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Human CPE ELISA Kit - Intracellular is a Sandwich (quantitative) ELISA kit for the measurement of Human CPE - Intracellular in Human in Tissue Extracts, Cell Lysate samples.

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Images

Sandwich ELISA - Human CPE ELISA Kit - Intracellular (AB309192), expandable thumbnail
  • Sandwich ELISA - Human CPE ELISA Kit - Intracellular (AB309192), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

125 - 8000 pg/mL

Assay time

1h 30m

Sensitivity

= 35 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Sorting receptor that directs prohormones to the regulated secretory pathway. Acts also as a prohormone processing enzyme in neuro/endocrine cells, removing dibasic residues from the C-terminal end of peptide hormone precursors after initial endoprotease cleavage.

Alternative names

What's included?

1 x 96 Tests
Components
10X Human CPE - Intracellular Capture Antibody
1 x 600 µL
10X Human CPE - Intracellular Detector Antibody
1 x 600 µL
10X Wash Buffer PT
1 x 20 mL
5X Cell Extraction Buffer PTR
1 x 10 mL
Antibody Diluent 4BI
1 x 6 mL
Human CPE - Intracellular Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seal
1 x 1 Unit
Pre-Coated 96-Well Microplate
1 x 1 Unit
Sample Diluent NS
1 x 12 mL
Stop Solution
1 x 12 mL
TMB Substrate
1 x 12 mL

Recommended products

Human CPE ELISA Kit - Intracellular is a Sandwich (quantitative) ELISA kit for the measurement of Human CPE - Intracellular in Human in Tissue Extracts, Cell Lysate samples.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 35 pg/mL

General recovery

104%

Precision

Intra assay

Sample

Extract

n

8

mean

-

SD

-

C.V.

2.6

Inter assay

Sample

Extract

n

3

mean

-

SD

-

C.V.

2

Recovery

Sample specific recovery

Sample type

Tissue Extracts

Average %

= 105

Range

99 - 108 %

Sample type

Cell Lysate

Average %

= 92

Range

89 - 95 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human CPE - Intracellular SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CPE - Intracellular protein in human cell lysates and tissue extracts. Quantitate Human CPE - Intracellular with 14 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Carboxypeptidase E (CPE) also known as carboxypeptidase H is an enzyme with a mass of about 53 kDa. It facilitates the removal of C-terminal amino acid residues from proteins and peptides. CPE is primarily expressed in endocrine and neuroendocrine tissues including the brain pituitary and pancreas. Its expression in these areas suggests significant involvement in the processing and regulation of peptide hormones and neurotransmitters.

Biological function summary

CPE influences the maturation of peptide hormones and neurotransmitters converting inactive precursors into active forms. CPE does not function as part of a larger complex but acts independently to cleave the C-terminal residues. Its activity plays a role in the biosynthesis of hormones like insulin and proinsulin regulating energy homeostasis and metabolic processes.

Pathways

The activities of enzymes like CPE are integral to the prohormone and neuropeptide processing pathways. Within these pathways CPE works alongside other processing enzymes such as prohormone convertases to ensure proper hormone maturation and secretion. In these pathways CPE connects with proteins like chromogranin A which assists in the storage and trafficking of secretory granules in neuroendocrine cells.

Associated diseases and disorders

Dysregulation of CPE has links to obesity and type 2 diabetes due to its role in peptide hormone processing. Altered CPE function can impair the maturation of insulin and other hormones contributing to these metabolic conditions. In addition relationships between CPE and other proteins such as proinsulin and insulin further highlight its significance in the pathology of these diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Human CPE ELISA Kit - Intracellular (ab309192), expandable thumbnail

    Sandwich ELISA - Human CPE ELISA Kit - Intracellular (ab309192)

    Interpolated concentration of native CPE was measured in duplicate at different sample concentrations. Undiluted samples are as follows: human brain cortex tissue extract 35 µg/mL and SH-SY5Y cell extract 75 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

  • Sandwich ELISA - Human CPE ELISA Kit - Intracellular (ab309192), expandable thumbnail

    Sandwich ELISA - Human CPE ELISA Kit - Intracellular (ab309192)

    Example of human CPE standard curve. Background-subtracted data values (mean +/- SD) are graphed.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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