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AB309191

Human CPE ELISA Kit - Extracellular

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Human CPE ELISA Kit - Extracellular is a Sandwich (quantitative) ELISA for the measurement of Human CPE - Extracellular in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Carboxypeptidase E, CPE, Carboxypeptidase H, Enkephalin convertase, Prohormone-processing carboxypeptidase, CPH

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Sandwich ELISA - Human CPE ELISA Kit - Extracellular (AB309191)
  • sELISA

Supplier Data

Sandwich ELISA - Human CPE ELISA Kit - Extracellular (AB309191)

Interpolated concentration of native CPE was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human serum 100%, human plasma (Citrate) 100%, human plasma (Heparin) 100%, and HepG2 cell culture supernatant 0.6%.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human CPE ELISA Kit - Extracellular (AB309191)
  • sELISA

Supplier Data

Sandwich ELISA - Human CPE ELISA Kit - Extracellular (AB309191)

Example of human CPE standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CPE ELISA Kit - Extracellular (AB309191)
  • sELISA

Supplier Data

Sandwich ELISA - Human CPE ELISA Kit - Extracellular (AB309191)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CPE concentration was determined to be 7,394 pg/mL with a range of 5,911 – 8,814 pg/mL.

Key facts

Detection method

Colorimetric

Sample types

Serum, Heparin Plasma, Citrate plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 32 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CPE - Extracellular SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CPE - Extracellular protein in human serum, Hepain plasma, Citrate plasma and cell culture supernatant. Quantitate Human CPE - Extracellular with 19 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.1" } ]
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Recovery

[ { "sample": "Serum", "range": "89 - 106 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "80 - 95 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "87 - 94 %", "average": "= 90" }, { "sample": "Cell culture supernatant", "range": "82 - 99 %", "average": "= 92" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Carboxypeptidase E (CPE) also known as carboxypeptidase H is an enzyme with a mass of about 53 kDa. It facilitates the removal of C-terminal amino acid residues from proteins and peptides. CPE is primarily expressed in endocrine and neuroendocrine tissues including the brain pituitary and pancreas. Its expression in these areas suggests significant involvement in the processing and regulation of peptide hormones and neurotransmitters.
Biological function summary

CPE influences the maturation of peptide hormones and neurotransmitters converting inactive precursors into active forms. CPE does not function as part of a larger complex but acts independently to cleave the C-terminal residues. Its activity plays a role in the biosynthesis of hormones like insulin and proinsulin regulating energy homeostasis and metabolic processes.

Pathways

The activities of enzymes like CPE are integral to the prohormone and neuropeptide processing pathways. Within these pathways CPE works alongside other processing enzymes such as prohormone convertases to ensure proper hormone maturation and secretion. In these pathways CPE connects with proteins like chromogranin A which assists in the storage and trafficking of secretory granules in neuroendocrine cells.

Dysregulation of CPE has links to obesity and type 2 diabetes due to its role in peptide hormone processing. Altered CPE function can impair the maturation of insulin and other hormones contributing to these metabolic conditions. In addition relationships between CPE and other proteins such as proinsulin and insulin further highlight its significance in the pathology of these diseases.
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Product protocols

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Target data

Sorting receptor that directs prohormones to the regulated secretory pathway. Acts also as a prohormone processing enzyme in neuro/endocrine cells, removing dibasic residues from the C-terminal end of peptide hormone precursors after initial endoprotease cleavage.
See full target information Carboxypeptidase E
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websiteProtocolBooklet
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Complementary Products

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