Human CREG1/CREG ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CREG1/CREG in Human in Cell culture supernatant, Plasma, Serum samples.
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Key facts
- Detection method
Colorimetric
- Sample types
Cell culture supernatant, Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
0.45 - 100 ng/mL
- Sensitivity
= 0.45 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May contribute to the transcriptional control of cell growth and differentiation. Antagonizes transcriptional activation and cellular transformation by the adenovirus E1A protein. The transcriptional control activity of cell growth requires interaction with IGF2R.
Alternative names
Protein CREG1, Cellular repressor of E1A-stimulated genes 1, CREG1, UNQ727/PRO1409, CREG
What's included?
Recommended products
Human CREG1/CREG ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CREG1/CREG in Human in Cell culture supernatant, Plasma, Serum samples.
Alternative names
Protein CREG1, Cellular repressor of E1A-stimulated genes 1, CREG1, UNQ727/PRO1409, CREG
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture supernatant, Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
0.45 - 100 ng/mL
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 0.45 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 85.44 | Range 72 - 112 % |
Sample type Plasma | Average % = 75.29 | Range 68 - 93 % |
Sample type Cell culture media | Average % = 96.09 | Range 78 - 109 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Human CREG1/CREG ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CREG1/CREG in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CREG1/CREG coated on a 96-well plate.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human AMIGO, Aminopeptidase LRAP (ERAP2), Amnionless, Arylsulfatase A, Bcl-w, CD109, CD157, CD34, CD83, CLEC1, CLEC10A (CD301), CMG-2, Cystatin SN, Cytokeratin-8, Dectin-1 (CLEC7A), Desmocollin-3, Endoglycan, Galectin-4, HAPLN1, Jagged 1, Langerin (CD207), Lumican, Matriptase (ST14), MEP1B, Nectin-3, OX40 (TNFRSF4), OX40 Ligand (TNFSF4), p27, Pappalysin2, Plexin B3, Plexin D1, proGRP, PSA-total, Reg1B, RGM-A, ROBO2, Spinesin, TWEAK R (TNFRSF12), ULBP-3.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CREG1 also known as CREG is a glycoprotein that interacts with transcription machinery to regulate cellular growth and morphology. The protein has a molecular mass around 25 kDa. It is expressed in various tissues with the highest expression noted in reproductive organs and the heart. CREG1 acts as a transcriptional regulator by binding to DNA elements and specific proteins such as the retinoblastoma protein (pRb). This binding contributes to its role in modulating gene expression related to cell division and differentiation.
- Biological function summary
CREG1 influences cell proliferation and differentiation processes. It forms part of a complex network involving transcription factors and other proteins that govern these cellular functions. Through its interaction with pRb CREG1 can repress certain gene activities while activating others allowing it to maintain a balance between cellular growth and maturation. This balance is essential for normal development and tissue maintenance.
- Pathways
CREG1 has a significant role in TGF-beta and E2F-dependent signaling. These pathways are key in cell cycle regulation apoptosis and stem cell biology. Through the E2F pathway CREG1 influences the transcriptional regulation and aids in controlling cell division. CREG1's interaction with pRb and E2F exemplifies how it modulates the progression of the cell cycle playing a part in pathways that determine cell identity and quiescence.
- Associated diseases and disorders
Irregular CREG1 activity links to cancer and cardiac disorders. Specifically abnormal expression levels can lead to cancerous growth through dysregulated cell cycle control partly mediated by its relationship with pRb. In cardiac disorders alterations in CREG1 expression are associated with heart disease as it is involved in cardiac cell homeostasis. These connections highlight the importance of CREG1 in maintaining normal cellular functions in vital organs.
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1 product image
Sandwich ELISA - Human CREG1/CREG ELISA Kit (ab313539)
Example of standard curve using ab313539. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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Product protocols
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