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AB313536

Human Contactin 1 ELISA Kit

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Human Contactin 1 ELISA Kit is a Sandwich ELISA for the measurement of Human Contactin 1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Contactin-1, Glycoprotein gp135, Neural cell surface protein F3, CNTN1

1 Images
Sandwich ELISA - Human Contactin 1 ELISA Kit (AB313536)
  • sELISA

Supplier Data

Sandwich ELISA - Human Contactin 1 ELISA Kit (AB313536)

Example of standard curve using ab313536. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.06 ng/mL

Range

0.06 - 15 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Contactin 1 (CNTN1) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Contactin 1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Contactin 1 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "127 - 150 %", "average": "= 140.1" }, { "sample": "Plasma", "range": "119 - 134 %", "average": "= 125.4" }, { "sample": "Cell culture media", "range": "105 - 113 %", "average": "= 109.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313536-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313536-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313536-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313536-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313536-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Contactin 1 Protein Standard", "number":"AB313536-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin", "number":"AB313536-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Contactin 1 Detection Antibody", "number":"AB313536-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Contactin 1 Antibody-coated ELISA Plate", "number":"AB313536-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Contactin 1 also known as contactin-1 or contactin is a protein involved in cell adhesion processes. It is sometimes referred to as contacton. This protein has a molecular weight of approximately 120 kDa and is a neural membrane glycoprotein. Contactin 1 primarily expresses in the developing nervous system and persists in the adult central nervous system. It plays an important role in forming connections between neurons and ensuring proper communication between them.
Biological function summary

The role of this protein involves maintaining neuronal network organization and integrity. Contactin 1 plays a critical part in myelination a process essential for the swift conduction of nerve impulses. It interacts with several partners forming complexes essential for neuronal stability including neurofascin and contactin-associated protein-like 2. These interactions facilitate proper signal transmission across synapses in the nervous system.

Pathways

Contactin 1 is an important element in the pathways that regulate neuronal development and myelination. It is involved in the formation of the nodes of Ranvier important for nerve impulse transmission. Contactin 1 interacts with proteins such as tenascin-R and NrCAM in these processes contributing significantly to the formation and maintenance of functional neural networks.

Defects in the regulation or expression of contactin 1 are associated with neurological conditions like multiple sclerosis and Guillain-Barré syndrome. These disorders highlight problems in nerve signal transmission and immune system misregulation. Through these diseases contactin 1's interaction with proteins such as CASPR (contactin-associated protein-like 2) becomes important as disruptions in these interactions can lead to neuronal dysfunction.
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Product protocols

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Target data

Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth (By similarity).
See full target information CNTN1
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websiteProtocolBooklet
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Product promise

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