Human CoREST ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CoREST in Human in Serum, Plasma, Cell culture supernatant samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 7 - 1500 pg/mL
- Sensitivity
- = 7 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation.
Alternative names
KIAA0071, RCOR, RCOR1, REST corepressor 1, Protein CoREST
What's included?
Recommended products
Human CoREST ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CoREST in Human in Serum, Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 7 - 1500 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 7 pg/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 106.2 | Range 76 - 142 % |
Sample type Plasma | Average % = 78.64 | Range 72 - 93 % |
Sample type Cell culture media | Average % = 119.9 | Range 90 - 143 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human CoREST ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human RCOR1 in serum (human CoREST concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human CoREST coated on a 96-well plate.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Coagulation Factor X, CHMP2B, Contactin-3, Cortactin, CrkL, Cyr61/CCN1, DAPP1, Dynactin Subunit 1/DCTN1, DFF45/ICAD, DRAK1, GRAP2, GRK5, Hydroxyacid Oxidase1/HAO-1, LRIG1, MMP-12, NCK1, Nectin-2/CD112, Nesfatin-1/Nucleobindin-2, Neurogranin, Nrf2, NUDT5, PAR1, Pancreatic Polypeptide/PP, Peroxiredoxin 2, PSMA1, PU.1/Spi-1, RalA, Serpin B8, SH2D1A, SHP-1, Siglec-6/CD327, SorCS3, THAP11, ULBP-4/RAET1E, UNC5H3, VAMP-1, VAMP-2, PBEF/Visfatin.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CoREST protein also known as REST corepressor 1 is a 58 kDa protein that mechanically functions in chromatin remodeling and gene silencing. CoREST localizes to the nucleus and functions in the repression of neuronal genes in non-neuronal tissues. This protein is pivotal in assembling histone deacetylase complexes which makes it essential in epigenetic regulation. CoREST is expressed in various tissues throughout the body playing an important role in maintaining non-neuronal gene expression.
- Biological function summary
CoREST interacts with multiple partners to form a corepressor complex. This complex includes LSD1 (lysine-specific demethylase 1) and HDAC1/2 (histone deacetylases 1 or 2) facilitating transcriptional repression. CoREST regulates the expression of genes involved in neurogenesis and cell differentiation processes. Its role is critical in maintaining the balance between neuronal and non-neuronal gene expression by modifying chromatin states.
- Pathways
CoREST is involved in histone modification and gene expression regulation pathways. One significant pathway is the REST (RE1-Silencing Transcription factor) pathway where CoREST serves as a core component. In this pathway CoREST interacts with REST a transcriptional repressor to silence neural genes in a non-neuronal context. Another involved pathway is the chromatin remodeling pathway where CoREST associates with LSD1 and other chromatin-modifying enzymes to alter chromatin structure and gene accessibility.
- Associated diseases and disorders
CoREST has connections to neurological disorders and cancer. Altered CoREST expression or function links to the progression of neurodegenerative diseases such as Huntington's disease. CoREST dysregulation also relates to certain cancer types where misexpression can contribute to tumorigenesis. In the context of these disorders CoREST often collaborates with LSD1 affecting the transcriptional landscape of affected cells showcasing the importance of CoREST in disease mechanisms.
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1 product image
Sandwich ELISA - Human CoREST ELISA Kit (ab314376)
Example of standard curve using a314376. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
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