Human Coronin 1a ELISA Kit
- Recombinant
- SimpleStep
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(1 Publication)
Human Coronin 1a ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Coronin 1a with a sensitivity of 49 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
CORO1, CORO1A, Coronin-1A, Coronin-like protein A, Coronin-like protein p57, Tryptophan aspartate-containing coat protein, Clipin-A, TACO
- sELISA
Supplier Data
Sandwich ELISA - Human Coronin 1a ELISA Kit (AB214032)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Coronin 1a concentration was determined to be 85.97 ng/mL with a range of 4.85 – 716.8 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Coronin 1a ELISA Kit (AB214032)
Example of Human Coronin 1a standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Coronin 1a ELISA Kit (AB214032)
Interpolated concentrations of native Coronin 1a in human HEPG2, MCF7, and Jurkat cell extract samples and samples based on a 400 μg/mL, 200 µg/mL, and 8 µg/mL extract load respectively.
The concentrations of Coronin 1a were measured in duplicate and interpolated from the Coronin 1a standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Coronin 1a concentration was determined to be 21.4 pg/μg HEPG2 extract, 54.4 pg/ μg MCF7 extract and 888.5 pg /μg Jurkat extract.
- sELISA
Supplier Data
Sandwich ELISA - Human Coronin 1a ELISA Kit (AB214032)
Interpolated concentrations of native Coronin 1a in human Peripheral Blood Monocyte Cell (PBMC) culture supernatant samples.
PBMC cells were grown in the absence (unstimulated) or presence of Phorbol Myistrate Acetate (PMA and phytohemagglutinin (PHA) (stimulated) for 3 days. The concentrations of Coronin 1a were measured in duplicates, interpolated from the Coronin 1a standard curves and corrected for sample dilution. Undiluted samples are as follows : unstimulated 50% and stimulated (25%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Coronin 1a concentration was determined to be 38.7 ng/mL in unstimulated and 76.9 ng/mL in stimulated, and 6.7ng/mL in media. This pair is highly cross reactive with bovine serum. Dilute bovine serum containing media such that the concentration of bovine serum is less than 0.1% or run a media control sample.
- sELISA
Supplier Data
Sandwich ELISA - Human Coronin 1a ELISA Kit (AB214032)
Example of Human Coronin 1a standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Human Coronin 1a ELISA Kit (ab214032) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Coronin 1a protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Coronin 1a with 49 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Coronin 1A is a WD-repeat containing protein that plays a role in cytoskeleton dynamics. Coronin 1A binds to actin. Mouse and rat Coronin 1A have 95% and 93% protein sequence identity to human Coronin 1A.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Product protocols
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Publications (1)
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Journal of molecular medicine (Berlin, Germany) 100:1307-1319 PubMed35915349
2022
Applications
Unspecified application
Species
Unspecified reactive species
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