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Human Complement Factor B (Ba fragment) ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Complement Factor B (Ba fragment) in Serum, EDTA Plasma, Heparin Plasma, Cell culture supernatant samples.
Alternative names=Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, PBF2, Properdin factor B, GBG, CFB, BF, BFD
- Detection method
Colorimetric
- Sample types
Serum, EDTA Plasma, Heparin Plasma, Cell culture supernatant
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
21.875 - 1400 pg/mL
- Assay time
1h 30m
- Sensitivity
= 12.9 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Human Complement Factor B (Ba fragment) ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Complement Factor B (Ba fragment) in Serum, EDTA Plasma, Heparin Plasma, Cell culture supernatant samples.
Alternative names=Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, PBF2, Properdin factor B, GBG, CFB, BF, BFD
Key facts
- Detection method
Colorimetric
- Sample types
Serum, EDTA Plasma, Heparin Plasma, Cell culture supernatant
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
21.875 - 1400 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 12.9 pg/mL
- General recovery
102%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 8 | mean - | SD - | C.V. 8.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 3 | mean - | SD - | C.V. 3.03 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 102 | Range 99 - 106 % |
Sample type EDTA Plasma | Average % = 85 | Range 80 - 90 % |
Sample type Heparin Plasma | Average % = 92 | Range 91 - 92 % |
Sample type Cell culture supernatant | Average % = 90 | Range 86 - 93 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human Complement Factor B (Ba fragment) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Complement Factor B (Ba fragment) protein in Serum, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human Complement Factor B (Ba fragment) with 12.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Product protocols
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