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AB243686

Human Complement Factor D ELISA Kit

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(1 Publication)

Human Complement Factor D ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Complement Factor D with a sensitivity of 2.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

DF, PFD, CFD, Complement factor D, Adipsin, C3 convertase activator, Properdin factor D

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Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)

Interpolated concentrations of native Complement Factor D in human serum and plasma samples.

The concentrations of Complement Factor D were measured in duplicates, interpolated from the Complement Factor D standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 1,000, plasma (citrate) 1 : 1,000, plasma (EDTA) 1 : 1,000and plasma (heparin) 1 : 1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor D concentration was determined to be 944.1 ng/mL in serum, 721.8 ng/mL in plasma (citrate), 860.5 ng/mL in plasma (EDTA), and 321.7 ng/mL in plasma (heparin).

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)

Human Complement Factor D Standard Curve Comparison.

Standard Curve comparison between human Complement Factor D SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor D concentration was determined to be 975.9 ng/mL with a range of 556.8 – 1,603.5 ng/mL.

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)
  • sELISA

Unknown

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)

Example of human Complement Factor D standard curve in Sample Diluent NS.

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)

Interpolated concentrations of native Complement Factor D in human urine and cell culture supernatant samples.

The concentrations of Complement Factor D were measured in duplicates, interpolated from the Complement Factor D standard curves and corrected for sample dilution. Undiluted samples are as follows : male urine 100%, female urine 50%, and U937 supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor D concentration was determined to be 958.8 pg/mL in male urine, 494.7 pg/mL in female urine and 4,734.2 pg/mL in U937 supernatant.

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor D concentration was determined to be 1,142.5 ng/mL with a range of 867.1 – 1,958.8 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.6 pg/mL

Range

15.63 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Complement Factor D ELISA Kit (ab243686) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Complement Factor D protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Complement Factor D with 2.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "87 - 102 %", "average": "= 96" }, { "sample": "Serum", "range": "92 - 105 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "84 - 102 %", "average": "= 93" }, { "sample": "Urine", "range": "83 - 90 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "82 - 102 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "90 - 100 %", "average": "= 94" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab243686-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB243686-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB243686-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB243686-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB243686-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB243686-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Complement Factor D Lyophilized Recombinant Protein", "number":"AB243686-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB243686-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Complement Factor D Capture Antibody", "number":"AB243686-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Complement Factor D Detector Antibody", "number":"AB243686-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB243686-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor D also known as complement factor D or CFD protein is a serine protease involved in the alternative pathway of the complement system. It plays a mechanical role by cleaving factor B when it is bound to C3b forming the active C3 convertase C3bBb. This enzyme is relatively small with a mass of approximately 28.5 kDa. It is mainly expressed in adipose tissue but it can also be found in the blood plasma.
Biological function summary

Factor D aids the immune system's ability to destroy pathogens. As part of the alternative complement pathway it acts independently and does not associate with larger protein complexes. Its activation of the alternative pathway leads to direct lysis of pathogens therefore contributing to innate immunity.

Pathways

Factor D participates specifically in the complement activation pathway particularly in the innate immune response. It works closely with other proteins in the complement system such as factor B and C3. This pathway enhances the opsonization and removal of microbes and damaged cells acting in parallel with the classical pathway of the complement system.

Abnormal function or regulation of factor D can relate to chronic inflammation and age-related macular degeneration. Disorders involving factor D may involve excess complement activation leading to tissue damage. The role of factor D in these diseases often connects it to other key complement components such as C3 and factor B which can also become problematic when dysregulated.
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Product protocols

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Target data

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
See full target information CFD
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cardiovascular medicine 9:1003282 PubMed36172581

2022

Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wanrong Man,Xinglong Song,Zhenyu Xiong,Jing Gu,Jie Lin,Xiaoming Gu,Duan Yu,Congye Li,Mengyuan Jiang,Xuebin Zhang,Zhi Yang,Yang Cao,Yan Zhang,Xiaofei Shu,Dexi Wu,Haichang Wang,Gang Ji,Dongdong Sun
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