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AB284615

Human Complement Factor I ELISA Kit

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Human Complement Factor I ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Complement Factor I with a sensitivity of 0.17 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

IF, CFI, Complement factor I, C3B/C4B inactivator

5 Images
ELISA - Human Complement Factor I ELISA Kit (AB284615)
  • ELISA

Supplier Data

ELISA - Human Complement Factor I ELISA Kit (AB284615)
Sandwich ELISA - Human Complement Factor I ELISA Kit (AB284615)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement Factor I ELISA Kit (AB284615)

Interpolated concentrations of Complement Factor I in normal human serum donors.

Serum of eight individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Complement Factor I concentration was determined to be 24.6 mg/mL with a range of 16.7 – 34.3 mg/mL.

Sandwich ELISA - Human Complement Factor I ELISA Kit (AB284615)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement Factor I ELISA Kit (AB284615)

Interpolated concentrations of human Complement Factor I in serum, plasma (EDTA), plasma (citrate), and plasma (heparin).

Interpolated concentration of native Complement Factor I was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 2,000, plasma (EDTA) 1 : 2,000, plasma (citrate) 1 : 2,000, and plasma (heparin) 1 : 1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Complement Factor I ELISA Kit (AB284615)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement Factor I ELISA Kit (AB284615)

Interpolated concentrations of spiked human Complement Factor I in RPMI 10% FBS Media.

Recombinant Complement Factor I was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 50% RPMI1640 10% FBS Media. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Complement Factor I ELISA Kit (AB284615)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement Factor I ELISA Kit (AB284615)

Example of human Complement Factor I standard curve in Sample Diluent NS.

Example of human Complement Factor I standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.17 ng/mL

Range

0.313 - 20 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Complement Factor I ELISA Kit ab284615 is a rapid single-wash 90-min Sandwich ELISA to measure Human Complement Factor I in cell culture media, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 0.17 ng/mL.

How the assay works

Human Complement Factor I SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Complement Factor I ELISA Kit ab284615 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.1" } ]
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Recovery

[ { "sample": "Serum", "range": "109 - 117 %", "average": "= 112" }, { "sample": "EDTA Plasma", "range": "107 - 113 %", "average": "= 110" }, { "sample": "Cell culture media", "range": "85 - 105 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "97 - 108 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "105 - 118 %", "average": "= 111" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab284615-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB284615-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB284615-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB284615-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB284615-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB284615-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB284615-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Complement Factor I Capture Antibody", "number":"AB284615-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Complement Factor I Lyophilized Recombinant Protein", "number":"AB284615-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Complement Factor I Detector Antibody", "number":"AB284615-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB284615-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement Factor I (CFI) also known as C3b/C4b inactivator Complement Factor I and Factor I Complement is a serine protease with a mass of approximately 88 kDa. It is expressed in the liver and secreted into the bloodstream. This protein cleaves complement components C3b and C4b preventing the assembly of C3/C5 convertase. As a regulator CFI ensures the complement system does not damage host tissues by maintaining it in check.
Biological function summary

Complement Factor I plays a significant role in controlling complement activation. It operates as part of a regulatory complex with cofactors that include Factor H C4-binding protein and membrane cofactor protein (MCP/CD46). By inactivating C3b and C4b fragments CFI helps prevent the formation of the terminal complement complex offering protection against excessive immune response and potential tissue damage. This regulation is essential for maintaining homeostasis within the immune system.

Pathways

Complement Factor I is involved in both the alternative and classical pathways of complement activation. It relates closely to proteins like Factor H and C3 convertase as its actions are vital in the degradation of C3b to iC3b and C4b to inactive forms. This regulatory mechanism ensures proper immune surveillance and prevention of autoimmune reactions by controlling the extent of complement activation on cell surfaces.

Deficiencies or mutations in Complement Factor I are connected to conditions such as atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD). These disorders arise due to dysregulation of the complement system where CFI links to proteins like Factor H and C3 impacting disease progression. Proper function of CFI in these diseases serves to protect tissues from the immune overactivation that leads to disease symptoms.
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Product protocols

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Target data

Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins (PubMed : 17320177, PubMed : 7360115). Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces (PubMed : 12055245, PubMed : 2141838, PubMed : 9605165). The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization (PubMed : 28671664).
See full target information CFI
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websiteProtocolBooklet
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