano De Siena,Benedetta Pasquini,Silvia Vannuccini,Luigi Francesco Iannone,Thiago M Cunha,Giulia Brancolini,Elisa Bellantoni,Irene Scuffi,Alessandra Mastricci,Martina Tesi,Mariarosaria Di Tommaso,Felice Petraglia,Pierangelo Geppetti,Romina Nassini,Francesco De Logu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2302613 PubMed37345586

2023

FGF19-Induced Inflammatory CAF Promoted Neutrophil Extracellular Trap Formation in the Liver Metastasis of Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Li,Tianli Chen,Jialiang Liu,Yue Wang,Chunhuan Zhang,Lu Guo,Dandan Shi,Tingguo Zhang,Xishan Wang,Jie Li

Polymers 14: PubMed35890719

2022

Hemocompatibility Evaluation of Thai Silk Fibroin and Its Improvement with Low Molecular Weight Heparin Immobilization.

Applications

Unspecified application

Species

Unspecified reactive species

Tanrada Fungmongkonsatean,Jirapas Jongjitwimol,Pussadee Paensuwan,Teonchit Nuamchit,Duangduan Siriwittayawan,Sorada Kanokpanont,Siriporn Damrongsakkul,Piyanuch Thitiwuthikiat

EJHaem 3:86-96 PubMed35846208

2022

Complement activation during cardiopulmonary bypass and association with clinical outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Rengina Kefalogianni,Farah Kamani,Mihaela Gaspar,T C Aw,Jackie Donovan,Mike Laffan,Matthew C Pickering,Deepa J Arachchillage

Journal of clinical immunology 41:1607-1620 PubMed34232441

2021

Elevated Expression Levels of Lung Complement Anaphylatoxin, Neutrophil Chemoattractant Chemokine IL-8, and RANTES in MERS-CoV-Infected Patients: Predictive Biomarkers for Disease Severity and Mortality.

Applications

Unspecified application

Species

Unspecified reactive species

Maaweya E Hamed,Asif Naeem,Haitham Alkadi,Aref A Alamri,Ahmad S AlYami,Abdullah AlJuryyan,Wael Alturaiki,Mushira Enani,Samia T Al-Shouli,Abdullah M Assiri,Bandar Alosaimi

Frontiers in immunology 12:668725 PubMed34276659

2021

Complement Anaphylatoxins and Inflammatory Cytokines as Prognostic Markers for COVID-19 Severity and In-Hospital Mortality.

Applications

Unspecified application

Species

Unspecified reactive species

Bandar Alosaimi,Ayman Mubarak,Maaweya E Hamed,Abdullah Z Almutairi,Ahmed A Alrashed,Abdullah AlJuryyan,Mushira Enani,Faris Q Alenzi,Wael Alturaiki

Journal of cellular biochemistry 121:111-124 PubMed31190349

2019

MicroRNA-194 reduces inflammatory response and human dermal microvascular endothelial cells permeability through suppression of TGF-β/SMAD pathway by inhibiting THBS1 in chronic idiopathic urticaria.

Applications

Unspecified application

Species

Unspecified reactive species

Shengming Qu,Lei Yang,Zhe Liu
View all publications
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