Human Complement C5a ELISA Kit is a sandwich ELISA designed to quantify Human Complement C5a with a sensitivity of 31 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 31 - 2000 pg/mL
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- Polymers 14:2022Hemocompatibility Evaluation of Thai Silk Fibroin and Its Improvement with Low Molecular Weight Heparin Immobilization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTanrada Fungmongkonsatean,Jirapas Jongjitwimol,Pussadee Paensuwan,Teonchit Nuamchit,Duangduan Siriwittayawan,Sorada Kanokpanont,Siriporn Damrongsakkul,Piyanuch ThitiwuthikiatPubMed 35890719
- EJHaem 3:86-962022Complement activation during cardiopulmonary bypass and association with clinical outcomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRengina Kefalogianni,Farah Kamani,Mihaela Gaspar,T C Aw,Jackie Donovan,Mike Laffan,Matthew C Pickering,Deepa J ArachchillagePubMed 35846208
- Journal of clinical immunology 41:1607-16202021Elevated Expression Levels of Lung Complement Anaphylatoxin, Neutrophil Chemoattractant Chemokine IL-8, and RANTES in MERS-CoV-Infected Patients: Predictive Biomarkers for Disease Severity and Mortality.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaaweya E Hamed,Asif Naeem,Haitham Alkadi,Aref A Alamri,Ahmad S AlYami,Abdullah AlJuryyan,Wael Alturaiki,Mushira Enani,Samia T Al-Shouli,Abdullah M Assiri,Bandar AlosaimiPubMed 34232441
- Frontiers in immunology 12:6687252021Complement Anaphylatoxins and Inflammatory Cytokines as Prognostic Markers for COVID-19 Severity and In-Hospital Mortality.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBandar Alosaimi,Ayman Mubarak,Maaweya E Hamed,Abdullah Z Almutairi,Ahmed A Alrashed,Abdullah AlJuryyan,Mushira Enani,Faris Q Alenzi,Wael AlturaikiPubMed 34276659
- Journal of cellular biochemistry 121:111-1242019MicroRNA-194 reduces inflammatory response and human dermal microvascular endothelial cells permeability through suppression of TGF-β/SMAD pathway by inhibiting THBS1 in chronic idiopathic urticaria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengming Qu,Lei Yang,Zhe LiuPubMed 31190349
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31 - 2000 pg/mL
- Sensitivity
- = 31 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. C5a anaphylatoxin. Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed:8182049). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Alternative names
CPAMD4, C5, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
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Recommended products
Human Complement C5a ELISA Kit is a sandwich ELISA designed to quantify Human Complement C5a with a sensitivity of 31 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 31 - 2000 pg/mL
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31 - 2000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 31 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 10 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 127.1 | Range 122 - 138 % |
Sample type Plasma | Average % = 122.5 | Range 114 - 130 % |
Sample type Cell culture media | Average % = 92.43 | Range 70 - 104 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Complement C5a Human ELISA Kit (ab193695) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human C5A in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human C5a coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures C5a present in the samples. The wells are washed and biotinylated anti-Human C5a antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of C5a bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C5 also known as complement 5 is a protein with a mass of approximately 190 kDa. This protein plays a mechanical role as part of the complement system within the immune response. C5 is expressed in the bloodstream and is produced mainly by the liver. Upon activation it splits into C5a and C5b where C5a acts as a potent anaphylatoxin while C5b initiates the assembly of the membrane attack complex. Due to its important functions the production and regulation of C5 are tightly controlled within the body.
- Biological function summary
The activation of this target contributes significantly to inflammatory processes and host defense. It is part of the larger complement system where it aligns with other complement proteins like C3 and forms a pathway important for immune system functioning. C5 is integral to the terminal complement complex formation which results in the lysis of target cells. The cleavage product C5a has near-instant effects as a chemotactic agent recruiting other immune cells to sites of infection.
- Pathways
The protein has a fundamental position in the classical and alternative complement pathways. These pathways converge at the level of C5 where its cleavage is necessary for further downstream effects. C5 interacts extensively with C3 in these pathways highlighting their joint role in amplifying the immune response. The membrane attack complex which C5 helps initiate is a vital endpoint in these pathways promoting the destruction of pathogen cell membranes.
- Associated diseases and disorders
C5 has been linked to conditions like hereditary angioedema and atypical hemolytic uremic syndrome. Dysregulation of C5 or its pathways can lead to chronic inflammation excessive tissue damage or inefficient pathogen clearance. In such conditions related proteins like C3 also show altered activity patterns. Targeting C5 with specific antibodies has emerged as a therapeutic strategy to manage these diseases aiming to mitigate the pathological activation of the complement system.
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2 product images
Sandwich ELISA - Human Complement C5a ELISA Kit (ab193695)
Standard Curve in Assay Diluent A using ab193695 Complement C5a Human ELISA Kit.
Sandwich ELISA - Human Complement C5a ELISA Kit (ab193695)
Standard Curve in Assay Diluent B using ab193695 Complement C5a Human ELISA Kit.
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