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AB313677

Human CRIM1 ELISA Kit

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Human CRIM1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CRIM1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

S52, UNQ1886/PRO4330, CRIM1, Cysteine-rich motor neuron 1 protein, CRIM-1, Cysteine-rich repeat-containing protein S52

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Sandwich ELISA - Human CRIM1 ELISA Kit (AB313677)
  • sELISA

Supplier Data

Sandwich ELISA - Human CRIM1 ELISA Kit (AB313677)

Example of standard curve using ab313677. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.041 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CRIM1 (Cysteine-rich motor neuron 1) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CRIM1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CRIM1 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human 4-1BB Ligand (TNFSF9), Activin RIIB, Aminopeptidase P2 (XPNPEP2), BAMBI (NMA), BOC, Brevican, Carbonic Anhydrase XII, Carboxypeptidase A2, CD300c (LMIR2), CD320 (TCblR, 8D6A), CDNF, CDO, CHST1, CHST4, CILP-1, CNTF R alpha, CRTAC1, CXADR, Dopa Decarboxylase (DDC), DPPII (QPP, DPP7), DSPG3, EMR2, FCAR (CD89), FCRL1 (FcRH1), FCRL2 (FcRH2), Gas6, GPR56, GPVI, Hepsin, ILT2 (CD85j), Jagged 2, Kirrel3 (NEPH2), KLF4, LAIR1, LAMP, LAMP1 (CD107a), MDGA1, MIS RII, Neurexin 3.

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Recovery

[ { "sample": "Serum", "range": "123 - 145 %", "average": "= 133.9" }, { "sample": "Plasma", "range": "101 - 127 %", "average": "= 116.1" }, { "sample": "Cell culture media", "range": "100 - 115 %", "average": "= 109.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313677-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin", "number":"AB313677-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human CRIM1 Protein Standard", "number":"AB313677-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human CRIM1 Detection Antibody", "number":"AB313677-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human CRIM1 Antibody-coated ELISA Plate", "number":"AB313677-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313677-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313677-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313677-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313677-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CRIM1 also known as cysteine-rich motor neuron 1 protein has a molecular mass of around 110 kDa. As a transmembrane protein it localizes mainly to the endoplasmic reticulum. Researchers have detected its expression in various tissues including the retina kidneys brain and spinal cord. It contains a distinctive cysteine-rich motif and plays roles in cellular adhesion and growth factor regulation.
Biological function summary

The function of CRIM1 extends to the modulation of growth factor activity particularly by interacting with bone morphogenetic proteins (BMPs). This interaction suggests its potential involvement in developmental processes. Studies have indicated that CRIM1 might participate in forming protein complexes related to cell surface receptors influencing cell signaling pathways that govern growth and differentiation.

Pathways

CRIM1's involvement in BMP signaling and angiogenesis highlights its significance within these biological pathways. BMPs which are growth factors play a part in bone and cartilage development therefore CRIM1's regulatory action can affect these processes. Additionally CRIM1 interacts with proteins like noggin in the BMP pathway to modulate cellular responses to extracellular signals contributing to developmental and morphogenic outcomes.

CRIM1 links to congenital abnormalities and ocular disorders. Its known interaction with BMPs associates it with conditions like kidney dysplasia where the disruption of normal signaling can lead to organ malformations. In the context of eye development CRIM1's interaction with BMPs and noggin is important for retinal and lens development. Understanding CRIM1's role in these pathways could offer insights into therapeutic targets for congenital and degenerative diseases.
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Product protocols

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Target data

May play a role in CNS development by interacting with growth factors implicated in motor neuron differentiation and survival. May play a role in capillary formation and maintenance during angiogenesis. Modulates BMP activity by affecting its processing and delivery to the cell surface.
See full target information CRIM1
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websiteProtocolBooklet
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