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Human COMT ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human COMT in Human in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.

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Sandwich ELISA - Human COMT ELISA Kit (AB213766), expandable thumbnail

Key facts

Detection method
Colorimetric
Sample types
Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
31.2 - 2000 pg/mL
Assay time
3h 30m
Sensitivity
< 10 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.

Alternative names

Catechol O-methyltransferase, COMT

What's included?

1 x 96 Tests
Components
ABC Diluent Buffer
1 x 12 mL
Adhesive Plate Seal
4 x 1 Unit
Anti-Human COMT coated Microplate (12 x 8 wells)
1 x 1 Unit
Antibody Diluent Buffer
1 x 12 mL
Avidin-Biotin-Peroxidase Complex (ABC)
1 x 100 µL
Biotinylated anti- Human COMT antibody
1 x 100 µL
Lyophilized recombinant Human COMT standard
2 x 1 Vial
Sample Diluent Buffer
1 x 30 mL
TMB Color Developing Agent
1 x 10 mL
TMB Stop Solution
1 x 10 mL
Wash Buffer (25X)
1 x 20 mL

Recommended products

Human COMT ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human COMT in Human in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
31.2 - 2000 pg/mL
Assay time
3h 30m
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
< 10 pg/mL

Precision

Intra assay

Sample
Sample 2
n
16
mean
245 pg/mL
SD
16.17
C.V.
= 6.6
Sample
Sample 1
n
16
mean
71 pg/mL
SD
4.97
C.V.
= 7
Sample
Sample 3
n
16
mean
923 pg/mL
SD
61.84
C.V.
= 6.7

Inter assay

Sample
Sample 3
n
24
mean
946 pg/mL
SD
79.46
C.V.
= 8.4
Sample
Sample 1
n
24
mean
65 pg/mL
SD
8.43
C.V.
= 7.9
Sample
Sample 2
n
24
mean
220 pg/mL
SD
16.5
C.V.
= 7.5

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

The Human COMT Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213766) is designed for the quantitative measurement of Human COMT in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for COMT has been pre-coated onto 96-well plates. Standards (E.coli, G52-P271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for COMT is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human COMT amount of sample captured in plate.

Catechol-O-methyltransferase (COMT) is one of several enzymes that degrade catecholamines such as dopamine, epinephrine, and norepinephrine. In humans, catechol-O-methyltransferase protein is encoded by the COMT gene. As the regulation of catecholamines is impaired in a number of medical conditions, several pharmaceutical drugs target COMT to alter its activity and therefore the availability of catecholamines. In the brain, COMT-dependent dopamine degradation is of particular importance in brain regions with low expression of the presynaptic dopamine transporter (DAT), such as the prefrontal cortex. This process is supposed to take place in postsynaptic neurons, as, in general, COMT is located intracellularly in the central nervous system (CNS).

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

COMT also known as catechol-O-methyltransferase is an enzyme responsible for the methylation of catechol compounds including neurotransmitters like dopamine epinephrine and norepinephrine. This reaction requires S-adenosylmethionine as a methyl donor. COMT primarily has two forms: a soluble form and a membrane-bound form with masses around 24 kDa and 30 kDa respectively. This protein shows significant expression in the liver brain and kidneys. COMT expression is also noted in cell lines such as HEK293T where it plays an important role in catecholamine metabolism.

Biological function summary

Catechol-O-methyltransferase serves as a regulator of neurotransmitter levels in the central nervous system and peripheral tissues. It does not appear to form part of a larger protein complex functioning mainly as a singular entity for metabolizing catechols. Through its action COMT impacts processes such as mood regulation cognition and stress response. Its role in degrading dopamine is especially significant in regions like the prefrontal cortex where dopamine plays an important role in executive functions.

Pathways

COMT participates in the catecholamine degradation pathway and impacts dopaminergic signaling. It works alongside enzymes such as monoamine oxidase (MAO) to regulate levels of neurotransmitters. COMT's activity influences the synaptic presence of dopamine and its interaction in signaling pathways. Moreover it indirectly affects the balance within the dopaminergic system which is important for normal neurological function and behavior.

Associated diseases and disorders

COMT has links to psychiatric and neurological conditions such as schizophrenia and Parkinson’s disease. Variations in COMT function can affect dopamine metabolism which may contribute to the etiology of these disorders. Additionally proteins like MAO and dopamine transporter (DAT) also connect to COMT through their shared involvement in neurotransmitter regulation. Alterations in COMT activity can influence disease severity and patient response to treatment underlining its importance in understanding these disorders.

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  • Sandwich ELISA - Human COMT ELISA Kit (ab213766), expandable thumbnail

    Sandwich ELISA - Human COMT ELISA Kit (ab213766)

    Human COMT ELISA Kit (ab213766) Standard Curve.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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