Human Complement Factor B (Ba fragment) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Complement Factor B (Ba fragment) with a sensitivity of 12.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Heparin Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 21.875 - 1400 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 12.9 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
Alternative names
BF, BFD, CFB, Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, PBF2, Properdin factor B, GBG
What's included?
Recommended products
Human Complement Factor B (Ba fragment) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Complement Factor B (Ba fragment) with a sensitivity of 12.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Heparin Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 21.875 - 1400 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 12.9 pg/mL
- General recovery
- 102%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Plasma | n 8 | C.V. 8.1 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Plasma | n 3 | C.V. 3.03 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 102 | Range 99 - 106 % |
Sample type EDTA Plasma | Average % = 85 | Range 80 - 90 % |
Sample type Heparin Plasma | Average % = 92 | Range 91 - 92 % |
Sample type Cell culture supernatant | Average % = 90 | Range 86 - 93 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Complement Factor B (Ba fragment) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Complement Factor B (Ba fragment) protein in Serum, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human Complement Factor B (Ba fragment) with 12.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Complement Factor B also known as factor B complement is an important protein in the immune system. It weighs about 93 kDa and mainly expresses in the liver though it also circulates in the blood. This protein plays an important role in the alternative pathway of complement activation. Upon activation factor B combines with complement C3b to form the C3bB complex which then turns into the C3 convertase. This converts more C3 into C3b amplifying the immune response.
- Biological function summary
Complement Factor B serves as a serine protease that is essential in the body's defense mechanisms. It is an integral part of the C3/C5 convertase enzyme complex. When C3b binds to factor B complement it splits into Ba and Bb fragments. The Bb fragment becomes part of the C3 convertase structure on microbial surfaces facilitating greater immune defense. Factor B's action allows the body to rapidly respond to pathogens contributing to innate immunity by helping opsonize and clear pathogens.
- Pathways
This protein participates chiefly in the alternative complement pathway. It interacts closely with other complement components such as factor C3 and factor D to promote this pathway. The cascade initiated by complement factor B targets pathogens for destruction and assists in inflammation processes. It ensures a quick response in eliminating infectious agents before the adaptive immune system gets activated linking the innate and adaptive immune responses.
- Associated diseases and disorders
Research links Complement Factor B to conditions like age-related macular degeneration (AMD) and atypical hemolytic uremic syndrome (aHUS). In these disorders abnormal regulation or mutations in factor B can lead to excessive complement activation. For AMD this hyperactivity results in damage to retinal cells whereas in aHUS it causes issues in blood vessels and kidneys. Decay-accelerating factor and factor H also play notable roles here regulating this pathway to prevent overactivation that could harm healthy tissues.
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4 product images
Sandwich ELISA - Human Complement Factor B (Ba fragment) ELISA Kit (ab315062)
Example of human Complement Factor B (Ba Fragment) standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Complement Factor B (Ba fragment) ELISA Kit (ab315062)
Interpolated concentration of native Complement Factor B (Ba Fragment) was measured in duplicate. Undiluted samples are as follows: HepG2 cell culture supernatant 1:40. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Complement Factor B (Ba fragment) ELISA Kit (ab315062)
Interpolated concentration of native Complement Factor B (Ba Fragment) was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 1:4,000, plasma (EDTA) 1:200, and plasma (heparin) 1:4,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Complement Factor B (Ba fragment) ELISA Kit (ab315062)
Serum of eight individual healthy human donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor B (Ba Fragment) concentration was determined to be 2,129 ng/mL with a range of 1,133 – 4,554 ng/mL.
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