JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB315062

Human Complement Factor B (Ba fragment) ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Complement Factor B (Ba fragment) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Complement Factor B (Ba fragment) with a sensitivity of 23.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

BF, BFD, CFB, Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, PBF2, Properdin factor B, GBG

4 Images
Sandwich ELISA - Human Complement Factor B (Ba fragment) ELISA Kit (AB315062)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement Factor B (Ba fragment) ELISA Kit (AB315062)

Example of Human Complement Factor B (Ba Fragment) standard curve in 1X Wash Buffer PT. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Complement Factor B (Ba fragment) ELISA Kit (AB315062)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement Factor B (Ba fragment) ELISA Kit (AB315062)

Interpolated concentration of native Complement Factor B (Ba Fragment) was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 2000, and plasma (heparin) 1 : 500. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

Sandwich ELISA - Human Complement Factor B (Ba fragment) ELISA Kit (AB315062)
  • sELISA

Lab

Sandwich ELISA - Human Complement Factor B (Ba fragment) ELISA Kit (AB315062)

Interpolated concentration of native Complement Factor B (Ba Fragment) was measured in duplicate. Undiluted samples are as follows : HepG2 cell culture supernatant 1 : 10. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

Sandwich ELISA - Human Complement Factor B (Ba fragment) ELISA Kit (AB315062)
  • sELISA

Lab

Sandwich ELISA - Human Complement Factor B (Ba fragment) ELISA Kit (AB315062)

Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Complement Factor B (Ba Fragment) concentration was determined to be 4041 ng/mL with a range of 2023 – 6080 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Serum, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 23.3 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB108823

Human Complement C3 ELISA Kit

Sandwich ELISA - Human Complement C3 ELISA Kit (AB108823)

  • 5
  • 2
Assay Kits

AB125963

Human Complement C5 ELISA Kit

Sandwich ELISA - Human Complement C5 ELISA Kit (AB125963)

  • 0
  • 0
Assay Kits

AB315320

Human C4a ELISA Kit

SimpleStep

Sandwich ELISA - Human C4a ELISA Kit (AB315320)

  • 0
  • 0
Assay Kits

AB252359

Human Complement factor H ELISA Kit

Recombinant|SimpleStep

ELISA - Human Complement factor H ELISA Kit (AB252359)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Complement Factor B (Ba fragment) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Complement Factor B (Ba fragment) protein in Serum, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human Complement Factor B (Ba fragment) with 23.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": "6.24 %", "standardDeviation": null, "coefficientOfVariability": null }, { "reproducibilityType": "Inter", "sample": "Bovine Serum Albumin", "replicates": 3, "mean": "3.94 %", "standardDeviation": null, "coefficientOfVariability": null } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "98 - 105 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "88 - 97 %", "average": "= 108" }, { "sample": "Cell culture supernatant", "range": "109 - 113 %", "average": "= 116" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315062-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Lyophilized Human Complement Factor B (Ba fragment) Capture Antibody", "number":"AB315062-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Complement Factor B (Ba fragment) Detector Antibody", "number":"AB315062-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Complement Factor B (Ba fragment) Lyophilized Recombinant Protein", "number":"AB315062-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB315062-CMP04", "productcode":"" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315062-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315062-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315062-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315062-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315062-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB315062-CMP10", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement Factor B also known as factor B complement is an important protein in the immune system. It weighs about 93 kDa and mainly expresses in the liver though it also circulates in the blood. This protein plays an important role in the alternative pathway of complement activation. Upon activation factor B combines with complement C3b to form the C3bB complex which then turns into the C3 convertase. This converts more C3 into C3b amplifying the immune response.
Biological function summary

Complement Factor B serves as a serine protease that is essential in the body's defense mechanisms. It is an integral part of the C3/C5 convertase enzyme complex. When C3b binds to factor B complement it splits into Ba and Bb fragments. The Bb fragment becomes part of the C3 convertase structure on microbial surfaces facilitating greater immune defense. Factor B's action allows the body to rapidly respond to pathogens contributing to innate immunity by helping opsonize and clear pathogens.

Pathways

This protein participates chiefly in the alternative complement pathway. It interacts closely with other complement components such as factor C3 and factor D to promote this pathway. The cascade initiated by complement factor B targets pathogens for destruction and assists in inflammation processes. It ensures a quick response in eliminating infectious agents before the adaptive immune system gets activated linking the innate and adaptive immune responses.

Research links Complement Factor B to conditions like age-related macular degeneration (AMD) and atypical hemolytic uremic syndrome (aHUS). In these disorders abnormal regulation or mutations in factor B can lead to excessive complement activation. For AMD this hyperactivity results in damage to retinal cells whereas in aHUS it causes issues in blood vessels and kidneys. Decay-accelerating factor and factor H also play notable roles here regulating this pathway to prevent overactivation that could harm healthy tissues.
style
left-column

Product protocols

style
left-column

Target data

Precursor of the catalytic component of the C3 and C5 convertase complexes of the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). The alternative complement pathway acts as an amplification loop that enhances other complement pathways (classical, lectin and GZMK) by promoting formation of additional C3 and C5 convertases (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). CFB is cleaved and activated by CFD to generate Ba and Bb chains; Bb chain constituting the catalytic component of the C3 and C5 convertases (PubMed : 6769474, PubMed : 9748277).. Complement factor B Bb. Serine protease component of the complement C3 and C5 convertase complexes of the alternative complement pathway (PubMed : 30643019, PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543). Following cleavage and activation by factor D (CFD), forms the C3 convertase together with complement C3b (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). As part of the C3 convertase, cleaves and activates C3 into C3a anaphylatoxin and C3b opsonin, the next components of the complement pathways (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). When an additional complement C3b molecule binds to the C3 convertase, forms the C5 convertase, which cleaves and activates C5 into C5a anaphylatoxin and C5b component of the membrane attack complex (PubMed : 30643019, PubMed : 624565, PubMed : 6554279).. Complement factor B Ba. Involved in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes.
See full target information CFB
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com