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AB213768

Human CRISP3 ELISA Kit

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(1 Publication)

Human CRISP3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CRISP3 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Cysteine-rich secretory protein 3, CRISP-3, Specific granule protein of 28 kDa, SGP28, CRISP3

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Sandwich ELISA - Human CRISP3 ELISA Kit (AB213768)
  • sELISA

Supplier Data

Sandwich ELISA - Human CRISP3 ELISA Kit (AB213768)

Human CRISP3 ELISA Kit (ab213768) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

The Human CRISP3 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213768) is designed for the quantitative measurement of Human CRISP3 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine.

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CRISP3 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: N21-Y245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRISP3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CRISP3 amount of sample captured in plate.

Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "408 pg/mL", "standardDeviation": "26.11", "coefficientOfVariability": "= 6.4" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "1764 pg/mL", "standardDeviation": "95.26", "coefficientOfVariability": "= 5.4" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "110 pg/mL", "standardDeviation": "4.51", "coefficientOfVariability": "= 4.1" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "119 pg/mL", "standardDeviation": "5.59", "coefficientOfVariability": "= 4.7" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "383 pg/mL", "standardDeviation": "23.75", "coefficientOfVariability": "= 6.2" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "1477 pg/mL", "standardDeviation": "66.47", "coefficientOfVariability": "= 4.5" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CRISP3 also known as Cysteine-Rich Secretory Protein 3 is a protein key in various physiological processes. It typically weighs about 28 kDa. Found in a range of tissues but most prominently in the salivary glands pancreas and prostate. Researchers also detect CRISP3 in neutrophils. Its secretion to the extracellular environment after synthesis helps facilitate its functional roles. Scientists use ELISA assays to quantify its expression.
Biological function summary

CRISP3 participates in host defense mechanisms and inflammation. It is a part of antimicrobial complexes that safeguard the body against microbial invasion. This protein can bind to other molecules and disrupt microbial membranes. It is believed to be involved in the innate immune response. CRISP3 also appears in granules of neutrophils where it functions during pathogen encounters.

Pathways

CRISP3 interacts in immune response pathways that help buffer the body against pathogenic challenges. It aligns with the immune system's defense activities and might impact the signaling and regulatory pathways of inflammation. CRISP3's interactions with other proteins like defensins enhance its role in these immune pathways.

CRISP3 shows potential involvement in prostate cancer and Sjögren's syndrome. Its overexpression associates with prostate cancer progression and alterations in its levels might affect cancer-related proteins like PSA (prostate-specific antigen). In the context of Sjögren's syndrome CRISP3's expression changes may link to saliva secretion dysregulation impacting mucosal defense and inflammatory proteins involved in the disorder.
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Product protocols

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Target data

See full target information Cysteine-rich secretory protein 3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioscience reports 39: PubMed31300526

2019

Investigation of serum proteome homeostasis during radiation therapy by a quantitative proteomics approach.

Applications

Unspecified application

Species

Unspecified reactive species

Amira Ouerhani,Giovanni Chiappetta,Oussema Souiai,Halima Mahjoubi,Joelle Vinh
View all publications
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