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AB195460

Human Complement Factor I ELISA Kit

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(5 Publications)

Human Complement Factor I ELISA Kit is a Competitive ELISA for the measurement of Human Complement Factor I in Human in Biofluids samples.

View Alternative Names

IF, CFI, Complement factor I, C3B/C4B inactivator

1 Images
Competitive ELISA - Human Complement Factor I ELISA Kit (AB195460)
  • cELISA

Supplier Data

Competitive ELISA - Human Complement Factor I ELISA Kit (AB195460)

Representative standard curve using ab195460.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Plasma, Milk, Serum

Reacts with

Human

Assay type

Competitive

Sensitivity

= 0.28 µg/mL

Range

0.375 - 24 µg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Complement Factor I Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement Factor I levels in plasma, serum, milk and saliva samples.

A Complement Factor I specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Complement Factor I is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Complement Factor I captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab195460-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB195460-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Complement Factor I Standard (Lyophilised)", "number":"AB195460-CMP04", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB195460-CMP07", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB195460-CMP08", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB195460-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"2X Biotinylated Complement Factor I (Lyophilised)", "number":"AB195460-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Complement Factor I Microplate (12 x 8 well strips)", "number":"AB195460-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent M Concentrate", "number":"AB195460-CMP01", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB195460-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor I also known as Complement Factor I (CFI) is a serine protease involved in the complement system. Its molecular mass is approximately 88 kDa consisting of a heavy and a light chain linked by a disulfide bond. This protein is mainly expressed in the liver and circulates in the plasma. Factor I inhibits the complement pathways by cleaving complement components C3b and C4b preventing the formation of C3 and C5 convertases.
Biological function summary

Factor I plays a role in maintaining immune homeostasis by preventing excessive complement activation. It acts within a complex that includes cofactors such as Factor H Complement Factor 4 binding protein (C4BP) and Membrane Cofactor Protein (MCP). These interactions allow Factor I to selectively degrade activated complement components ensuring that complement activity is kept in check to avoid host cell damage.

Pathways

Factor I operates in both the classical and alternative complement pathways. In the classical pathway it regulates C3b and C4b breakdown to control the formation of the C3 convertase. In the alternative pathway Factor I works in conjunction with Factor H to degrade C3b. This regulation is important to prevent runaway activation protecting healthy tissue from complement-mediated damage.

Factor I deficiencies can lead to conditions like atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD). In aHUS insufficient Factor I activity permits unregulated complement activation leading to thrombotic microangiopathy. In AMD aberrant complement activation contributes to retinal damage and degeneration. These conditions illustrate the need for balanced Factor I activity to prevent disease.
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Product protocols

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Target data

Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins (PubMed : 17320177, PubMed : 7360115). Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces (PubMed : 12055245, PubMed : 2141838, PubMed : 9605165). The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization (PubMed : 28671664).
See full target information CFI
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Proteomics. Clinical applications 18:e202300069 PubMed38332320

2024

Plasma proteome analysis and validation of patients with community-acquired pneumonia: A cohort study.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Zhao,Wenjie Bian,Ying Shang,Hui Zhi,Xinqian Ma,Yukun He,Wenyi Yu,Chunyu Liu,Yu Xu,Pihua Gong,Zhancheng Gao

Journal of clinical immunology 41:1607-1620 PubMed34232441

2021

Elevated Expression Levels of Lung Complement Anaphylatoxin, Neutrophil Chemoattractant Chemokine IL-8, and RANTES in MERS-CoV-Infected Patients: Predictive Biomarkers for Disease Severity and Mortality.

Applications

Unspecified application

Species

Unspecified reactive species

Maaweya E Hamed,Asif Naeem,Haitham Alkadi,Aref A Alamri,Ahmad S AlYami,Abdullah AlJuryyan,Wael Alturaiki,Mushira Enani,Samia T Al-Shouli,Abdullah M Assiri,Bandar Alosaimi

Frontiers in immunology 12:668725 PubMed34276659

2021

Complement Anaphylatoxins and Inflammatory Cytokines as Prognostic Markers for COVID-19 Severity and In-Hospital Mortality.

Applications

Unspecified application

Species

Unspecified reactive species

Bandar Alosaimi,Ayman Mubarak,Maaweya E Hamed,Abdullah Z Almutairi,Ahmed A Alrashed,Abdullah AlJuryyan,Mushira Enani,Faris Q Alenzi,Wael Alturaiki

Oncotarget 9:14862-14881 PubMed29599912

2018

Coagulation cascade and complement system in systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liang,Shang-Bo Xie,Chang-Hao Wu,Yuan Hu,Qin Zhang,Si Li,Yin-Guang Fan,Rui-Xue Leng,Hai-Feng Pan,Hua-Bao Xiong,Dong-Qing Ye

ACS infectious diseases 3:880-885 PubMed28862830

2017

Plasma Levels of Complement Factor I and C4b Peptides Are Associated with HIV Suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Boyue Wu,Zhengyu Ouyang,Christopher J Lyon,Wei Zhang,Tori Clift,Christopher R Bone,Boan Li,Zhen Zhao,Jason T Kimata,Xu G Yu,Ye Hu
View all publications
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