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AB245719

Human Cripto ELISA Kit

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Human Cripto ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Cripto with a sensitivity of 9.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CRIPTO-1, TDGF1, CRIPTO, Protein Cripto, Cripto-1 growth factor, Epidermal growth factor-like cripto protein CR1, Teratocarcinoma-derived growth factor 1, CRGF

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Sandwich ELISA - Human Cripto ELISA Kit (AB245719)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cripto ELISA Kit (AB245719)

Interpolated concentrations of spiked Cripto in human serum and plasma samples. The concentrations of Cripto were measured in duplicates and interpolated from the Cripto standard curves. Undiluted samples are as follows : serum 12.5%, plasma (citrate) 12.5%, and plasma (EDTA) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Cripto ELISA Kit (AB245719)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cripto ELISA Kit (AB245719)

Example of human Cripto standard curve in Sample Diluent NS. The Cripto standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Cripto ELISA Kit (AB245719)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cripto ELISA Kit (AB245719)

Interpolated concentrations of native Cripto in human NCCIT supernatant samples. The concentrations of Cripto were measured in duplicates, interpolated from the Cripto standard curves and corrected for sample dilution. Undiluted samples are as follows : NCCIT supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cripto concentration was determined to be 1.58 ng/mL in NCCIT supernatant.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 9.6 pg/mL

Range

26.56 - 1700 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB108391

Anti-Cripto1/CRIPTO antibody [EPNCIR106A]

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)

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Proteins & Peptides

AB314699

Recombinant Human TDGF1 Protein

Mass Spectrometry - Recombinant Human TDGF1 Protein (AB314699)

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Primary Antibodies

AB207413

Anti-GSTA1 antibody [EPR19969]

RabMAb|Recombinant

Immunoprecipitation - Anti-GSTA1 antibody [EPR19969] (AB207413)

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Multiplex Kits

AB283852

PI3K/AKT signalling pathway panel

Recombinant

Immunocytochemistry - PI3K/AKT signalling pathway panel (AB283852)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Cripto ELISA Kit (ab245719) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cripto protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cripto with 9.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Protein", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": null, "average": "= 95" }, { "sample": "Serum", "range": null, "average": "= 86" }, { "sample": "EDTA Plasma", "range": null, "average": "= 86" }, { "sample": "Citrate plasma", "range": null, "average": "= 86" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab245719-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB245719-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB245719-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB245719-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB245719-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB245719-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB245719-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Cripto Lyophilized Recombinant Protein", "number":"AB245719-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cripto Capture Antibody", "number":"AB245719-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB245719-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cripto Detector Antibody", "number":"AB245719-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by TMEM8A modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells (PubMed : 27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed : 11909953).
See full target information CRIPTO
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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