Human Complement C7 ELISA Kit
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(3 Publications)
Human Complement C7 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Complement C7 in Human in Biofluids samples.
View Alternative Names
Complement component C7, C7
- sELISA
Supplier Data
Sandwich ELISA - Human Complement C7 ELISA Kit (AB125964)
Representative Standard Curve using ab125964.
Reactivity data
Product details
Abcam's Complement C7 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C7 in Human plasma, serum, urine, milk, saliva, and CSF samples.
A Complement C7 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C7 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C7 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
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Supplementary information
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Biological function summary
C7 functions as an important facilitator in the innate immune response being an integral part of the membrane attack complex (MAC). This complex forms transmembrane channels in the cell membrane of pathogens leading to their lysis and destruction. Through its interaction with complement components like C5b and C6 C7 enables the insertion and stabilization of the MAC. This process is important for the immune system’s ability to eliminate pathogens quickly and effectively protecting the host from microbial invaders.
Pathways
The complement system pathway is the primary biological pathway involving C7. Within this pathway C7 interacts closely with complement proteins C5 and C6 contributing to the terminal pathway that leads to pathogen lysis. Besides it is linked with the classical and alternative complement pathways both of which converge at the activation of C5. Through these interactions and processes C7 works synergistically with other complement proteins to execute robust immune defense mechanisms.
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Publications (3)
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JCI insight 8: PubMed37227781
2023
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BioMed research international 2020:8879758 PubMed33381596
2020
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Oncotarget 9:14862-14881 PubMed29599912
2018
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