Human Contactin 2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Contactin 2 in Human in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.102 - 25 ng/mL
- Sensitivity
- = 0.12 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
In conjunction with another transmembrane protein, CNTNAP2, contributes to the organization of axonal domains at nodes of Ranvier by maintaining voltage-gated potassium channels at the juxtaparanodal region. May be involved in cell adhesion.
Alternative names
AXT, TAG1, TAX1, CNTN2, Contactin-2, Axonal glycoprotein TAG-1, Axonin-1, Transient axonal glycoprotein 1, TAX-1
What's included?
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Human Contactin 2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Contactin 2 in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.102 - 25 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.12 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 141.9 | Range 137 - 147 % |
Sample type Serum | Average % = 117.9 | Range 99 - 135 % |
Sample type Plasma | Average % = 120.8 | Range 84 - 138 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human Contactin 2 ELISA Kit is designed for the quantitative determination of Contactin 2 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human Contactin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Contactin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Contactin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Contactin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ALK-1, B7-H2, BLAME, BMP-8, CD28, Common beta Chain, Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-12, FGF17, FOLR2, Galectin-8, Glypican 5, GHR, IFN-gamma R1, IL-22 R alpha 1, IL-22BP, IL-23 R, IL-31 RA, IL-7 R alpha, Integrin alpha 5, MDM2, Nectin-1, NKp30, Nogo,Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 6D, Semaphorin 7A, Siglec-11.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TAG1 also known as TAX1 or contactin-2 is a protein encoded by the CNTN2 gene in humans. It is a member of the immunoglobulin superfamily and has a mass of approximately 140 kDa. TAG1 is expressed in the nervous system particularly in the peripheral and central nervous systems during development. It is predominantly found on neurons influencing axonal guidance and neuronal connectivity.
- Biological function summary
TAG1 plays a fundamental role in the nervous system by mediating cell adhesion and signaling. It forms part of a complex often interacting with other cell adhesion molecules to facilitate cellular communication and neurite outgrowth. By influencing neuronal positioning and patterning TAG1 contributes to the proper formation of neural circuits.
- Pathways
There is a strong involvement of TAG1 in axonal growth and guidance pathways as well as in the cell adhesion signaling pathway. It directly interacts with proteins such as Caspr and neurofascin which are critical for myelination and the maintenance of the node of Ranvier. These interactions help guide neuron development and function.
- Associated diseases and disorders
Altered expression of TAG1 has been linked to several neural disorders including autism spectrum disorders and schizophrenia. In the context of these diseases TAG1 interacts with other proteins such as contactin-associated protein 1 (Caspr1) which plays an important role in maintaining neural network stability and function. Understanding these interactions provides insights into the pathogenesis of these neurological conditions.
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1 product image
Sandwich ELISA - Human Contactin 2 ELISA Kit (ab267632)
These standard curves are for demonstration only. A standard curve must be run with each assay.
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Product protocols
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