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AB230934

Human Complement C6 ELISA Kit

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(1 Publication)

Human Complement C6 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Complement C6 with a sensitivity of 71 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Complement component C6, C6

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Sandwich ELISA - Human Complement C6 ELISA Kit (AB230934)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement C6 ELISA Kit (AB230934)

Interpolated concentrations of native Complement C6 in Human serum, plasma and saliva samples.

The concentrations of Complement C6 were measured in duplicates, interpolated from the Complement C6 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 2000, plasma (citrate) 1 : 2000, plasma (EDTA) 1 : 2000, plasma (heparin) 1 : 2000, and saliva 1 : 2. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C6 concentration, corrected for dilution, was determined to be 21 μg/mL in serum, 19 μg/mL in plasma (citrate), 16 μg/mL in plasma (EDTA), 15 μg/mL in plasma (heparin), and 4 ng/mL (0.004 μg/mL) in saliva.

Sandwich ELISA - Human Complement C6 ELISA Kit (AB230934)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement C6 ELISA Kit (AB230934)

Serum from ten individual healthy Human male donors was measured in duplicate.

Interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean Complement C6 concentration was determined to be 23 μg/mL with a range of 16 – 29 μg/mL.

Sandwich ELISA - Human Complement C6 ELISA Kit (AB230934)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement C6 ELISA Kit (AB230934)

Example of Human Complement C6 standard curve in Sample Diluent NS + Enhancer.

The Complement C6 standard curve was prepared as described in Section 10.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 71 pg/mL

Range

0.2 - 15 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Complement C6 ELISA Kit (ab230934) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Complement C6 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, saliva, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Complement C6 with 71 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "77 - 92 %", "average": "= 84" }, { "sample": "Serum", "range": "94 - 108 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "83 - 105 %", "average": "= 95" }, { "sample": "Saliva", "range": "79 - 102 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "100 - 117 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "78 - 106 %", "average": "= 95" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab230934-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB230934-CMP07", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB230934-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Complement C6 Lyophilized Recombinant Protein", "number":"AB230934-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB230934-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Complement C6 Capture Antibody", "number":"AB230934-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB230934-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB230934-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB230934-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Complement C6 Detector Antibody", "number":"AB230934-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB230934-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB230934-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement component C6 often called simply C6 is an essential protein in the complement system. It is part of the terminal complement pathway which leads to the formation of the membrane attack complex (MAC). C6 has a molecular weight of about 93 kDa and is mainly produced in the liver. It circulates in plasma and interacts with other complement proteins to form MAC aiding in pathogen elimination and cell lysis.
Biological function summary

The role of C6 is the progression and stabilization of the MAC complex which is part of the innate immune system's defense. C6 collaborates with other complement proteins such as C5b C7 C8 and C9. This cooperation helps penetrate the membrane of pathogenic cells leading to cell destruction. Detecting and quantifying C6 levels is possible using techniques like complement C6 ELISA and C6 graphs valuable for research into immune response mechanisms.

Pathways

The complement component C6 is an important player in the terminal complement pathway and the larger complement activation pathway. Within these pathways C6 interacts closely with proteins like C5b and C9 to facilitate the assembly of the MAC. C6's involvement is critical for mounting an efficient immune response against invading pathogens making it an important focus for immunology studies involving the complement cascade.

C6 deficiencies are linked to increased susceptibility to infections particularly by Neisseria species which cause diseases like meningitis. Individuals with C6-related complement deficiencies might show recurrent bacterial infections highlighting its role in immune defense. The protein's interplay with other complement components notably C5 and C7 can influence the disease states and inform potential therapeutic approaches aimed at boosting host defense mechanisms.
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Product protocols

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Target data

Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 22267737, PubMed : 22832194, PubMed : 26841837, PubMed : 27052168, PubMed : 30552328). The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways (PubMed : 30552328, PubMed : 39914456, PubMed : 39814882). The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 30552328). Together with component C5b, involved in MAC complex assembly : complement C5b and C6 associate with the outer leaflet of target cell membrane, reducing the energy for membrane bending (PubMed : 30552328, PubMed : 32569291).
See full target information C6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 8: PubMed37227781

2023

Eculizumab treatment alters the proteometabolome beyond the inhibition of complement.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Nelke,Christina B Schroeter,Frauke Stascheit,Niklas Huntemann,Marc Pawlitzki,Alice Willison,Saskia Räuber,Nico Melzer,Ute Distler,Stefan Tenzer,Kai Stühler,Andreas Roos,Andreas Meisel,Sven G Meuth,Tobias Ruck
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Product promise

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For full details, please see our Terms & Conditions

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