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AB283984

Human CPLX1 ELISA Kit

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Human CPLX1 ELISA Kit is a Sandwich ELISA for the measurement of Human CPLX1 in Human in Cell/Tissue Extracts samples.

View Alternative Names

Complexin-1, Complexin I, Synaphin-2, CPX I, CPLX1

1 Images
Sandwich ELISA - Human CPLX1 ELISA Kit (AB283984)
  • sELISA

Supplier Data

Sandwich ELISA - Human CPLX1 ELISA Kit (AB283984)

Example of Human CPLX1 standard curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.8 ng/mL

Range

4.688 - 300 ng/mL

Assay time

5h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB231347

Anti-CPLX1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPLX1 antibody (AB231347)

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Proteins & Peptides

AB79148

Recombinant Human CPLX1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human CPLX1 protein (His tag N-Terminus) (AB79148)

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Primary Antibodies

AB59208

Anti-S6K1 (phospho T229) antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 (phospho T229) antibody (AB59208)

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Primary Antibodies

AB305299

Anti-TRPV1 antibody [EPR25057-67]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody [EPR25057-67] (AB305299)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human CPLX1 ELISA Kit (ab283984) is designed for detection of complexin-1 (CPLX1) in human cell lysate and tissue samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human complexin-1 in approximately 5 hours.

A polyclonal antibody specific for human complexin-1 has been pre-coated onto a 96-well microplate with removable strips. CPLX1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human complexin-1, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283984-1x96Tests", "assetComponentDetails": [ { "size":"1 x 150 µL", "name":"40X Biotinylated Human CPLX1 Antibody", "number":"AB283984-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"CPLX1 Standard", "number":"AB283984-CMP03", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB283984-CMP06", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB283984-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB283984-CMP04", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB283984-CMP08", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB283984-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Diluent M Concentrate", "number":"AB283984-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human CPLX1 Microplate", "number":"AB283984-CMP05", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB283984-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target CPLX1 also known as Complexin-1 functions in the mechanical control of presynaptic vesicle release. It weighs approximately 16 kDa and finds expression primarily in the central nervous system. CPLX1 interacts with the SNARE complex playing a role in neurotransmitter release. This protein ensures precise regulation at synaptic junctions which are important for efficient neuronal communication.
Biological function summary

Complexin-1 acts as an essential regulator of synaptic exocytosis. It stabilizes the SNARE complex inhibiting premature synaptic vesicle fusion while also preparing the vesicles for rapid release upon calcium influx. In synaptic terminals Complexin-1 ensures quick and precise neurotransmitter release therefore supporting synaptic plasticity and signal transduction essential for learning and memory. Complexin-1 forms complexes with proteins like synaptobrevin and syntaxin participating in the excitatory and inhibitory synaptic transmission.

Pathways

Complexin-1 plays a significant role in the synaptic vesicle cycle and neurotransmitter secretion pathways. It interacts with proteins in the SNARE complex notably SNAP-25 and synaptotagmin facilitating neurotransmitter release upon stimulus. Its function in these pathways supports the maintenance of synaptic efficacy and neuronal circuitry critical for normal nervous system operation and responses to environmental stimuli.

Abnormalities in Complexin-1 expression or function link to neurological conditions like epilepsy and schizophrenia. Both of these disorders involve disrupted synaptic transmission and altered neurotransmitter release. Additionally Complexin-1 has connections with the protein alpha-synuclein implicated in neurodegenerative diseases such as Parkinson's disease. These associations highlight the importance of Complexin-1 in maintaining synaptic function and its potential as a therapeutic target.
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Product protocols

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Target data

Positively regulates a late step in exocytosis of various cytoplasmic vesicles, such as synaptic vesicles and other secretory vesicles (PubMed : 21785414). Organizes the SNAREs into a cross-linked zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion, thereby preventing SNAREs from releasing neurotransmitters until an action potential arrives at the synapse (PubMed : 21785414). Also involved in glucose-induced secretion of insulin by pancreatic beta-cells. Essential for motor behavior.
See full target information CPLX1
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websiteProtocolBooklet
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Product promise

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