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AB309192

Human CPE ELISA Kit - Intracellular

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Human CPE ELISA Kit - Intracellular is a Sandwich ELISA for the measurement of Human CPE - Intracellular in Human in Cell/Tissue Extracts samples.

View Alternative Names

Carboxypeptidase E, CPE, Carboxypeptidase H, Enkephalin convertase, Prohormone-processing carboxypeptidase, CPH

2 Images
Sandwich ELISA - Human CPE ELISA Kit - Intracellular (AB309192)
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Supplier Data

Sandwich ELISA - Human CPE ELISA Kit - Intracellular (AB309192)

Example of human CPE standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CPE ELISA Kit - Intracellular (AB309192)
  • sELISA

Supplier Data

Sandwich ELISA - Human CPE ELISA Kit - Intracellular (AB309192)

Interpolated concentration of native CPE was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human brain cortex tissue extract 35 µg/mL and SH-SY5Y cell extract 75 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 35 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB275882

Anti-CPE antibody [EPR23306-21]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPE antibody [EPR23306-21] (AB275882)

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Proteins & Peptides

AB139787

Recombinant Human CPE protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human CPE protein (His tag N-Terminus) (AB139787)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CPE - Intracellular SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CPE - Intracellular protein in human cell lysates and tissue extracts. Quantitate Human CPE - Intracellular with 14 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "99 - 108 %", "average": "= 105" }, { "sample": "Cell Lysate", "range": "89 - 95 %", "average": "= 92" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309192-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB309192-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB309192-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB309192-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB309192-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS", "number":"AB309192-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB309192-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CPE - Intracellular Capture Antibody", "number":"AB309192-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CPE - Intracellular Lyophilized Recombinant Protein", "number":"AB309192-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR", "number":"AB309192-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CPE - Intracellular Detector Antibody", "number":"AB309192-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB309192-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Carboxypeptidase E (CPE) also known as carboxypeptidase H is an enzyme with a mass of about 53 kDa. It facilitates the removal of C-terminal amino acid residues from proteins and peptides. CPE is primarily expressed in endocrine and neuroendocrine tissues including the brain pituitary and pancreas. Its expression in these areas suggests significant involvement in the processing and regulation of peptide hormones and neurotransmitters.
Biological function summary

CPE influences the maturation of peptide hormones and neurotransmitters converting inactive precursors into active forms. CPE does not function as part of a larger complex but acts independently to cleave the C-terminal residues. Its activity plays a role in the biosynthesis of hormones like insulin and proinsulin regulating energy homeostasis and metabolic processes.

Pathways

The activities of enzymes like CPE are integral to the prohormone and neuropeptide processing pathways. Within these pathways CPE works alongside other processing enzymes such as prohormone convertases to ensure proper hormone maturation and secretion. In these pathways CPE connects with proteins like chromogranin A which assists in the storage and trafficking of secretory granules in neuroendocrine cells.

Dysregulation of CPE has links to obesity and type 2 diabetes due to its role in peptide hormone processing. Altered CPE function can impair the maturation of insulin and other hormones contributing to these metabolic conditions. In addition relationships between CPE and other proteins such as proinsulin and insulin further highlight its significance in the pathology of these diseases.
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Product protocols

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Target data

Sorting receptor that directs prohormones to the regulated secretory pathway. Also acts as a prohormone processing enzyme in neuro/endocrine cells, removing dibasic residues from the C-terminal end of peptide hormone precursors after initial endoprotease cleavage.
See full target information Carboxypeptidase E
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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