Human CPLX1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CPLX1 in Human in Cell culture supernatant samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.688 - 300 ng/mL
- Sensitivity
- = 4 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Positively regulates a late step in exocytosis of various cytoplasmic vesicles, such as synaptic vesicles and other secretory vesicles (PubMed:21785414). Organizes the SNAREs into a cross-linked zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion, thereby preventing SNAREs from releasing neurotransmitters until an action potential arrives at the synapse (PubMed:21785414). Also involved in glucose-induced secretion of insulin by pancreatic beta-cells. Essential for motor behavior.
Alternative names
Complexin-1, Complexin I, Synaphin-2, CPX I, CPLX1
What's included?
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Human CPLX1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CPLX1 in Human in Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.688 - 300 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 4 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 60 | mean - | SD - | C.V. 3.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 60 | mean - | SD - | C.V. 9.2 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
The Human CPLX1 ELISA Kit (ab283984) is designed for detection of complexin-1 (CPLX1) in human cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human complexin-1 in approximately 5 hours.
A polyclonal antibody specific for human complexin-1 has been pre-coated onto a 96-well microplate with removable strips. CPLX1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human complexin-1, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target CPLX1 also known as Complexin-1 functions in the mechanical control of presynaptic vesicle release. It weighs approximately 16 kDa and finds expression primarily in the central nervous system. CPLX1 interacts with the SNARE complex playing a role in neurotransmitter release. This protein ensures precise regulation at synaptic junctions which are important for efficient neuronal communication.
- Biological function summary
Complexin-1 acts as an essential regulator of synaptic exocytosis. It stabilizes the SNARE complex inhibiting premature synaptic vesicle fusion while also preparing the vesicles for rapid release upon calcium influx. In synaptic terminals Complexin-1 ensures quick and precise neurotransmitter release therefore supporting synaptic plasticity and signal transduction essential for learning and memory. Complexin-1 forms complexes with proteins like synaptobrevin and syntaxin participating in the excitatory and inhibitory synaptic transmission.
- Pathways
Complexin-1 plays a significant role in the synaptic vesicle cycle and neurotransmitter secretion pathways. It interacts with proteins in the SNARE complex notably SNAP-25 and synaptotagmin facilitating neurotransmitter release upon stimulus. Its function in these pathways supports the maintenance of synaptic efficacy and neuronal circuitry critical for normal nervous system operation and responses to environmental stimuli.
- Associated diseases and disorders
Abnormalities in Complexin-1 expression or function link to neurological conditions like epilepsy and schizophrenia. Both of these disorders involve disrupted synaptic transmission and altered neurotransmitter release. Additionally Complexin-1 has connections with the protein alpha-synuclein implicated in neurodegenerative diseases such as Parkinson's disease. These associations highlight the importance of Complexin-1 in maintaining synaptic function and its potential as a therapeutic target.
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1 product image
Sandwich ELISA - Human CPLX1 ELISA Kit (ab283984)
Example of Human CPLX1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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