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AB313540

Human CRELD2 ELISA Kit

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Human CRELD2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CRELD2 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

UNQ185/PRO211, CRELD2, Protein disulfide isomerase CRELD2, Cysteine-rich with EGF-like domain protein 2

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Sandwich ELISA - Human CRELD2 ELISA Kit (AB313540)
  • sELISA

Supplier Data

Sandwich ELISA - Human CRELD2 ELISA Kit (AB313540)

Example of standard curve using ab313540. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.13 ng/mL

Range

0.137 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CRELD2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CRELD2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CRELD2 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Adenosine Deaminase/ADA, AIF, AKR1C4 , ASAH2, BCL-2, BID, Calreticulin, Calreticulin-2, CD314 (NKG2D), CD39L3/ENTPD3, CD51, CD99-L2, CDC25B, Cerberus 1, CHST2, Cochlin, DC SIGNR/CD299, eNOS, ENPP-2/Autotaxin, FABP4, Fc epsilon RI, FGF R5, GALNT2, GALNT3, GIF, GPR111, beta-Glucuronidase/GUSB, Inhibin A, LILRB4/CD85k/ILT3, Neuroglycan C/CSPG5, NKp46/NCR1, Neuronal Pentraxin R/NPTXR, ROR2, SCCA2, Siglec-2/CD22, SIRP alpha/CD172a, SorCS1,Trypsin 1/PRSS1, Trypsin 3/PRSS3.

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Recovery

[ { "sample": "Serum", "range": "132 - 145 %", "average": "= 138.8" }, { "sample": "Plasma", "range": "128 - 149 %", "average": "= 140" }, { "sample": "Cell culture media", "range": "134 - 145 %", "average": "= 140.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313540-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin", "number":"AB313540-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human CRELD2 Protein Standard", "number":"AB313540-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313540-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human CRELD2 Antibody-coated ELISA Plate", "number":"AB313540-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human CRELD2 Detection Antibody", "number":"AB313540-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313540-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313540-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313540-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ELISA Microplate Bag", "number":"AB313540-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cysteine-Rich with EGF-Like Domains 2 (CRELD2) is a protein encoded by the CRELD2 gene also known as Cysteine-Rich with EGF-Like Domains Protein 2. The protein consists of 362 amino acids and has a molecular mass of approximately 39 kDa. CRELD2 expresses mainly in the endoplasmic reticulum and can also be found in many tissues with notable levels in neural tissues and cardiac muscle. This protein functions as a chaperone influencing the folding and stability of other proteins within the cell.
Biological function summary

CRELD2 plays a significant role in the endoplasmic reticulum stress response a process important for maintaining cellular homeostasis. The protein modulates cellular stress pathways and associates with several other chaperone proteins assisting in the proper folding of newly synthesized proteins. It does not associate with a particular protein complex but functions independently to ensure efficient protein synthesis and folding under stress conditions.

Pathways

CRELD2 integrates primarily with the unfolded protein response (UPR) pathway activated during ER stress to restore normal function by halting protein translation and facilitating protein folding. In this pathway CRELD2 interacts with key proteins such as ATF6 and IRE1. These interactions enhance its role in correcting misfolded proteins and prevent the accumulation of toxic protein aggregates within cells.

CRELD2 links to several conditions especially those related to disrupted protein homeostasis like Alzheimer's disease and cardiac abnormalities. Disruptions in CRELD2 expression can impact protein folding and stress responses which contributes to the pathogenesis of these diseases. It also connects with proteins such as amyloid precursor protein (APP) and cardiac myosin emphasizing its role in neuronal and cardiac health.
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Product protocols

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Target data

Protein disulfide isomerase (By similarity). Might play a role in the unfolded protein response (By similarity). May regulate transport of alpha4-beta2 neuronal acetylcholine receptor (PubMed : 16238698).
See full target information CRELD2
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