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AB137972

Human Complement C9 ELISA Kit

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(3 Publications)

Human Complement C9 ELISA Kit is a sandwich ELISA designed to quantify Human Complement C9 with a sensitivity of 52 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Wide dynamic range - quantifies 0.234 - 15 ng/mL

View Alternative Names

Complement component C9, C9

1 Images
Sandwich ELISA - Human Complement C9 ELISA Kit (AB137972)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement C9 ELISA Kit (AB137972)

Representative Standard Curve using ab137972

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Saliva, Urine, Plasma, Milk, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 52 pg/mL

Range

0.234 - 15 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

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Product details

Human Complement C9 ELISA Kit ab137972 is a sandwich ELISA to measure Human Complement C9 in serum, plasma, cerebral spinal fluid (CSF), saliva, urine, milk with a sensitivity of 52 pg/ml.

How the assay works

A Complement C9 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C9 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C9 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Complement C9 ELISA Kit ab137972 protocol summary

1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.
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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.4" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C9 also known as complement component 9 is a protein that plays an essential mechanical role in the complement system. It typically associates in the formation of the membrane attack complex (MAC). The C9 protein weighing around 71 kDa expresses in the liver and is secreted into the blood plasma. Alternative names like 'Cloud 9 capture' and 'C9 tag' often refer to research tools or motifs derived from the C9 protein rather than the protein itself. Researchers frequently study C9 in mouse models where its physiological roles can be explored.
Biological function summary

C9 participates in defending against pathogens. It joins other complement components like C5b C6 C7 and C8 to form the MAC complex which facilitates cell lysis by forming pores in the membrane of target cells. This action directly results in microbial cell destruction serving a critical immune function. C9 is not only part of the MAC complex but also holds significance due to its unique function as the last step in the formation of the pore influencing the efficacy of pathogen elimination.

Pathways

C9 functions primarily within the complement cascade which includes the classical and alternative pathways. Its role is in the terminal pathway segment that ultimately leads to the formation of the MAC and follows the activation of C5 which cleaves to produce C5b. This connection extends to proteins like C3 which is upstream in the cascade and significantly impacts the cascade's activation highlighting C9's function as an integral terminal component in these pathways.

Mutations or deficits in C9 relate to an increased susceptibility to infections particularly those caused by Neisseria species. This association results directly from its important role in the MAC formation. Additionally deficiency of C9 links to autoimmune disorders where the immune response may be improperly regulated due to compromised MAC formation. Other complement components like C6 and C8 may also relate to these diseases due to their collaboration with C9 in forming the MAC illustrating the interconnectedness of these proteins within the immune response framework.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells (PubMed : 26841934, PubMed : 9212048, PubMed : 9634479). C9 is the pore-forming subunit of the MAC (PubMed : 26841934, PubMed : 30111885, PubMed : 4055801).
See full target information C9
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Proteomics. Clinical applications 14:e1900001 PubMed31715074

2019

Label-Free Quantitative Proteomics Identifies Novel Biomarkers for Distinguishing Tuberculosis Pleural Effusion from Malignant Pleural Effusion.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Pan,Xia Zhang,Hongyan Jia,Mailing Huang,Fei Liu,Jinghui Wang,Boping Du,Rongrong Wei,Qi Sun,Aiying Xing,Qi Li,Zongde Zhang

Experimental and therapeutic medicine 18:1417-1425 PubMed31316628

2019

Effect of haptoglobin on the treatment of chronic hepatitis B with interferon.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Ling Lv,Yi-Xuan Yang,Tong-Dong Shi

International journal of oncology 47:543-54 PubMed26058824

2015

C-reactive protein is a biomarker of AFP-negative HBV-related hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Sha She,Yi Xiang,Min Yang,Xiangchun Ding,Xiaoyan Liu,Lina Ma,Qing Liu,Bin Liu,Zhenhui Lu,Shiying Li,Yi Liu,Xiaoping Ran,Xiaoming Xu,Huaidong Hu,Peng Hu,Dazhi Zhang,Hong Ren,Yixuan Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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