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AB300308

Human Coxsackie Adenovirus Receptor/hCAR ELISA Kit

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Human Coxsackie Adenovirus Receptor/hCAR ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Coxsackie Adenovirus Receptor/hCAR with a sensitivity of 2.45 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CAR, CXADR, Coxsackievirus and adenovirus receptor, hCAR, CVB3-binding protein, Coxsackievirus B-adenovirus receptor, HCVADR

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Sandwich ELISA - Human Coxsackie Adenovirus Receptor/hCAR ELISA Kit (AB300308)
  • sELISA

Supplier Data

Sandwich ELISA - Human Coxsackie Adenovirus Receptor/hCAR ELISA Kit (AB300308)

Interpolated concentrations of human Coxsackie Adenovirus Receptor/hCAR in Hela cell extract.

Interpolated concentration of native Coxsackie Adenovirus Receptor/hCAR was measured in duplicate at different sample concentrations. Undiluted samples are 100 μg/mL Hela cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Coxsackie Adenovirus Receptor/hCAR ELISA Kit (AB300308)
  • sELISA

Supplier Data

Sandwich ELISA - Human Coxsackie Adenovirus Receptor/hCAR ELISA Kit (AB300308)

Example of human Coxsackie Adenovirus Receptor/hCAR standard curve in 1X Cell Extraction Buffer PTR.

Example of human Coxsackie Adenovirus Receptor/hCAR standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.45 pg/mL

Range

25 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "86 - 96 %", "average": "= 92" } ]
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What's included?

{ "values": { "96Tests": { "sellingSize": "96 Tests", "publicAssetCode":"ab300308-96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB300308-CMP21", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB300308-CMP20", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB300308-CMP17", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB300308-CMP19", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB300308-CMP15", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB300308-CMP18", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Coxsackie Adenovirus Receptor/hCAR Lyophilized Recombinant Protein", "number":"AB300308-CMP14", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Coxsackie Adenovirus Receptor/hCAR Capture Antibody", "number":"AB300308-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB300308-CMP22", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB300308-CMP16", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Coxsackie Adenovirus Receptor/hCAR Detector Antibody", "number":"AB300308-CMP13", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Component of the epithelial apical junction complex that may function as a homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair.. (Microbial infection) Acts as a receptor for adenovirus type C.. (Microbial infection) Acts as a receptor for Coxsackievirus B1 to B6.
See full target information CXADR
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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