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AB125965

Human Complement C6 ELISA Kit

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Human Complement C6 ELISA Kit is a Sandwich ELISA for the measurement of Human Complement C6 in Human in Biofluids samples.

View Alternative Names

Complement component C6, C6

3 Images
ELISA - Human Complement C6 ELISA Kit (AB125965)
  • ELISA

Lab

ELISA - Human Complement C6 ELISA Kit (AB125965)

Colorimetric standard curve : mean of duplicates (+/-) with background readings subtracted.

ELISA - Human Complement C6 ELISA Kit (AB125965)
  • ELISA

Lab

ELISA - Human Complement C6 ELISA Kit (AB125965)

Complement C6 measured in various biofluids showing quantity (microgram) per mL of sample tested.

Sandwich ELISA - Human Complement C6 ELISA Kit (AB125965)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement C6 ELISA Kit (AB125965)

Representative Standard Curve using ab125965.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Saliva, Urine, Plasma, Milk, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.14 ng/mL

Range

0.391 - 25 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Complement C6 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C6 in Human plasma, serum, saliva, urine, milk, and cell culture supernatants.

A Complement C6 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C6 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C6 captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab125965-1x96Tests", "assetComponentDetails": [ { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB125965-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB125965-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB125965-CMP08", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB125965-CMP03", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB125965-CMP06", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB125965-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Complement C6 Microplate (12 x 8 well strips)", "number":"AB125965-CMP04", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Complement C6 Antibody", "number":"AB125965-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Complement C6 Standard", "number":"AB125965-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement component C6 often called simply C6 is an essential protein in the complement system. It is part of the terminal complement pathway which leads to the formation of the membrane attack complex (MAC). C6 has a molecular weight of about 93 kDa and is mainly produced in the liver. It circulates in plasma and interacts with other complement proteins to form MAC aiding in pathogen elimination and cell lysis.
Biological function summary

The role of C6 is the progression and stabilization of the MAC complex which is part of the innate immune system's defense. C6 collaborates with other complement proteins such as C5b C7 C8 and C9. This cooperation helps penetrate the membrane of pathogenic cells leading to cell destruction. Detecting and quantifying C6 levels is possible using techniques like complement C6 ELISA and C6 graphs valuable for research into immune response mechanisms.

Pathways

The complement component C6 is an important player in the terminal complement pathway and the larger complement activation pathway. Within these pathways C6 interacts closely with proteins like C5b and C9 to facilitate the assembly of the MAC. C6's involvement is critical for mounting an efficient immune response against invading pathogens making it an important focus for immunology studies involving the complement cascade.

C6 deficiencies are linked to increased susceptibility to infections particularly by Neisseria species which cause diseases like meningitis. Individuals with C6-related complement deficiencies might show recurrent bacterial infections highlighting its role in immune defense. The protein's interplay with other complement components notably C5 and C7 can influence the disease states and inform potential therapeutic approaches aimed at boosting host defense mechanisms.
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Product protocols

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Target data

Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 22267737, PubMed : 22832194, PubMed : 26841837, PubMed : 27052168, PubMed : 30552328). The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways (PubMed : 30552328, PubMed : 39914456, PubMed : 39814882). The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 30552328). Together with component C5b, involved in MAC complex assembly : complement C5b and C6 associate with the outer leaflet of target cell membrane, reducing the energy for membrane bending (PubMed : 30552328, PubMed : 32569291).
See full target information C6
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Product promise

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