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AB313676

Human CRHBP ELISA Kit

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Human CRHBP ELISA Kit is a Sandwich ELISA for the measurement of Human CRHBP in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

CRFBP, CRHBP, Corticotropin-releasing factor-binding protein, CRF-BP, CRF-binding protein, Corticotropin-releasing hormone-binding protein, CRH-BP

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Sandwich ELISA - Human CRHBP ELISA Kit (AB313676)
  • sELISA

Supplier Data

Sandwich ELISA - Human CRHBP ELISA Kit (AB313676)

Example of standard curve using ab313676. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.07 ng/mL

Range

0.07 - 20 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Proteins & Peptides

AB152064

Recombinant Human CRHBP protein

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CRHBP ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CRHBP in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CRHBP coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "84 - 134 %", "average": "= 107.2" }, { "sample": "Plasma", "range": "82 - 126 %", "average": "= 114.3" }, { "sample": "Cell culture media", "range": "77 - 140 %", "average": "= 109.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313676-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Biotinylated Human CRHBP Detection Antibody", "number":"AB313676-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313676-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB313676-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human CRHBP Protein Standard", "number":"AB313676-CMP10", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313676-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313676-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313676-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human CRHBP Antibody-coated ELISA Plate", "number":"AB313676-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CRHBP also known as Corticotropin-Releasing Hormone Binding Protein plays a role in regulating the activity of corticotropin-releasing hormone (CRH) by binding to it with high affinity. The binding protein has a molecular mass of approximately 37 kDa. It is mainly found in the brain and pituitary gland. CRHBP controls the actions of CRH by sequestering it preventing CRH from interacting with its receptors. This modulation is essential for maintaining the balance of CRH's activity.
Biological function summary

CRHBP modulates the stress response by binding to CRH and limiting its availability to CRHR1 and CRHR2 receptors. By doing so it reduces the downstream signaling that would be induced by free CRH which affects the release of adrenocorticotropic hormone (ACTH) from the pituitary. This regulation is important for controlling how the body responds to stress. CRHBP does not form a larger protein complex but operates by interacting directly with CRH in the extracellular space.

Pathways

CRHBP functions within the hypothalamic-pituitary-adrenal (HPA) axis which plays an important role in the body's response to stress. By regulating CRH availability CRHBP indirectly influences the cortisol production pathway. This is important because cortisol is an important hormone in modulating metabolism and immune responses. Furthermore CRHBP interactions connect it to CRH which also communicates with other stress-related proteins such as CRHR1 and CRHR2 influencing their activity within the HPA axis.

CRHBP has been linked to conditions such as depression and anxiety disorders. The protein's role in regulating stress responses makes it relevant for understanding these conditions. Changes in CRHBP expression or function may alter CRH availability impacting mood regulation and psychological resilience. Additionally CRH the ligand for CRHBP is implicated in these disorders by influencing pathways that affect emotional behavior and stress tolerance.
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Product protocols

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Target data

Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy.
See full target information CRHBP
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websiteProtocolBooklet
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Product promise

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