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AB252359

Human Complement factor H ELISA Kit

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(4 Publications)

Human Complement factor H ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Complement factor H with a sensitivity of 74 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

HF, HF1, HF2, CFH, Complement factor H, H factor 1

5 Images
ELISA - Human Complement factor H ELISA Kit (AB252359)
  • ELISA

Supplier Data

ELISA - Human Complement factor H ELISA Kit (AB252359)
Sandwich ELISA - Human Complement factor H ELISA Kit (AB252359)
  • sELISA

Unknown

Sandwich ELISA - Human Complement factor H ELISA Kit (AB252359)

Interpolated concentrations of native Complement Factor H in human saliva and A549 cell culture supernatant samples.

The concentrations of Complement Factor H were measured in duplicates, interpolated from the Complement Factor H standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva, 2.5% and A549 cell culture supernatant, 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor H concentration was determined to be 179 ng/mL in saliva and 31 ng/mL in A549 cell culture supernatant.

Sandwich ELISA - Human Complement factor H ELISA Kit (AB252359)
  • sELISA

Unknown

Sandwich ELISA - Human Complement factor H ELISA Kit (AB252359)

Example of human Complement Factor H standard curve in Sample Diluent NS.

The Complement Factor H standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Complement factor H ELISA Kit (AB252359)
  • sELISA

Unknown

Sandwich ELISA - Human Complement factor H ELISA Kit (AB252359)

Interpolated concentrations of native Complement Factor H in human serum and plasma samples.

The concentrations of Complement Factor H were measured in duplicates, interpolated from the Complement Factor H standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 50,000, plasma (citrate) 1 : 50,00, plasma (EDTA) 1 : 50,000, and plasma (heparin) 1 : 50,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor H concentration was determined to be 268 μg/mL in serum, 247 μg/mL in plasma (citrate), 231 μg/mL in plasma (EDTA), and 187 μg/mL in plasma (heparin).

Sandwich ELISA - Human Complement factor H ELISA Kit (AB252359)
  • sELISA

Unknown

Sandwich ELISA - Human Complement factor H ELISA Kit (AB252359)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor H concentration was determined to be 288 μg/mL with a range of 156.1 – 466.5 μg/mL.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 74 pg/mL

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Complement factor H ELISA Kit ab252359 is a rapid single-wash 90-min Sandwich ELISA to measure Human Complement factor H in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, saliva, serum. This SimpleStep sensitivity is 74 pg/mL.

How the assay works

Human Complement factor H SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Complement factor H ELISA Kit ab252359 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Factor H antibody [EPR22253-144] - BSA and Azide free (Capture) ab259654
- Anti-Factor H antibody [EPR22253-117] - BSA and Azide free (Detector) ab259654
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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "102 - 113 %", "average": "= 106" }, { "sample": "Serum", "range": "101 - 113 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "100 - 110 %", "average": "= 105" }, { "sample": "Saliva", "range": "97 - 110 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "104 - 113 %", "average": "= 110" }, { "sample": "Citrate plasma", "range": "113 - 119 %", "average": "= 115" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor H also known as complement factor H is an important regulatory protein in the complement system. It has a molecular mass of approximately 155 kDa. This protein is mainly expressed in the liver but it can also be found in low levels in other tissues. Factor H serves as a control element for complement activation particularly affecting the alternative pathway. It binds to C3b a central component of the complement system and accelerates the decay of C3 convertase as well as promotes the proteolytic inactivation of C3b by factor I.
Biological function summary

Factor H limits the activity of the complement system to prevent damage to host tissues. The protein exists in the plasma in a soluble form. It functions by recognizing host cell surfaces via specific markers avoiding inappropriate activation. Factor H belongs to a group of proteins which include other regulators of complement activation. These proteins maintain the balance between effective immune defense and protection of host tissue from excessive immune responses.

Pathways

Factor H is a part of the alternative complement pathway. This pathway is important for innate immune response involving proteins like factor P (properdin) which stabilizes C3 convertase. Factor H modulates these interactions to prevent unwarranted complement activity on host cells. Another related pathway is the classic complement pathway although factor H's involvement here is less direct since it primarily regulates the alternative pathway.

Factor H associations include atypical hemolytic uremic syndrome and age-related macular degeneration. Factor H deficiency or dysfunction can lead to uncontrolled complement activation resulting in kidney damage in atypical hemolytic uremic syndrome where it is also related to factor I. Additionally in age-related macular degeneration variants in the factor H gene are linked to increased susceptibility further highlighting the protein's importance in regulating immune responses.
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Product protocols

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Target data

Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces (PubMed : 21285368, PubMed : 25402769). Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop (PubMed : 19503104, PubMed : 26700768). As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b (PubMed : 18252712, PubMed : 23332154, PubMed : 28671664). In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed (PubMed : 20008295, PubMed : 9558116).. (Microbial infection) In the mosquito midgut, binds to the surface of parasite P.falciparum gametocytes and protects the parasite from alternative complement pathway-mediated elimination.
See full target information CFH
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1013107 PubMed40294075

2025

Deposition of complement regulators on the surface of Plasmodium falciparum merozoites depends on the immune status of the host.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Rosaria Bassi,Bogdan Cristinoi,Frank Buitenwerf,Mark Bergholt Cuadrado,Kasper Haldrup Björnsson,Melanie Rose Walker,Frederica Dedo Partey,Andrew B Ward,Michael Fokuo Ofori,Lea Barfod

Frontiers in immunology 15:1320094 PubMed38576611

2024

Differentiated pattern of complement system activation between MOG-IgG-associated disease and AQP4-IgG-positive neuromyelitis optica spectrum disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Bin Cho,Ju-Hong Min,Patrick Waters,Miyoung Jeon,Eun-Seon Ju,Ho Jin Kim,Su-Hyun Kim,Ha Young Shin,Sa-Yoon Kang,Young-Min Lim,Sun-Young Oh,Hye Lim Lee,Eunhee Sohn,Sang-Soo Lee,Jeeyoung Oh,Sunyoung Kim,So-Young Huh,Joong-Yang Cho,Jin Myoung Seok,Byung-Jo Kim,Byoung Joon Kim

Investigative ophthalmology & visual science 63:10 PubMed35426907

2022

Targeted Analysis of Tears Revealed Specific Altered Metal Homeostasis in Age-Related Macular Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Valencia,Montserrat García,Beatriz Fernández-Vega,Rosario Pereiro,Lara Lobo,Héctor González-Iglesias

ACS chemical neuroscience 13:1030-1045 PubMed35200010

2022

Fibrinogen and Complement Factor H Are Promising CSF Protein Biomarkers for Parkinson's Disease with Cognitive Impairment─A Proteomics-ELISA-Based Study.

Applications

Unspecified application

Species

Unspecified reactive species

Aditi Naskar,Albert Stezin,Arpitha Dharmappa,Shantala Hegde,Mariamma Philip,Nitish Kamble,Jitender Saini,K Sandhya,Utpal Tatu,Ravi Yadav,Pramod Kumar Pal,Phalguni Anand Alladi
View all publications
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