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AB213765

Human Complement H ELISA Kit

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(1 Publication)

Human Complement H ELISA Kit is a Sandwich ELISA for the measurement of Human Complement H in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

HF, HF1, HF2, CFH, Complement factor H, H factor 1

1 Images
Sandwich ELISA - Human Complement H ELISA Kit (AB213765)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement H ELISA Kit (AB213765)

Human Complement H ELISA Kit (ab213765) Standard Curve

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, EDTA Plasma, Heparin Plasma, Plasma, Serum, Urine

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 50 pg/mL

Range

1.56 - 100 ng/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

The Human Complement H Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213765) is designed for the quantitative measurement Human Complement H/CFH in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

The kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Complement H/CFH has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Complement H/CFH is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Complement H/CFH amount of sample captured in plate.

Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternative complement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "3.406 ng/mL", "standardDeviation": "0.303", "coefficientOfVariability": "8.9" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "12.935 ng/mL", "standardDeviation": "0.905", "coefficientOfVariability": "7" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "57.149 ng/mL", "standardDeviation": "4.972", "coefficientOfVariability": "8.7" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "3.203 ng/mL", "standardDeviation": "0.234", "coefficientOfVariability": "7.3" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "13.518 pg/mL", "standardDeviation": "0.754", "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "53.844 ng/mL", "standardDeviation": "3.823", "coefficientOfVariability": "6.3" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor H also known as complement factor H is an important regulatory protein in the complement system. It has a molecular mass of approximately 155 kDa. This protein is mainly expressed in the liver but it can also be found in low levels in other tissues. Factor H serves as a control element for complement activation particularly affecting the alternative pathway. It binds to C3b a central component of the complement system and accelerates the decay of C3 convertase as well as promotes the proteolytic inactivation of C3b by factor I.
Biological function summary

Factor H limits the activity of the complement system to prevent damage to host tissues. The protein exists in the plasma in a soluble form. It functions by recognizing host cell surfaces via specific markers avoiding inappropriate activation. Factor H belongs to a group of proteins which include other regulators of complement activation. These proteins maintain the balance between effective immune defense and protection of host tissue from excessive immune responses.

Pathways

Factor H is a part of the alternative complement pathway. This pathway is important for innate immune response involving proteins like factor P (properdin) which stabilizes C3 convertase. Factor H modulates these interactions to prevent unwarranted complement activity on host cells. Another related pathway is the classic complement pathway although factor H's involvement here is less direct since it primarily regulates the alternative pathway.

Factor H associations include atypical hemolytic uremic syndrome and age-related macular degeneration. Factor H deficiency or dysfunction can lead to uncontrolled complement activation resulting in kidney damage in atypical hemolytic uremic syndrome where it is also related to factor I. Additionally in age-related macular degeneration variants in the factor H gene are linked to increased susceptibility further highlighting the protein's importance in regulating immune responses.
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Product protocols

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Target data

Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces (PubMed : 21285368, PubMed : 21317894, PubMed : 25402769). Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop (PubMed : 19503104, PubMed : 21317894, PubMed : 26700768). As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b (PubMed : 18252712, PubMed : 23332154, PubMed : 28671664). In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed (PubMed : 20008295, PubMed : 9558116).. (Microbial infection) In the mosquito midgut, binds to the surface of parasite P.falciparum gametocytes and protects the parasite from alternative complement pathway-mediated elimination.
See full target information CFH
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of pediatric urology 16:466.e1-466.e9 PubMed32620509

2020

A novel urinary biomarker protein panel to identify children with ureteropelvic junction obstruction - A pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Charan Kumar V Devarakonda,Emily R Shearier,Chaoran Hu,James Grady,Jeremy L Balsbaugh,John H Makari,Fernando A Ferrer,Linda H Shapiro
View all publications
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