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AB137971

Human Complement C8 ELISA Kit

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Human Complement C8 ELISA Kit is a Sandwich ELISA for the measurement of Human Complement C8 in Human in Biofluids samples.

View Alternative Names

Complement component C8 alpha chain, Complement component 8 subunit alpha, C8A

1 Images
Sandwich ELISA - Human Complement C8 ELISA Kit (AB137971)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement C8 ELISA Kit (AB137971)

Representative Standard Curve using ab137971

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Saliva, Milk, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

>= 0.24 ng/mL

Range

49 - 106 µg/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Complement C8 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C8 in Human plasma, serum, saliva, milk, and CSF samples.

A Complement C8 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C8 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C8 captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab137971-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"10X Diluent M Concentrate", "number":"AB137971-CMP01", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB137971-CMP07", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB137971-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB137971-CMP08", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB137971-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Complement C8 Antibody", "number":"AB137971-CMP10", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Complement C8 Standard", "number":"AB137971-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Complement C8 Microplate (12 x 8 well strips)", "number":"AB137971-CMP02", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB137971-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Complement C8 protein plays an important role in the immune response specifically as a component of the membrane attack complex (MAC). Known also simply as C8 it is composed of three subunits: C8α C8β and C8γ with a combined molecular mass of approximately 151 kDa. C8 is synthesized primarily by liver hepatocytes but its expression occurs in several other tissues such as monocytes and macrophages highlighting its significance in immune surveillance.
Biological function summary

The membrane attack complex is a pivotal element of the complement system where Complement C8 serves as a final assembly participant. It collaborates with other proteins such as C5b C6 and C7 to penetrate and lyse target cell membranes protecting the host against pathogenic invaders. C8 forms part of a larger complement cascade which activates sequentially ensuring swift response to microbial threats. This process highlights its relevance in innate immunity.

Pathways

Several significant biological processes involve Complement C8. The complement system and the coagulation pathway both rely on this protein's function. Within these pathways C8 associates closely with proteins such as C9 in forming the lytic pore of the MAC and C3b which facilitates the opsonization and clearance of pathogens. These collaborative actions support efficient immune defense and maintain homeostatic balance within the bloodstream.

Complement system dysregulation can link to immune complex diseases such as systemic lupus erythematosus (SLE) and paroxysmal nocturnal hemoglobinuria (PNH). In SLE improper complement activation possibly involving Complement C8 may lead to the formation of immune complexes that cause inflammation and tissue damage. In PNH the lack of complement inhibitory proteins results in red blood cell destruction where the unregulated MAC formation involving C8 plays a part in the hemolytic process. Understanding these connections helps unravel the complex roles that Complement C8 has in disease pathogenesis.
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Product protocols

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Target data

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself.
See full target information C8A
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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